[1]陆吉虎,吴培培,张雪花,等.免疫增强剂提高H9亚型禽流感灭活疫苗在SPF鸡体中细胞因子的免疫应答[J].江苏农业学报,2016,(02):408-413.[doi:10.3969/j.issn.1000-4440.2016.02.026]
　LU Ji-hu,WU Pei-pei,ZHANG Xue-hua,et al.Immunopotentiators improving the cytokine immune response to inactivated H9 subtype avian influenza vaccine in SPF chickens[J].,2016,(02):408-413.[doi:10.3969/j.issn.1000-4440.2016.02.026]
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免疫增强剂提高H9亚型禽流感灭活疫苗在SPF鸡体中细胞因子的免疫应答()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年02期
页码:
408-413
栏目:
畜牧兽医·水产养殖
出版日期:
2016-03-20

文章信息/Info

Title:
Immunopotentiators improving the cytokine immune response to inactivated H9 subtype avian influenza vaccine in SPF chickens
作者:
陆吉虎1; 2; 3;  吴培培1; 3;  张雪花1; 2; 3;  侯继波1; 3;  唐应华1; 3
1.江苏省农业科学院国家兽用生物制品工程技术研究中心/农业部兽用生物制品工程技术重点实验室,江苏 南京 210014; 2.南京天邦生物科技有限公司,江苏 南京 211102; 3. 江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏 扬州 225009
Author(s):
LU Ji-hu1; 2; 3;  WU Pei-pei1; 3;  ZHANG Xue-hua1; 2; 3;  HOU Ji-bo1; 3;  TANG Ying-hua1; 3
1.National Research Center of Engineering and Technology for Veterinary Biologicals/Key Laboratory of Engineering and Technology for Veterinary Blologicals, Ministry of Agriculture,Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2.Nanjing Tianbang Bio-industry Co., Ltd., Nanjing 211102, China; 3.Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China
关键词:
免疫增强剂;  CD4+ T淋巴细胞;  CD8+T淋巴细胞;  细胞因子;  血凝抑制抗体
Keywords:
immunopotentiators;  CD4+ T lymphocytes;  CD8+ T lymphocytes;  cytokine;  antibody of hemagglutinin inhibition
分类号:
S831.7
DOI:
10.3969/j.issn.1000-4440.2016.02.026
文献标志码:
A
摘要:
为探讨免疫增强剂(VA5)提高SPF鸡免疫功能的机理,将SPF鸡分别免疫H9亚型禽流感灭活疫苗、含VA5 的H9灭活疫苗、含IL-4和/或IFN-γ真核质粒的H9亚型禽流感灭活疫苗,另设空白对照。检测免疫鸡外周血CD4+、CD8+T淋巴细胞亚群数目及IL-4和IFN-γ细胞因子含量的变化及血清抗体效价。结果显示,在14日龄、17日龄和21日龄和28日龄,免疫IL-4和H9、IL-4与IFN-γ和H9以及VA5佐剂与H9组的CD4+淋巴细胞亚群上升快且比例高于其他组,免疫IFN-γ和H9、IL-4与IFN-γ和H9以及VA5增强剂与H9组的CD8+淋巴细胞亚群上升快且比例高于其他组,IL-4的上升趋势与CD4+细胞类似,IFN-γ的上升趋势与CD8+类似。免疫含VA5的禽流感(H9亚型)灭活苗组在免疫后第2周、3周和4周血清HI效价达到7lg2以上,高于其他各免疫组。说明,与直接添加IL-4和IFN-γ的细胞因子质粒组相比,VA5免疫增强剂能够促进SPF鸡外周血淋巴细胞增值,提高CD4+、CD8+T淋巴细胞的比例,并诱导IL-4和IFN-γ细胞因子分泌。
Abstract:
The aim of this study is to explore the immunological mechanism of immunopotentiator VA5 in improving the immune response of specific pathogen-free(SPF)chickens. SPF chickens were challenged with the inactivated H9 subtype vaccine, VA5+H9 vaccine, IL-4+H9 vaccine, IFN-γ+H9 vaccine, IL-4+IFN-γ+H9 vaccine, and blank control, respectively. The number of CD4+ or CD8+ subgroup lymphocytes from peripheral blood mononuclear cells, the contents of IL-4 and IFN-γ from sera and the antibody levels of hemagglutinin inhibition(HI)were detected. The number of CD4+ subgroup lymphocytes from chickens in groups of IL-4+H9, IL-4+IFN-γ+H9, and VA5+H9 climbed faster and the percentage was higher than those in other groups at the 14-, 17-, 21- and 28-day ages. The number of CD8+ subgroup lymphocytes from chickens in groups of IFN-γ+H9, IL-4 and IFN-γ+H9 vaccine and VA5+H9 vaccine climbedfaster and the percentage was higher than those in other groups IL4 showed similar rising trend to the CD4+ subgroup lymphocytes and IFN-γ presented similar rising trend to the CD8+ subgroup lymphocytes. The HI antibody levels of chickens in group of VA5+H9 were 7lg2 at 2-, 3-and 4-week postvaccination, higher than those in other groups. In summary, the immunopotentiator VA5 increased the percantages of CD4+ and CD8+ subgroup lymphocytes and induced high secretions of IL-4 and IFN-γ.

