[1]胡雪丹,张曼,侯茜,等.不同影响因子诱导葫芦花药愈伤组织[J].江苏农业学报,2016,(05):1155-1161.[doi:10.3969/j.issn.1000-4440.2016.05.032]
 HU Xue-dan,ZHANG Man,HOU Qian,et al.Induction of calli in anther culture of gourd influenced by temperature, hormone and activated carbon[J].,2016,(05):1155-1161.[doi:10.3969/j.issn.1000-4440.2016.05.032]
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不同影响因子诱导葫芦花药愈伤组织()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年05期
页码:
1155-1161
栏目:
园艺
出版日期:
2016-11-22

文章信息/Info

Title:
Induction of calli in anther culture of gourd influenced by temperature, hormone and activated carbon
作者:
胡雪丹12张曼1侯茜1徐锦华1刘广1姚协丰1李苹芳1任润生1陈学好2羊杏平1
1. 江苏省农业科学院蔬菜研究所/江苏省高效园艺作物遗传改良重点实验室,江苏南京210014;2. 扬州大学园艺与植物保护学院,江苏扬州225009
Author(s):
HU Xue-dan12ZHANG Man1HOU Qian1XU Jin-hua1LIU Guang1YAO Xie-feng1LI Ping-fang1REN Run-sheng1CHEN Xue-hao2YANG Xing-ping1
1. Institute of Vegetable, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China;2. College of Horticulture and Plant Protection,Yangzhou University, Yangzhou 225009, China
关键词:
葫芦花药培养愈伤组织
Keywords:
gourdanther culturecallus
分类号:
S642.9
DOI:
10.3969/j.issn.1000-4440.2016.05.032
文献标志码:
A
摘要:
为研究葫芦花药愈伤组织诱导的影响因素,筛选出最适宜诱导葫芦花药愈伤组织的培养条件,以3个葫芦品种为试验材料,观察不同低温预处理、热激处理以及激素和活性炭处理对花药愈伤组织诱导率及生长的影响。结果表明,不同基因型愈伤组织诱导率差异显著;最佳预处理方法是4 ℃低温预处理2 d,33 ℃高温热激暗培养1 d;MS培养基添加2,4-D和6-BA能诱导出愈伤组织,愈伤组织按外观和质地分为6种类型,随着激素浓度增加愈伤组织诱导率递增,添加1.5 mg/L 2,4-D+2.0 mg/L 6-BA和2.0 mg/L 2,4-D+2.0 mg/L 6-BA的培养基最有利于诱导愈伤组织;MS基本培养基不能诱导愈伤组织,活性炭抑制花药诱导愈伤组织。说明,在添加了适量浓度的2,4-D和6-BA的MS培养基上能成功诱导出葫芦花药愈伤组织。
Abstract:
The factors affecting the induction of anther callus were studied on three gourd varieties. The induction rate of calli varied in different gourd genotypes. The best pretreatment was set at 4 ℃ for two days and dark culture at 33 ℃ for one day. MS medium added with 2,4-D and 6-BA could induce calli which were divided into 6 types according to the appearance and texture. With the increase of hormone concentrations, the induction rate of calli increased. MS basic medium added with 1.5 mg/L 2,4-D+2.0 mg/L 6-BA and 2.0 mg/L 2,4-D+2.0 mg/L 6-BA were favorable for callus induction. Activated carbon could inhibit the induction of callus.

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备注/Memo

备注/Memo:
收稿日期:2016-02-01 基金项目:国家科技支撑计划项目(2012BAD02B03-14);国家西甜瓜产业技术体系岗位科学家项目(CARS-NO.26);江苏省农业科技自主创新基金项目[CX(14)2004] 作者简介:胡雪丹(1990-),女,江苏泰州人,硕士研究生,研究方向为葫芦花药培养及游离小孢子培养。(E-mail) 276518157@qq.com 通讯作者:羊杏平,(E-mail)xingping@jaas.ac.cn
更新日期/Last Update: 2016-11-22