[1]胡庆峰,赵春芳,张善磊,等.2种不同Wx等位基因型水稻粒质量和蒸煮食味品质的差异[J].江苏农业学报,2017,(05):961-967.[doi:doi:10.3969/j.issn.1000-4440.2017.05.001]
　HU Qing-feng,ZHAO Chun-fang,ZHANG Shan-lei,et al.Differences of grain weight and eating and cooking quality between two different Wx alleles in rice[J].,2017,(05):961-967.[doi:doi:10.3969/j.issn.1000-4440.2017.05.001]
点击复制

2种不同Wx等位基因型水稻粒质量和蒸煮食味品质的差异()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年05期
页码:
961-967
栏目:
遗传育种·生理生化
出版日期:
2017-10-30

文章信息/Info

Title:
Differences of grain weight and eating and cooking quality between two different Wx alleles in rice
作者:
胡庆峰1; 2; 赵春芳2; 张善磊1; 2; 周丽慧2; 赵凌2; 梁文化2; 赵庆勇2; 陈涛2; 张亚东2; 朱镇2; 姚姝2; 王才林1; 2
（1.南京农业大学农学院/江苏省现代作物生产协同创新中心，江苏南京210095；2.江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心，江苏南京210014）
Author(s):
HU Qing-feng1; 2; ZHAO Chun-fang2; ZHANG Shan-lei1; 2; ZHOU Li-hui2; ZHAO Ling2; LIANG Wen-hua2; ZHAO Qing-yong2; CHEN Tao2; ZHANG Ya-dong2; ZHU Zhen2; YAO Shu2; WANG Cai-lin1; 2
(1.College of Agriculture, Nanjing Agricultural University/Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210095, China；2.Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R&D Center/Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, China)
关键词:
水稻; Wx等位基因; 千粒质量; 直链淀粉含量; 蒸煮食味品质
Keywords:
rice; Wx allele; thousand-grain weight; amylose content; cooking and eating quality
分类号:
S511
DOI:
doi:10.3969/j.issn.1000-4440.2017.05.001
文献标志码:
A
摘要:
以携带Wxmq基因的宁0145为母本,携带Wxb基因的武运粳21为父本构建的F3和F4群体为材料，利用分子标记对群体进行基因型分类，测定籽粒千粒质量、直链淀粉含量、胶稠度及RVA谱特征值等性状，分析不同Wx等位基因型各性状值的差异。结果表明：WxmqWxmq与WxbWxb基因型间千粒质量和稻米蒸煮食味品质性状的差异均达到显著水平，其中WxbWxb基因型的稻米千粒质量、直链淀粉含量、胶稠度、热浆粘度和冷胶粘度均大于WxmqWxmq基因型，而崩解值小于WxmqWxmq基因型；相关性分析结果表明，籽粒质量与直链淀粉含量呈极显著正相关。对不同基因型的淀粉粒精细结构和淀粉密度的测定结果表明，淀粉密度差异可能是导致千粒质量不同的主要原因。携带WxmqWxmq基因型的稻米因具有较低直链淀粉含量、胶稠度、热浆粘度、冷胶粘度、消减值、起浆温度和峰值时间，较高崩解值，而使米饭呈现柔软性和弹性等较好食味品质的特点。
Abstract:
Rice Ning 0145 carrying Wxmq allele and Wuyunjing 21 carrying Wxb allele were used as parents to construct the F3 and F4 generations. Through detecting the genotype, measuring the thousand-grain weight(TGW), the value of amylose content (AC), gel consistency (GC) and RVA profile of the F3 and F4 generations, the effects of different Wx alleles on grain weight and eating and cooking quality(ECQ) were studied. The results show that there were significant differences in TGW and ECQ between different Wx alleles. The thousand-grain weight and the value of amylose content, gel consistency, hot paste viscosity (HPV) and cool paste viscosity (CPV) of the rice with WxbWxb genotype were greater than those with WxmqWxmq, but the value of breakdown value (BDV) was smaller. A significant positive correlation was shown between grain weight and amylose content value. The differences in starch density might be responsible for the differences in TGW. Rice with WxmqWxmq genotype had a lower value of amylose content, gel consistency, hot paste viscosity, cool paste viscosity, setback value, peak time and pasting temperature and a higher breakdown value, which have contributed to the good taste quality represented by softness and elasticity.