参考文献/References:

[1] NIU X, YANG Y, WANG J. Synergistic and additive effects of cimetidine and levamisole on cellular immune responses to hepatitis B virus DNA vaccine in mice[J]. Scand J Immunol,2013,77(2):84-91.
[2] ZHANG Y, CHEN H, ZENG X, et al. Levamisole enhances immunity in ducklings vaccinated against Riemerella anatipestifer[J]. Microbiol Immunol, 2014, 58(8):456-462.
[3] LUNDBERG A M, DREXLER S K, MONACO C,et al. Key differences in TLR3/Poly I: C signaling and cytokine induction by human primary cells: a phenomenon absent from murine cell systems[J]. Blood, 2007, 110(9): 3245-3252.
[4] MALLICK A I, PARVIZI P, READ L R, et al. Enhancement of immunogenicity of a virosome-based avian influenza vaccine in chickens by incorporating CpG-ODN[J]. Vaccine, 2011, 29(8): 1657-1665.
[5] LATZ E, VISINTIN A, LIEN E, et al. The LPS receptor generates inflammatory signals from the cell surface[J]. J Endotoxin Res, 2003, 9(6): 375-380.
[6] YANG X, QIAN F, HE H Y, et al. Effect of thymosin alpha-1 on subpopulationa of Th1, Th2, Th17, and regulatory T cells(Tregs)in vitro[J]. Braz J Med Biol Res, 2012, 45(1):25-32.
[7] LI D Y, XUE M Y, WANG C, et al. Bursopentine as a Novel immunoadjuvant enhances both humoral and cell-mediated immune responses to inactivated H9N2 avian influenza virus in chickens[J]. Clinl Vaccine Immunol, 2011, 18(9): 1497-1502.
[8] 雷爱莹,江林源. 蜈蚣藻多糖对凡纳滨对虾免疫相关基因表达的影响[J]. 南方农业学报,2014,45(4):682-686.
[9] 唐应华,陆吉虎,吴培培,等. 免疫增强剂提高禽流感疫苗效力的研究[J].江苏农业学报,2014,30(2):344-348.
[10] 唐应华,陆吉虎,吴培培,等. 免疫增强剂对禽流感疫苗免疫持续期、安全性和鸡淋巴细胞转化的影响[J].江苏农业学报,2014,30(4):821-825.
[11] TANG Y H, LU J H, WU P P, et al. Inactivated vaccine with adjuvants consisting of pattern recognition receptor agonists confers protection against avian influenza viruses in chickens[J]. Vet Microbiol, 2014, 172(1-2):120-128.
[12] PHARES T W, STOHLMAN S A, HWANG M. CD4 T cells promote CD8 T cell immunity at the priming and effector site during viral encephalitis[J]. J Virol, 2012, 86(5):2416-2427.
[13] KAMALAVENKATESH P, VAIRAMUTHU S, BALACHANDRAN C. Immunopathological effect of the mycotoxins cyclopiazonic acid and T-2 toxin on broiler chicken[J]. Mycopathologia, 2005, 159(2): 273-279.
[14] DIAZ-ROMERO J, VOGT G, WECKBECKER G. Coexpression of CD4 and CD8alpha on rat T-cells in whole blood: a sensitive marker for monitoring T-cell immunosuppressive drugs[J]. J Immunol Methods, 2001, 254(1-2):1-12.
[15] AHMED M, VENKATARAMAN R, LOGAR A J, et al. Quantitation of immunosuppresion by tacrolimus using flow cytometric analysis of interleukin-2 and interferon- gamma inhibition in CD8- and CD8+ peripheral blood T cells[J]. Ther Drug Monit, 2001, 23(4): 354-362.
[16] IWASAKI A,MEDZHITOV R. Egulation of adaptive immunity by the innate immune system[J]. Science, 2010, 327(5963):291-295.
[17] SAWANT P M, VERMA P C, SUBUDHI P K, et al. Immunomodulation of bivalent Newcastle disease DNA vaccine induced immune response by co-delivery of chicken IFN-γ and IL-4 genes[J]. Vet Immunol Immunopathol, 2011, 144(1-2):36-44.

备注/Memo

备注/Memo:
收稿日期:2015-11-24 基金项目:国家自然科学基金项目(31201904); 农业部公益性行业(农业)科研专项(201303046); 中央财政农业技术推广资金项目[TG(14)067]; 江苏省农业科技自主创新基金项目[CX(14)5045] 作者简介:陆吉虎(1972-),男,江苏赣榆人,硕士,助理研究员,主要从事兽医微生物与免疫学研究。(E-mail)lujihu@163.com 通讯作者:唐应华,(E-mail)tyinghua@yahoo.com
更新日期/Last Update: 2016-03-20