[1]贺江,龙海波.分散液相微萃取-高效液相色谱法测定水体中毒死蜱残留[J].江苏农业学报,2015,(05):1179-1183.[doi:doi:10.3969/j.issn.1000-4440.2015.05.037]
　HE Jiang,LONG Hai-bo.A dispersive liquid-liquid microextraction-HPLC method for determination of chlorpyrifos in water[J].,2015,(05):1179-1183.[doi:doi:10.3969/j.issn.1000-4440.2015.05.037]
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分散液相微萃取-高效液相色谱法测定水体中毒死蜱残留()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年05期
页码:
1179-1183
栏目:
加工贮藏·质量安全
出版日期:
2015-10-31

文章信息/Info

Title:
A dispersive liquid-liquid microextraction-HPLC method for determination of chlorpyrifos in water
作者:
贺江; 龙海波
（湖南文理学院 生命科学学院, 环洞庭湖水产健康养殖与加工湖南省重点实验室, 湖南省水产高效健康生产协同创新中心, 湖南常德415000）
Author(s):
HE Jiang; LONG Hai-bo
(College of Life Science, Hunan University of Art and Science/Hunan Provincial Key Laboratory of Aquatic Healthy Farming and Processing in Dongting Lake/ Collaborative Innovation Center for Efficient and Healthy Production of Fisheries in Hunan Province, Changde 415000,China)
关键词:
分散液相微萃取; 高效液相色谱; 毒死蜱; 水体; 农药残留
Keywords:
dispersive liquid-liquid micro extraction; high performance liquid chromatography; chlorpyrifos; water; pesticide residue
分类号:
S132
DOI:
doi:10.3969/j.issn.1000-4440.2015.05.037
文献标志码:
A
摘要:
水体中的农药残留是严重的环境安全问题之一，并可通过水产养殖或种植业等途径污染食品。本研究应用分散液相微萃取（Dispersive liquid-liquid microextraction ,DLLME）技术，建立水体中毒死蜱的高效液相色谱（High performance liquid chromatography ,HPLC）分析方法。通过考察萃取剂种类、分散剂种类、萃取剂用量、分散剂用量、盐度以及pH等因素对分散液相微萃取的影响，确定最佳操作参数。经优化的水体中毒死蜱分散液相微萃取过程为：以100 μl三氯乙烷为萃取剂，1 200 μl甲醇为分散剂，混匀后，加入10 ml水样，室温静置2 min，以3 000 r/min离心2 min，吸取15 μl沉积相，进行HPLC分析。本方法操作简单、成本低，灵敏度、准确度及精密度高，可为水体中毒死蜱残留检测提供新的方法。
Abstract:
The residue of pesticides in water system is one of the serious environmental safety problems, and may contaminate food through a variety of ways. In the current study, dispersive liquid-liquid micro-extraction (DLLME) technology was applied for develop a high performance liquid chromatography (HPLC) analysis methods for the detection of chlorpyrifos in water. In the optimized protocol, the mixture of 100 μl trichloroethane used as extract agent and 1 200 μl methanol used as dispersing agent were added with 10.0 ml water samples and stored at room temperature for 2 min, and 15 μl deposition sampled from above mixture centrifuged at 3 000  r/min for 2 min were subjected to HPLC analysis. The newly developed method was applied to detected the chlorpyrifos with the concentrations ranging from 0.01~1.00 mg/L, the results showed the spiked recoveryes were above 85.5%, indicative of the sensitivity, accuracy and precision. This method offers a new approch for the detection of chlorpyrifos in water sample.〖JP〗

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备注/Memo

备注/Memo:
收稿日期：2015-03-20 基金项目：国家自然科学基金项目（31401583）；湖南省教育厅科研项目（12C0828）；湖南省常德市联合基金项目（2015JJ5008）；湖南文理学院生命科学学院省级平台开放项目（SKYPT201202） 作者简介：贺江（1983-），男，江西萍乡人，博士，讲师，主要从事食品安全与食品生物技术方面的研究。(Tel)15115667084;(E-mail) hejiang1119@163.com
更新日期/Last Update: 2015-10-31