[1]巫建华,贾思振,冯英娜,等.茉莉酸合成关键酶基因FaOPR3调控草莓果实成熟[J].江苏农业学报,2016,(05):1148-1154.[doi:10.3969/j.issn.1000-4440.2016.05.031]
　WU Jian-hua,JIA Si-zhen,FENG Ying-na,et al.Regulation of strawberry fruit ripening by jasmonic acid synthesis gene FaOPR3[J].,2016,(05):1148-1154.[doi:10.3969/j.issn.1000-4440.2016.05.031]
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茉莉酸合成关键酶基因FaOPR3调控草莓果实成熟()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年05期
页码:
1148-1154
栏目:
园艺
出版日期:
2016-11-22

文章信息/Info

Title:
Regulation of strawberry fruit ripening by jasmonic acid synthesis gene FaOPR3
作者:
巫建华12; 贾思振12; 冯英娜12; 王全智12
1. 江苏农林职业技术学院，江苏镇江212400；2. 江苏现代园艺工程技术中心，江苏镇江212400
Author(s):
WU Jian-hua12; JIA Si-zhen12; FENG Ying-na12; WANG Quan-zhi12
1. Jiangsu Polytechnic College of Agriculture and Forestry, Zhenjiang 212400，China;2. Jiangsu Engineering and Technology Center for Modern Horticulture，Zhenjiang 212400，China
关键词:
茉莉酸; 草莓; FaOPR3基因; 果实着色; 果实成熟
Keywords:
jasmonic acid; strawberry; FaOPR3 gene; fruit coloring; fruit ripening
分类号:
S663.901
DOI:
10.3969/j.issn.1000-4440.2016.05.031
文献标志码:
A
摘要:
为了研究茉莉酸（Jasmonic acid, JA）合成路径关键酶基因FaOPR3在草莓果实成熟中的作用，测定了八倍体红颜草莓果实成熟过程中内源JA含量，并用外源茉莉酸甲酯（MeJA）涂抹草莓果实观察其果实着色情况及测定相关酶基因的表达，同时使用瞬时基因表达对草莓果实进行FaOPR3基因的超表达和干扰。结果显示草莓果实中内源JA含量从小绿果期到白果期急剧上升，到果实成熟后开始下降。草莓果实涂抹外源JA，能够促进果实发育和成熟。FaOPR3基因的过量表达可诱导果实内源JA含量的增加，并且可诱导色素合成基因FaCHS、FaCHI、FaF3H、FaUFGT、FaDFR的表达量上升。而FaOPR3基因干扰后果实内源JA含量降低，并且抑制相关色素代谢基因表达。说明FaOPR3基因能够促进草莓果实着色和成熟。
Abstract:
To study the role of jasmonic acid (JA) synthesis key enzyme gene FaOPR3 in strawberry fruit development, the endogenous JA content in the octoploid strawberry Benihoppe (Fragaria×ananassa Duch.) during fruit ripening were measured ,the fruit coloring was observed and related genes expression levels were detected. The endogenous JA content increased dramatically from small green fruit period to white fruit period, peaked at white fruit period, and dropped quickly afterwards. Exogenous MeJA treatment promoted strawberry fruit development and ripening. Transient gene expression revealed that FaOPR3 over-expression induced the increase of endogenous JA content in fruit and pigment metabolism-associated genes expression levels, such as FaCHS,FaCHI,FaF3H,FaUFGT and FaDFR genes. On the contrary, FaOPR3 gene interference reduced JA content and inhibited pigment metabolism-related genes expressions. It is indicated that FaOPR3 gene plays an important role in strawberry fruit ripening, promoting strawberry fruit coloring and ripening.

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备注/Memo

备注/Memo:
收稿日期：2016-01-23 基金项目：江苏省农业三新工程项目[SXGC(2014)276、SXGC(2015)311、SXGC(2015)312]; 江苏省自然科学基金项目（BK20131243） 作者简介：巫建华（1965-）,男，博士，安徽广德人，推广研究员，主要从事园艺生产和园艺植物分子生物学研究。(E-mail）932465017@qq.com
更新日期/Last Update: 2016-11-22