[1]李华山,雷鹏,许宗奇,等.耐盐促生菌Agrobacterium sp. DF-2增强黄瓜幼苗耐盐性的研究[J].江苏农业学报,2017,(03):654-661.[doi:doi:10.3969/j.issn.1000-4440.2017.03.025]
　LI Hua-shan,LEI Peng,XU Zong-qi,et al.Halotolerance in cucumber seedlings enhanced by plant growth-promoting rhizobacterium Agrobacterium sp. DF-2[J].,2017,(03):654-661.[doi:doi:10.3969/j.issn.1000-4440.2017.03.025]
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耐盐促生菌Agrobacterium sp. DF-2增强黄瓜幼苗耐盐性的研究()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年03期
页码:
654-661
栏目:
园艺
出版日期:
2017-06-30

文章信息/Info

Title:
Halotolerance in cucumber seedlings enhanced by plant growth-promoting rhizobacterium Agrobacterium sp. DF-2
作者:
李华山1; 雷鹏1; 许宗奇1; 冯小海1; 徐虹1; 马洪波2
（1.南京工业大学食品与轻工学院，江苏南京211816；2.江苏省农业科学院农业资源与环境研究所，江苏南京210014）
Author(s):
LI Hua-shan1; LEI Peng1; XU Zong-qi1; FENG Xiao-hai1; XU Hong1; MA Hong-bo2
（1.College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China;2.Institute of Agricultural Resource and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China）
关键词:
耐盐促生菌; 耐盐性; ACC脱氨酶; K+/Na+值; 抗氧化酶; 脯氨酸积累
Keywords:
Agrobacterium sp.; salt tolerance; ACC deaminase;  K+/Na+ ratio; antioxidant enzyme activity; proline accumulation
分类号:
S642.2
DOI:
doi:10.3969/j.issn.1000-4440.2017.03.025
文献标志码:
A
摘要:
本研究从盐碱区域植物根际土壤中筛选到1株具有较高ACC脱氨酶活性
[405.17 nmol/（g·h） ]的耐盐促生菌 Agrobacterium sp.DF-2。除ACC脱氨酶活性外，菌株还具有降解不溶磷活性，产生植物生长激素IAA，产胞外多糖等特性。此外，菌株可耐受最高8% NaCl浓度胁迫，证明其可应用到盐碱土壤中。通过盆栽试验研究了在75 mmol/L NaCl 盐胁迫下接种DF-2对黄瓜幼苗的生长的影响以及作用机理。结果表明，与盐胁迫处理相比，接种DF-2处理幼苗株高、根长、生物量积累以及叶绿素含量分别显著增加1508%、3435%、1310%和2258%。同时，DF-2也增强宿主植物对K+的吸收，提高抗氧化酶活性和脯氨酸含量；降低对Na+的吸收和丙二醛含量。菌株DF-2主要通过高效的ACC脱氨酶活性，调控K+/Na+值，增强细胞清除活性氧能力和渗透调节能力等途径增强黄瓜幼苗的耐盐性。因此，耐盐促生菌Agrobacterium sp.DF-2具有作为生物肥料用于盐渍土的潜力。
Abstract:
An Agrobacterium sp.DF-2 with high 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity was isolated from the rhizosphere of saline soil. Apart from producing ACC deaminase, the strain exhibited the plant growth-promoting traits of phosphorus-solubilizing activity and yielded IAA and exopolysaccharide. It could tolerate up to 8% NaCl concentration (w/v) stress, indicating its potential for applications to saline soil. The effects of DF-2 inoculation on the growth and physiological responses of cucumber seedlings under salt stress and the mechanism were investigated by pot experiment. Compared to control plants under salt stress, inoculation with DF-2 significantly increased shoot, root and plant biomass and chlorophyll content by 1508%, 3435%, 1310% and 2258%, respectively. Moreover, DF-2 boosted the uptake of K+, antioxidant enzyme activity and proline content, and reduced the uptake of Na+ and malondialdehyde content in the host plant. In conclusion, DF-2 enhanced cucumber seedlings salt tolerance through superior ACC deaminase activity, regulation of K+/Na+ ratio, enhancement of ROS scavenging capacity and an osmotic adjustment ability of cucumber cells. The halotolerant Agrobacterium sp.DF-2 offers great potential for the use as a biological fertilizer in saline soil agriculture to advance sustainable development.

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备注/Memo

备注/Memo:
收稿日期：2017-01-12 基金项目：“十二五”国家科技支撑计划重点项目（2015BAD15B04）；国家高技术研究发展计划（“863”计划）项目（2015AA020951）；国家自然科学基金青年基金项目（21506098）；江苏省高校自然科学研究面上项目（15KJB530007） 作者简介：李华山(1990-)，男，河南汤阴人，硕士研究生，主要从事耐盐促生菌的筛选及微生物菌剂开发等研究。(E-mail) huashan@njtech.edu.cn 通讯作者：冯小海，(E-mail) fengxiaohai@njtech.edu.cn；许宗奇，(E-mail) zqxu@njtech.edu.cn
更新日期/Last Update: 2017-06-29