参考文献/References:

[1]陈楚.水稻直链淀粉的遗传及其应用研究[D].合肥：安徽农业大学，2003.
[2]陈静．江苏省水稻食味改良育种研究进展[J]．江苏农业科学，2015，43(12)：77-80.
[3]张益彬，杜永林，苏祖芳，等.无公害优质稻米生产[M].上海：上海科学技术出版社，2003.
[4]乔中英，陈培峰，黄萌，等．密度、氮肥水平对粳稻苏香粳3号淀粉黏滞性的影响[J]. 江苏农业科学，2015,43(7):69-70.
[5]SATO H，SUZUKI Y，SAKAI M，et al．Molecular characterization of Wx-mq，a novel mutant gene for low amylose content in endosperm of rice (Oryza sativa L.) [J]. Breeding Sci，2002，52(2)：131-135．
[6]LIU L，MA X，LIU S，et al. Identification and characterization of a novel Waxy allele from a Yunnan rice landrace[J]. Plant Mol Biol，2009，71(6)：609-626.
[7]朱霁晖，张昌泉，顾铭洪，等.水稻Wx基因的等位变异及育种利用研究进展[J]. 中国水稻科学，2015，29(4)：431-438.
[8]于新，张亚东，朱镇，等.携带Wx-mq基因水稻新品种(系)稻米RVA谱特征及与直链淀粉含量的相关性[J].华北农学报，2013， 28 (5)：53-58.
[9]TAKEDA K. Influence of waxy character on grain size[J].Japan J Breed，1975，25 (2)：87-92.
[10]TAKEDA K. Difference between the size of waxy and non-waxy dernal in the F1 rice plant[J]. Japan J Breed，1978 ，28 (3)：225 -233.
[11]MURRAY M G，THOMPSON W F.Rapid isolation of high-molecular weight plant DNA[J].Nucleic Acids Research，1980，8(19)：4321-4325．
[12]陈涛，骆名瑞，张亚东，等.利用四引物扩增受阻突变体系PCR技术检测水稻低直链淀粉含量基因Wx-mq[J].中国水稻科学，2013，27(5)：529-534.
[13]CHEN X， TEMNYKH S， XU Y， et al. Development of a microsatellite framework map providing genome-wide coverage in rice(Oryza sativa L.)[J]. Theor Appl Genet，1997， 95(4):553-567.
[14]中华人民共和国农业部.标准米质测定方法NY147-88[S].北京：中国标准出版社，1988：4-6.
[15]TIAN Z X，QIAN Q，LIU Q Q，et al. Allelic diversity in rice starch biosynthesis pathway leads to a diverse array of rice eating and cooking qualities[J]. Proc Natl Acad Sci，2009，106：21760-21765.
[16]莫惠栋.我国稻米品质的改良[J].中国农业科学，1993，26(4)：8-14.
[17]BAO J S，HE P，LI S G，et al. Comparative mapping quantitative trait loci controlling the cooking and eating quality of rice[J]. Sci Agric Sin，2000，33(8)：8-13.
[18]隋炯明，李欣，严松，等.稻米淀粉RVA特征谱与品质性状相关性研究[J].中国农业科学，2005，38(4)：657-663.
[19]王才林，陈涛，张亚东，等.通过分子标记辅助选择培育优良食味水稻新品种[J].中国水稻科学，2009，23(1)：25-30.
[20]王才林，张亚东，朱镇，等.优良食味粳稻新品种南粳5055的选育及利用[J].农业科技通讯，2012，45(2)：84-87.
[21]王才林，张亚东，朱镇，等.优良食味粳稻新品种南粳9108的选育及利用[J].江苏农业科学，2013，41(9)：86-88.
[22]徐云碧.水稻蜡质基因对产量构成性状的影响[J].作物学报，1989，15(3)：237-241.
[23]刘巧泉，王兴稳，陈秀花，等.转反义Wx基因糯稻的显性遗传及对稻米粒重的效应分析[J].中国农业科学，2002，35(2)：117-122.
[24]严菊强，徐均焕，薛庆中.水稻糯质基因对胚乳淀粉性状的影响[J].作物学报，1996， 22 (5)：638-640.
[25]ASHIKARI M，SAKAKIBARA H，LIN S，et al. Cytokinin oxidase regulates rice grain production[J]. Science，2005，309(5735)：741-745.
[26]XUE W，XING Y，WENG X，et al. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice[J]. Nature Genetics，2008，40(6)：761.
[27]LI X，QIAN Q，FU Z，et al. Control of tillering in rice.[J]. Nature，2003，422(6932)：618.
[28]FAN C，XING Y，MAO H，et al. GS3，a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein[J]. Theoretical and Applied Genetics，2006，112(6)：1164-1171.

