[1]杨雄伟,邓明军,卢昌友,等.施氮量对烟草根际土壤微生物群落及功能的影响[J].江苏农业学报,2026,42(05):952-962.[doi:doi:10.3969/j.issn.1000-4440.2026.05.009]
　YANG Xiongwei,DENG Mingjun,LU Changyou,et al.Effects of nitrogen application rates on microbial community and function in tobacco rhizosphere soil[J].,2026,42(05):952-962.[doi:doi:10.3969/j.issn.1000-4440.2026.05.009]
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施氮量对烟草根际土壤微生物群落及功能的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年05期
页码:
952-962
栏目:
耕作栽培·资源环境
出版日期:
2026-05-31

文章信息/Info

Title:
Effects of nitrogen application rates on microbial community and function in tobacco rhizosphere soil
作者:
杨雄伟1; 邓明军2; 卢昌友3; 黄晓霞1; 卢燕回2; 陈钰栋2; 孔菲2
(1.西南林业大学园林园艺学院,云南昆明650224；2.中国烟草总公司广西壮族自治区公司,广西南宁530022；3.广西壮族自治区烟草公司河池市公司,广西河池547000)
Author(s):
YANG Xiongwei1; DENG Mingjun2; LU Changyou3; HUANG Xiaoxia1; LU Yanhui2; CHEN Yudong2; KONG Fei2
(1.College of Landscape Architecture and Horticulture, Southwest Forestry University, Kunming 650224, China;2.Guangxi Zhuang Autonomous Region Company, China National Tobacco Corporation, Nanning 530022, China;3.Hechi Tobacco Company of Guangxi Zhuang Autonomous Region Tobacco Company, Hechi 547000, China)
关键词:
烟草; 施氮量; 根际土壤; 微生物群落
Keywords:
tobacco; nitrogen application rate; rhizosphere soil; microbial community
分类号:
TS41+4
DOI:
doi:10.3969/j.issn.1000-4440.2026.05.009
文献标志码:
A
摘要:
本研究旨在探究不同施氮量对广西壮族自治区河池烟草种植区烟草根际土壤微生物群落结构和功能的影响,为烤烟生产中氮肥的合理施用提供科学参考。以当地常规施氮量(135 kg/hm2)为对照(CK),同时设置4个施氮量处理，分别是：105 kg/hm2(N1)、120 kg/hm2(N2)、150 kg/hm2(N3)、165 kg/hm2(N4),采用ITS和16S rRNA高通量测序技术,比较烟草植株根际土壤理化性质及微生物群落对不同施氮量的响应。结果表明,随着施氮量增加,土壤pH降低,有机碳(SOC)含量、全氮(TN)含量、全磷(TP)含量、有效磷(AVP)含量、硝态氮(NO-3-N)含量、铵态氮(NH+4-N)含量呈逐渐上升趋势。与CK相比,N2处理下烟草根际土壤优势细菌绿弯菌门细菌、芽单胞菌属细菌、鞘氨醇单胞菌属细菌、小单孢菌属细菌、Candidatus_Solibacter细菌的相对丰度均上升,优势真菌子囊菌门真菌、担子菌门真菌、青霉菌属真菌、暗梗单孢霉属真菌、原隐球菌属真菌、绿核菌属真菌的相对丰度均上升；随施氮量增加,土壤细菌群落多样性指数均呈先增加后降低的趋势,在N2处理时最大,N4处理时最小；土壤真菌群落多样性指数均表现为逐渐降低的趋势,在N1处理时最大,N4处理时最小；真菌功能预测结果表明,与CK相比,N2处理中未分类的腐生真菌、外生菌根真菌、真菌寄生菌-未分类的腐生菌、麦角菌科内生菌-植物病原菌相对丰度增加,植物病原体-土壤腐生菌-木质腐生菌相对丰度降低；细菌功能预测结果表明,类群组成和相对丰度变化不显著；曼特尔（Mantel）检验结果表明,pH、全磷（TP）含量、硝态氮（NO-3-N）含量是影响土壤细菌群落的关键因素,有机碳（SOC）含量是影响土壤真菌群落的关键因素。综上所述,120 kg/hm2施氮量有利于当地烟草根际土壤养分循环、土壤微生物环境的稳定及土壤健康,对于广西烟草种植区的施肥管理有一定的指导意义。
Abstract:
This study aimed to investigate the effects of varying nitrogen application rates on the structure and function of microbial communities in rhizosphere soil of tobacco in Hechi tobacco planting area of Guangxi Zhuang Autonomous Region, providing a scientific reference for rational application of nitrogen fertilizer in flue-cured tobacco production. The local conventional nitrogen application rate (135 kg/hm2) was used as a control (CK). Four nitrogen treatments were established: reduced nitrogen treatments N1 (105 kg/hm2) and N2 (120 kg/hm2), and increased nitrogen treatments N3 (150 kg/hm2) and N4 (165 kg/hm2). High-throughput sequencing techniques, including ITS and 16S rRNA, were employed to compare the responses of the rhizosphere soil physicochemical properties and microbial communities of tobacco to different nitrogen application rates. The results indicated that with the increase of nitrogen application rate, soil pH decreased, while the contents of soil organic carbon (SOC), total nitrogen (TN), total phosphorus (TP), available phosphorus (AVP), nitrate nitrogen (NO-3-N), and ammonium nitrogen (NH+4-N) increased. Compared with CK, the relative abundances of dominant bacteria, such as Chloroflexi, Gemmatimonas, Sphingomonas, Micromonospora, and Candidatus_Solibacter in the N2 treatment increased. The relative abundances of dominant fungi, including Ascomycota, Basidiomycota, Penicillium, Chloridium, Saitozyma, and Ustilaginoidea also increased. Additionally, the diversity index of soil bacterial communities showed a trend of initially increasing and then decreasing with increasing nitrogen application rates, reaching the maximum under N2 treatment and the minimum under N4 treatment. In contrast, the diversity index of soil fungal communities gradually decreased, reaching the maximum under N1 treatment and the minimum under N4 treatment. The results of fungal function prediction showed that compared with CK, in the N2 treatment, the relative abundances of undefined saprotrophic fungi, ectomycorrhizal fungi, fungal parasites-undefined saprotrophs, and Clavicipitaceae endophytes-plant pathogens increased, while the relative abundance of plant pathogens-soil saprotrophs-wood saprotrophs decreased. The results of bacterial function prediction revealed that the taxonomic composition and relative abundance did not change significantly. The Mantel test results indicated that pH, total phosphorus (TP) content, and nitrate nitrogen (NO-3-N) content were key factors affecting the soil bacterial community, while soil organic carbon (SOC) content was a key factor affecting the soil fungal community. In conclusion, a nitrogen application rate of 120 kg/hm2 is beneficial to nutrient cycling, the stability of the soil microbial environment, and soil health in the tobacco rhizosphere, providing useful guidance for fertilization management in the tobacco-growing regions of Guangxi.

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备注/Memo

备注/Memo:
收稿日期：2025-06-25基金项目：中国烟草总公司广西壮族自治区公司科技计划项目(202345000024075)；云南省高层次人才“青年拔尖人才”项目(YNWR-QNBJ-2020-222)作者简介：杨雄伟(1997-),男,云南曲靖人,硕士研究生,主要从事植物生理及土壤生态研究。(E-mail)492348507@qq.com通讯作者：孔菲,(E-mail)75345134@qq.com
更新日期/Last Update: 2026-06-17