[1]李鹏成,杨倩,侯继波.乳酸杆菌S-层蛋白对产肠毒素大肠杆菌黏附Caco-2细胞的协同作用[J].江苏农业学报,2017,(02):384-388.[doi:doi:10.3969/j.issn.1000-4440.2017.02.023]
　LI Peng-cheng,YANG Qian,HOU Ji-bo.Influence of S-layer proteins from Lactobacillus on enterotoxigenic Escherichia coli adhesion to Caco-2 cells[J].,2017,(02):384-388.[doi:doi:10.3969/j.issn.1000-4440.2017.02.023]
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乳酸杆菌S-层蛋白对产肠毒素大肠杆菌黏附Caco-2细胞的协同作用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年02期
页码:
384-388
栏目:
畜牧兽医·水产养殖
出版日期:
2017-04-30

文章信息/Info

Title:
Influence of S-layer proteins from Lactobacillus on enterotoxigenic Escherichia coli adhesion to Caco-2 cells
作者:
李鹏成1; 杨倩2; 侯继波1
（1.江苏省农业科学院国家兽用生物制品工程技术研究中心，江苏南京210014；2.南京农业大学动物医学院，江苏南京210095）
Author(s):
LI Peng-cheng1; YANG Qian2; HOU Ji-bo1
(1.National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Science, Nanjing 210014, China；2.College of Veterinary, Nanjing Agricultural University, Nanjing 210095, China)
关键词:
嗜酸乳酸杆菌; S-层蛋白; 产肠毒素大肠杆菌; Caco-2细胞; 协同作用
Keywords:
Lactobacillus acidophilus; S-layer protein; enterotoxigenic E.coli; Caco-2 cells; synergistic effect
分类号:
S852.61+5
DOI:
doi:10.3969/j.issn.1000-4440.2017.02.023
文献标志码:
A
摘要:
设计3种黏附试验分别研究了嗜酸乳酸杆菌和S-层蛋白对产肠毒素大肠杆菌黏附Caco-2细胞的影响。结果显示：在置换试验中，嗜酸乳酸杆菌及S-层蛋白对产肠毒素大肠杆菌黏附Caco-2细胞没有影响；但在排斥试验和竞争试验中，嗜酸乳酸杆菌及S-层蛋白均对产肠毒素大肠杆菌黏附Caco-2细胞产生明显的协同作用，在排斥试验中嗜酸乳酸杆菌及S-层蛋白分别增加产肠毒素大肠杆菌的黏附数量 95.23%±4.22%（P＜0.01）和 352.30%±2.26%（P＜0.01），在竞争试验中分别增加 389.06%±3.35%（P＜0.01）和 55.57%±5.81%（P＜0.05）；并且S-层蛋白在排斥试验中，对产肠毒素大肠杆菌的协同黏附作用大于竞争试验中的协同黏附作用。可见，乳酸杆菌对产肠毒素大肠杆菌的协同黏附作用关键在于S-层蛋白，S-层蛋白可能起到“连接桥”的作用。
Abstract:
Three adhesive experiments were designed to investigate the effects of Lactobacillus acidophilus and its S-layer proteins on the adhesion of enterotoxigenic E. coli to Caco-2 cells. The results showed that S-layer proteins exhibited strongly synergistic effect of adhesive properties of enterotoxigenic E. coli in the exclusive and competitive experiments but not in the displacement experiment. In the exclusive experiments, Lactobacillus acidophilus and its S-layer proteins increased the number of enterotoxigenic E. coli adhered to Caco-2 cells by 95.23%±4.22% (P＜0.01) and 352.30%±2.26% (P＜0.01). In the competitive experiments, Lactobacillus acidophilus and its S-layer proteins increased the number of enterotoxigenic E. coli adhered to Caco-2 cells by 389.06%±3.35% (P＜0.01) and 55.57%±5.81% (P＜0.05). Furthermore, the S-layer proteins showed a stronger effect in the exclusive experiment than that in the competitive experiment to increase enterotoxigenic E. coli adhesion to Caco-2 monolayers. The data indicat that S-layer proteins play a critical role on the synergistic effect of Lactobacillus acidophilus on the adherence ability of enterotoxigenic E.coli, which may be relative to the S-layer proteins-induced bridge.

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备注/Memo

备注/Memo:
收稿日期：2016-05-19 基金项目：国家自然科学基金项目(31502105) 作者简介：李鹏成（1981-），男，山西文水人，博士，助理研究员，从事兽用生物制品工程技术研究。（E-mail）lipengcheng305@163.com 通讯作者：杨倩，（E-mail）zxbyq@njau.edu.cn；侯继波，（E-mail）houjibo@jaas.ac.cn
更新日期/Last Update: 2017-05-02