相似文献/References:

[1]王士磊,丁正权,黄海祥.水稻隐性早熟突变体ref早熟性的遗传分析和基因定位[J].江苏农业学报,2016,(04):721.[doi:10.3969/j.issn.100-4440.2016.04.001]
　WANG Shi-lei,DING Zheng-quan,HUANG Hai-xiang.Inheritance and gene mapping of recessive earliness in rice (Oryza sativa L.)[J].,2016,(05):721.[doi:10.3969/j.issn.100-4440.2016.04.001]
[2]王在满,郑乐,张明华,等.不同播种方式对直播水稻倒伏指数和根系生长的影响[J].江苏农业学报,2016,(04):725.[doi:10.3969/j.issn.100-4440.2016.04.002]
　WANG Zai-man,ZHENG Le,ZHANG Ming-hua,et al.Effects of seeding manners on lodging index and root growth of directseeded rice[J].,2016,(05):725.[doi:10.3969/j.issn.100-4440.2016.04.002]
[3]易能,薛延丰,石志琦,等.微囊藻毒素对水稻种子萌发和幼苗生长的胁迫作用[J].江苏农业学报,2016,(04):729.[doi:10.3969/j.issn.100-4440.2016.04.003]
　YI Neng,XUE Yan-feng,SHI Zhi-qi,et al.Inhibitory effect of microcystins on seed germination and seedling growth of rice[J].,2016,(05):729.[doi:10.3969/j.issn.100-4440.2016.04.003]
[4]刘凯,王爱民,严国红,等.一个水稻显性矮秆突变体的遗传特性与降株高能力[J].江苏农业学报,2016,(05):968.[doi:10.3969/j.issn.1000-4440.2016.05.002]
　LIU Kai,WANG Ai-min,YAN Guo-hong,et al.Genetic analysis and plant height reduction of a dominant dwarf mutant of rice[J].,2016,(05):968.[doi:10.3969/j.issn.1000-4440.2016.05.002]
[5]王红,杨镇,裴文琪,等.功能性微生物制剂对镉胁迫下水稻生长及生理特性的影响[J].江苏农业学报,2016,(05):974.[doi:10.3969/j.issn.1000-4440.2016.05.003]
　WANG Hong,YANG Zhen,PEI Wen-qi,et al.Growth and physiological characteristics of cadmium-stressed rice influenced by functional microorganism agent[J].,2016,(05):974.[doi:10.3969/j.issn.1000-4440.2016.05.003]
[6]孙玲,单捷,毛良君,等.基于遥感和Moran's I指数的水稻面积变化空间自相关性研究[J].江苏农业学报,2016,(05):1060.[doi:10.3969/j.issn.1000-4440.2016.05.017]
　SUN Ling,SHAN Jie,MAO Liang-jun,et al.Spatial autocorrelation of changes in paddy rice area based on remote sensing and Moran’s I index[J].,2016,(05):1060.[doi:10.3969/j.issn.1000-4440.2016.05.017]
[7]张晓忆,李卫国,景元书,等.多种光谱指标构建决策树的水稻种植面积提取[J].江苏农业学报,2016,(05):1060.[doi:10.3969/j.issn.1000-4440.2016.05.018]
　ZHANG Xiao-yi,LI Wei-guo,JING Yuan-shu,et al.Extraction of paddy rice area by constructing the decision tree with multiple spectral indices[J].,2016,(05):1060.[doi:10.3969/j.issn.1000-4440.2016.05.018]
[8]裔传灯,李玮,王德荣,等.水稻GW5基因的1212-bp Indel变异对粒形的影响[J].江苏农业学报,2016,(06):1201.[doi:doi:10.3969/j.issn.1000-4440.2016.06.001]
　YI Chuan-deng,LI Wei,WANG De-rong,et al.Effect of 1212-bp Indel variation of gene GW5 on rice grain shape[J].,2016,(05):1201.[doi:doi:10.3969/j.issn.1000-4440.2016.06.001]
[9]刘红江,陈虞雯,张岳芳,等.不同播栽方式对水稻叶片光合特性及产量的影响[J].江苏农业学报,2016,(06):1206.[doi:doi:10.3969/j.issn.1000-4440.2016.06.002]
　LIU Hong-jiang,CHEN Yu-wen,ZHANG Yue-fang,et al.Effects of planting pattern on leaf photosynthetic characteristics and yield of rice[J].,2016,(05):1206.[doi:doi:10.3969/j.issn.1000-4440.2016.06.002]
[10]郭保卫,许轲,魏海燕,等.钵苗机插水稻茎秆的抗倒伏能力[J].江苏农业学报,2016,(06):1280.[doi:doi:10.3969/j.issn.1000-4440.2016.06.014]
　GUO Bao-wei,XU Ke,WEI Hai-yan,et al.Culm lodging resistance characteristics of bowl seedling mechanical-transplanting rice[J].,2016,(05):1280.[doi:doi:10.3969/j.issn.1000-4440.2016.06.014]

备注/Memo

备注/Memo:
收稿日期：2017-04-15 基金项目：江苏省农业科技自主创新基金项目[CX(14)5107]；江苏省科技支撑计划项目(BE2015355)；现代农业产业技术体系建设专项(CARS-01-47) 作者简介：胡庆峰(1991-),男，江苏南京人，硕士研究生，主要从事水稻遗传育种研究。（Tel）025-84390317；（E-mail）446899328@qq.com 通讯作者：王才林，（Tel）025-84390307；（E-mail）clwang@jaas.ac.cn
更新日期/Last Update: 2017-11-03