[1]锡林高娃,薛晓阳,吴金花,等.金黄色葡萄球菌表面蛋白nEBPS抗体的制备和纯化[J].江苏农业学报,2015,(06):1430-1434.[doi:doi:10.3969/j.issn.1000-4440.2015.06.037]
 XI LIN Gao-wa,XUE Xiao-yang,WU Jin-hua,et al.Preparation and purification of polyclonal antibody against Staphylococcus aureus surface protein nEBPS[J].,2015,(06):1430-1434.[doi:doi:10.3969/j.issn.1000-4440.2015.06.037]
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金黄色葡萄球菌表面蛋白nEBPS抗体的制备和纯化()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年06期
页码:
1430-1434
栏目:
加工贮藏·质量安全
出版日期:
2015-12-31

文章信息/Info

Title:
Preparation and purification of polyclonal antibody against Staphylococcus aureus surface protein nEBPS
作者:
锡林高娃12薛晓阳12吴金花12布日额12
(1.内蒙古民族大学生命科学学院,内蒙古通辽028400;2.内蒙古民族大学乳源性致病菌研究所,内蒙古通辽028400)
Author(s):
XI LIN Gao-wa12XUE Xiao-yang12WU Jin-hua12BU Ri-e12
(1.College of Life Sciences, Inner Mongolia University for the Nationalities, Tongliao 028000, China;2.Research Institute of Pathogens in Milk, Inner Mongolia University for Nationalities, Tongliao 028000, China)
关键词:
nEBPS抗体多克隆抗体制备纯化
Keywords:
nEBPSpolyclonal antibodypreprationpurification
分类号:
S852.61+1
DOI:
doi:10.3969/j.issn.1000-4440.2015.06.037
文献标志码:
A
摘要:
为了在原核细胞中表达金黄色葡萄球菌弹性纤维结合蛋白(EBPS),制备多克隆抗体,将构建的pET30a-nEBPS重组质粒转化大肠杆菌BL21 (DE3),经IPTG诱导表达后,亲和层析法纯化重组蛋白,以纯化的重组蛋白为抗原免疫新西兰大耳白兔制备多克隆抗体,ELISA方法检侧抗体滴度,亲和层析法纯化兔抗nEBPS多克隆抗体。结果显示,利用pET30a-nEBPS重组质粒,经原核表达和亲和层析获得了nEBPS蛋白;利用nEBPS蛋白质制备多克隆抗体,间接ELISA检测的纯化抗体效价可达0.686(OD450值),P/N值达6.7。
Abstract:
To prepare polyclonal antibody of Staphylococcus aureus surface protein nEBPS, the plasmid pET30a-nEBPS was transformed into Escherichia coli BL21 (DE3). IPTG induction were performed and recombinant protein was purified by nickel affinity chromatography. The purified nEBPS protein was used to raise rabbit nEBPS protein antibody. The enzyme-linked immunosorbent assay (ELISA) showed that the antibody titers against nEBPS was up to 0.686 (OD450), with P/N value of 6.7.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-07-06 基金项目:国家自然科学基金项目(31260600);内蒙古自治区科技厅科技创新引导计划项目
[20111802(2012年、2013年)];内蒙古自治区自然科学基金项目(2012MS0415) 作者简介:锡林高娃(1976-),女,内蒙古锡林浩特市人,硕士,讲师,主要从事病原微生物及细胞工程研究。(E-mail)xilingaowa123@163.com 通讯作者:布日额,(E-mail)wjhbre@yahoo.com.cn;wjhbre@aliyun.com
更新日期/Last Update: 2015-12-31