[1]于建宁,杨燕燕,于峰祥,等.硫化物醌氧化还原酶定向表达载体的构建及细胞转染表达检测[J].江苏农业学报,2015,(01):100-104.[doi:10.3969/j.issn.1000-4440.2015.01.015]
　YU Jian-ning,YANG Yan-yan,YU Feng-xiang,et al.Construction of directioned expression vector for sulfide-quinone reductase gene and its expression[J].,2015,(01):100-104.[doi:10.3969/j.issn.1000-4440.2015.01.015]
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硫化物醌氧化还原酶定向表达载体的构建及细胞转染表达检测()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年01期
页码:
100-104
栏目:
畜牧兽医·水产养殖
出版日期:
2015-02-28

文章信息/Info

Title:
Construction of directioned expression vector for sulfide-quinone reductase gene and its expression
作者:
于建宁1; 杨燕燕2; 于峰祥3; 时小艳4; 徐小波1; 王公金1
（1.江苏省农业科学院畜牧研究所/动物品种改良和繁育重点实验室，江苏南京210014；2.青岛市植物园管理处，山东青岛266071；3.南京农业大学动物科技学院，江苏南京210095；4.江苏食品职业技术学院医药与健康管理系，江苏淮安223003）
Author(s):
YU Jian-ning1; YANG Yan-yan2; YU Feng-xiang3; SHI Xiao-yan4; XU Xiao-bo1; WANG Gong-jin1
（1.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences/Laboratory of Animal Improvement and Reproduction, Nanjing 210014,China;2.Qingdao Botanical Gardens Management Office, Qingdao 266071， China;3.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;4.Department of Medical and Health Management, Jiangsu Food Science College, Huai′an 223003, China）
关键词:
硫化物醌氧化还原酶; 定向表达载体; 肠脂肪酸结合蛋白质; 肠上皮细胞
Keywords:
sulfide-quinone reductase（SQR）; directed expression vector; intestinal fatty acid-binding protein（IFABP）; intestinal epithelial cell
分类号:
S634.3
DOI:
10.3969/j.issn.1000-4440.2015.01.015
文献标志码:
A
摘要:
为建立硫化物醌氧化还原酶（SQR）的肠道定向表达载体，同时通过转染小鼠肠道上皮细胞确定载体的有效性，本试验采用重叠PCR、中间载体法、酶切连接法获得重组载体，通过脂质体转染法将重组载体转入小鼠肠道上皮细胞，并鉴定重组载体的特异性表达。结果显示,成功构建了以肠脂肪酸结合蛋白质（IFABP）为启动子的SQR肠道定向表达载体pcDNA3.1(-)-IFABP-SQR-GFP，并在小鼠肠道上皮细胞中检测到表达的融合绿色荧光蛋白质（GFP）。表明IFABP启动子能够在肠道细胞中定向启动SQR的表达。
Abstract:
The directed expression vector pcDNA3.1(-)-IFABP-SQR-GFP for sulfide-quinone reductase（SQR） gene were successfully constructed by means of nested PCR, intermediate vector and restriction enzyme ligation. The vector was transfected into mouse intestinal epithelial cells by lipofection for the expression of SQR gene. The results revealed that the expression of green fluorescent protein was detected in mouse intestinal epithelial cells, indicative of the expression of SQR gene initiated by the promotor of intestinal fatty acid-binding protein (IFABP).

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2014-07-24 基金项目：农业部转基因生物新品种培育重大专项( 2014ZX08006-004)；江苏省农业科技自主创新基金项目[CX(10) 421] 作者简介：于建宁（1980-），女，山东烟台人，博士，副研究员,研究方向为动物生物技术与繁殖内分泌。（Tel）025-84390336；（E-mail）jianningyu @aliyun.com 通讯作者：王公金， （E-mail）wgjphd@163.com
更新日期/Last Update: 2015-02-28