[1]高方园,杨冬静,陈晶伟,等.甘薯长喙壳菌效应蛋白全基因组预测与转录组分析[J].江苏农业学报,2026,42(05):919-928.[doi:doi:10.3969/j.issn.1000-4440.2026.05.006]
　GAO Fangyuan,YANG Dongjing,CHEN Jingwei,et al.Genome-wide prediction and transcriptomic analysis for effector proteins in Ceratocystis fimbriata, the causal agent of sweetpotato black rot[J].,2026,42(05):919-928.[doi:doi:10.3969/j.issn.1000-4440.2026.05.006]
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甘薯长喙壳菌效应蛋白全基因组预测与转录组分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年05期
页码:
919-928
栏目:
植物保护
出版日期:
2026-05-31

文章信息/Info

Title:
Genome-wide prediction and transcriptomic analysis for effector proteins in Ceratocystis fimbriata, the causal agent of sweetpotato black rot
作者:
高方园1; 杨冬静1; 陈晶伟1; 2; 吴永旺3; 张成玲1; 马居奎1; 2; 唐伟1; 2; 梁昭1; 佟聪1; 高天奇1; 周小四4; 孙厚俊1; 2
(1.江苏徐淮地区徐州农业科学研究所/农业农村部甘薯生物学与遗传育种重点实验室,江苏徐州221131；2.江苏师范大学生命科学学院,江苏徐州221131；3.苏州帕诺米克生物医药科技有限公司,江苏苏州215125；4.江苏沿海地区农业科学研究所,江苏盐城224002)
Author(s):
GAO Fangyuan1; YANG Dongjing1; CHEN Jingwei1; 2; WU Yongwang3; ZHANG Chengling1; MA Jukui1; 2; TANG Wei1; 2; LIANG Zhao1; TONG Cong1; GAO Tianqi1; ZHOU Xiaosi4; SUN Houjun1; 2
(1.Xuzhou Institute of Agricultural Sciences of the Xuhuai District of Jiangsu Province/Key Laboratory of Biology and Genetic Improvement of Sweetpotato of Ministry of Agriculture and Rural Affairs, Xuzhou 221131, China;2.School of Life Sciences, Jiangsu Normal University, Xuzhou 221131, China;3.Suzhou Panomix Biomedical Technology Co., Ltd., Suzhou 215125, China;4.Institute of Agricultural Sciences in the Coastal District of Jiangsu Province, Yancheng 224002, China)
关键词:
甘薯; 长喙壳菌; 效应子; 转录组; 病原菌-宿主互作; 生物信息学预测
Keywords:
sweetpotato; Ceratocystis fimbriata; effectors; transcriptome; pathogen-host interaction; bioinformatic prediction
分类号:
S531
DOI:
doi:10.3969/j.issn.1000-4440.2026.05.006
文献标志码:
A
摘要:
甘薯黑斑病是由长喙壳菌(Ceratocystis fimbriata)引起的一种严重危害甘薯的真菌性病害。该菌会分泌多种效应子以促进对甘薯的侵染,本研究基于甘薯长喙壳菌全基因组信息,利用生物信息学软件预测及分析其效应子,并结合转录组探讨效应子在甘薯长喙壳菌接种不同甘薯品种中的表达模式及其与宿主的免疫反应关系。结果表明,从甘薯黑斑病病原菌甘薯长喙壳菌全基因组7 266条蛋白质氨基酸序列中共鉴定出292个经典分泌蛋白,经EffectorP 3.0预测得到93个候选效应子,其中PHI数据库可注释到92个效应子的功能。利用eggNOG-mapper分析结果显示,42个效应子具有功能注释,主要涉及信号转导机制,糖类转运和代谢,翻译后蛋白质修饰、蛋白质周转和伴侣蛋白等生理过程。信号肽的特征分析结果表明,信号肽长度集中在16 aa至21 aa,-3位和-1位氨基酸相对保守,其切割位点属于典型的AXA型。转录组数据分析结果显示,接种于徐薯18的甘薯长喙壳菌有57个效应子被激活,接种于南京92的甘薯长喙壳菌有56个效应子被激活,两者共同表达54个效应子。值得注意的是,部分效应子在接种于南京92的甘薯长喙壳菌中表现出显著上调趋势,提示其可能与抗病机制相关。本研究结果为进一步揭示甘薯长喙壳菌的致病机制及其与宿主植物的相互作用提供了重要的分子基础，预测的效应子及转录表达模式为深入研究甘薯免疫反应及其生物防治甘薯黑斑病提供了潜在的靶标。
Abstract:
Sweetpotato black rot, caused by Ceratocystis fimbriata, is a destructive fungal disease that severely impacts sweetpotato production. The pathogen secretes multiple effectors to facilitate its infection of sweetpotato. In the study, we conducted a comprehensive genome-wide prediction and characterization of candidate effectors from Ceratocystis fimbriata, followed by transcriptomic analysis to elucidate their expression patterns in different sweetpotato cultivars and their potential roles in host-pathogen interactions. A total of 292 classical secreted proteins were identified from 7 266 predicted protein sequences in the Ceratocystis fimbriata genome. A total of 93 candidate effectors were predicted by EffectorP 3.0, 92 of which were functionally annotated by the Pathogen-Host Interactions (PHI) database. Functional enrichment using eggNOG-mapper indicated that 42 effectors were associated with critical biological processes, including signal transduction, carbohydrate transport and metabolism, post-translational modifications, protein turnover, and chaperone activity. Signal peptide analysis revealed the length of signal peptide was concentrated between 16 aa to 21 aa, with conserved residues at -3 and -1 positions, and the cleavage site belonged to the typical AXA type. Transcriptome profiling showed that 57 effectors were activated in Ceratocystis fimbriata inoculated into Xushu 18, and 56 effectors were activated in Ceratocystis fimbriata inoculated into Nanjing 92, with 54 effectors co-expressed in both cultivars. Several effectors exhibited a significant upregulation trend in Ceratocystis fimbriata inoculated into Nanjing 92, suggesting that they may be associated with the resistance mechanism. The findings of this study provide an important molecular basis for further elucidating the pathogenic mechanism of Ceratocystis fimbriata and its interaction with host plants. The predicted effectors and their transcriptional expression patterns offer potential targets for in-depth research on sweetpotato immune responses and biological control of sweetpotato black rot.

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备注/Memo

备注/Memo:
收稿日期：2025-06-23基金项目：财政部和农业农村部:国家现代农业产业技术体系项目(CARS-10)；国家自然科学基金项目(32001599)作者简介：高方园(1995-),男,安徽宿州人,硕士,助理研究员,主要从事植物病害研究。(E-mail)fygao030506@163.com通讯作者：周小四,(E-mail)1614971309@qq.com；孙厚俊,(E-mail)sunhoujun1980@163.com
更新日期/Last Update: 2026-06-17