[1]蒋春茂,陈晓兰,陆广富,等.不同中药多糖体外对鸡外周血和脾脏淋巴细胞增殖能力的比较[J].江苏农业学报,2015,(01):105-111.[doi:10.3969/j.issn.1000-4440.2015.01.016]
　JIANG Chun-mao,CHEN Xiao-lan,LU Guang-fu,et al.Comparative study on chicken peripheral lymphocyte and spleen lymphocyte proliferation by Chinese herbal medicinal polysaccharides in vitro[J].,2015,(01):105-111.[doi:10.3969/j.issn.1000-4440.2015.01.016]
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不同中药多糖体外对鸡外周血和脾脏淋巴细胞增殖能力的比较()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年01期
页码:
105-111
栏目:
畜牧兽医·水产养殖
出版日期:
2015-02-28

文章信息/Info

Title:
Comparative study on chicken peripheral lymphocyte and spleen lymphocyte proliferation by Chinese herbal medicinal polysaccharides in vitro
作者:
蒋春茂1; 陈晓兰12; 陆广富1; 陈兴颖2; 王德云2; 胡元亮2; 耿千丽1
（1.江苏农牧科技职业学院,江苏泰州225300；2.南京农业大学动物医学院,江苏南京2100951）
Author(s):
JIANG Chun-mao1; CHEN Xiao-lan12; LU Guang-fu1; CHEN Xing-ying2; WANG De-yun2 ; HU Yuan-liang2; GEN Qian-li1
（1.Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China;2.College of Veterinary Medicine，Nanjing Agricultural University ,Nanjing 210095, China）
关键词:
中药多糖; 淋巴细胞增殖; 植物血凝素（PHA）; 脂多糖（LPS）
Keywords:
Chinese herbal medicinal polysaccharide; lymphocyte proliferation; phytohaemagglutinin(PHA); lipopolysaccharide (LPS)
分类号:
S853.74
DOI:
10.3969/j.issn.1000-4440.2015.01.016
文献标志码:
A
摘要:
为比较玉竹多糖、桑叶多糖、杜仲多糖、商陆多糖、淫羊藿多糖体外增强免疫活性，采用酶学检测法测定各中药多糖对鸡胚成纤维细胞的安全浓度，根据安全浓度测定结果，选择250.000 μg/ml、125.000 μg/ml、62.500 μg/ml、31.250 μg/ml、15.625 μg/ml 5个浓度，用MTT法比较5种中药多糖单独和PHA协同刺激对外周血T淋巴细胞增殖指数的影响，以及比较5种中药多糖单独和LPS协同刺激对脾脏B淋巴细胞增殖率的影响。结果显示， 玉竹多糖、桑叶多糖、杜仲多糖、商陆多糖、淫羊藿多糖对鸡胚成纤维细胞的安全浓度分别为1 250.0 μg/ml、625.0 μg/ml、625.0 μg/ml、625.0 μg/ml、312.5 μg/ml。玉竹多糖、桑叶多糖、杜仲多糖和淫羊藿多糖无论是单独还是协同PHA刺激外周血T淋巴细胞，均不同程度地表现出增强T淋巴细胞的活性。桑叶多糖、杜仲多糖和淫羊藿多糖无论是单独还是协同LPS刺激脾脏B淋巴细胞，均不同程度地显示出良好的增强B淋巴细胞的活性。桑叶多糖和杜仲多糖体外增强免疫效果最佳，在62.500 μg/ml和125.000 μg/ml时，均显示出较强的增强外周血T淋巴细胞和脾脏B淋巴细胞增殖的能力。
Abstract:
Polygonatum odoratum polysaccharide (POPS), morus polysaccharide(MPS), eucommia polysaccharide(EPS), pokeweed polysaccharide (PPS), and epimedium polysaccharide(EPPS) to chicken embryonic fibroblast(CEF) in vitro were determined and showed as follows compared. 1 250.0 μg/ml， 625.0 μg/ml， 625.0 μg/ml， 625.0 μg/ml， and 312.5 μg/ml， respectively. The effects of above five polysaccharides applied solely or co-applied with phytohaema glutinin(PHA) on the proliferation of peripheral blood T lymphocytes and the effects of five paly saccharides appiied solely or co-applied with lipopolysaccharide LPS on the proliferation of spleenic B lymphocytes were compared. POPS, MPS, EPS and EPPS could enhance peripheral blood Tlymphocytes activity at different levels either stimulated alone or co-stimulated with PHA. MPS, EPS and EPPS could improve spleenic B lymphocytes activity at different levels either stimulated alone or co-stimulated with LPS. The immune enhancements of MPS and EPS in vitro outperformed others at the concentration of 62.5 μg/ml and 125 μg/ml.

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备注/Memo

备注/Memo:
收稿日期：2014-06-20 基金项目：江苏省自然科学基金面上项目（BK2011537）；江苏省农业三新工程项目[SXGC(2013)291]；江苏农牧科技职业学院凤凰人才专项（NSFFH1303）；江苏省大学生实践创新项目（201412806016Y）；学院重点项目（NSFZD1404） 作者简介：蒋春茂（1962-），男，江苏靖江人，博士，教授，主要从事新兽药研发。
更新日期/Last Update: 2015-02-28