[1]于建宁,陈哲,闫乐艳,等.白消安消减鹅胚内源性原始生殖细胞[J].江苏农业学报,2017,(04):863-867.[doi:doi:10.3969/j.issn.1000-4440.2017.04.021]
 YU Jian-ning,CHEN Zhe,YAN Le-yan,et al.Decrease of goose embryonic primordial germ cells(PGCs) with busulfan[J].,2017,(04):863-867.[doi:doi:10.3969/j.issn.1000-4440.2017.04.021]
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白消安消减鹅胚内源性原始生殖细胞()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年04期
页码:
863-867
栏目:
畜牧兽医·水产养殖
出版日期:
2017-08-30

文章信息/Info

Title:
Decrease of goose embryonic primordial germ cells(PGCs) with busulfan
作者:
于建宁1 陈哲1闫乐艳1宋永宏2朱欢喜1李辉1戴子淳1
(1.江苏省农业科学院畜牧研究所/动物品种改良和繁育重点实验室,江苏南京210014;2.阿联酋总统事务部自然保护管理,阿联酋艾因64634)
Author(s):
YU Jian-ning1CHEN Zhe1YAN Le-yan1SONG Yong-hong2ZHU Huan-xi1LI Hui1DAI Zi-chun1
(1.Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Laboratory of Animal Improvement and Reproduction,Nanjing 210014,China;2.Management of Nature Conservation,Department of President’s Affairs,Al Ain 64634,United Arab Emirates)
关键词:
白消安生殖腺原始生殖细胞
Keywords:
goosebusulfangonadprimordial germ cell
分类号:
S835
DOI:
doi:10.3969/j.issn.1000-4440.2017.04.021
文献标志码:
A
摘要:
降低胚胎内源性原始生殖细胞是提高禽类生殖嵌合体效率的最有效途径,本试验首次研究白消安对鹅胚内源性原始生殖细胞的消减作用。将白消安乳化剂直接注射到鹅种蛋的卵黄内,待鹅胚发育到第8 d时分离生殖腺中的PGCs,并统计PGCs数目。结果显示,糖原染色与免疫荧光染色充分证明从鹅胚生殖腺中成功分离到PGCs;较低浓度(每1颗卵注射150 μg)的白消安能显著降低鹅胚内源性原始生殖细胞,从对照的单胚约1 200个PGCs降低到处理组的80个左右。鹅相对于鸡而言对白消安更敏感,较低浓度即可显著降低鹅内源性PGCs。
Abstract:
Elimination of endogenous embryonic primordial germ cells(PGCs) is regarded as the most effective way to enhance the efficiency of poultry germline chimeras. Busulfan was injected into goose egg yolk directly, and PGCs were isolated from goose gonads and counted when goose embryos developed to 8 d. Periodic acid-schiff (PAS) staining and immunofluorescence staining fully demonstrated that PGCs were successfully isolated from goose gonads.A low concentration (150 μg/eggs) busulfan could significantly reduce goose embryonic endogenous PGCs, the number from about 1 200 in the control down to about 80. Compared with chicken, goose embryos were more sensitive to busulfan and low concentration of busulfan worked well.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2017-02-25 基金项目:江苏省农业科技自主创新基金项目[CX(14) 2068];江苏省自然科学基金项目(BK20150546) 作者简介:于建宁(1980-),女,山东烟台人,博士,副研究员,主要从事家禽生物技术研究。(Tel)025-84390341;(E-mail)jianningyu@jaas.ac.cn
更新日期/Last Update: 2017-09-01