[1]翟心雨,纠敏,魏利辉,等.番茄钙依赖蛋白激酶基因CDPK6负调控植株耐热性[J].江苏农业学报,2026,42(03):606-615.[doi:doi:10.3969/j.issn.1000-4440.2026.03.019]
　ZHAI Xinyu,JIU Min,WEI Lihui,et al.Tomato calcium-dependent protein kinase gene CDPK6 negatively regulates plant thermotolerance[J].,2026,42(03):606-615.[doi:doi:10.3969/j.issn.1000-4440.2026.03.019]
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番茄钙依赖蛋白激酶基因CDPK6负调控植株耐热性()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年03期
页码:
606-615
栏目:
园艺
出版日期:
2026-03-31

文章信息/Info

Title:
Tomato calcium-dependent protein kinase gene CDPK6 negatively regulates plant thermotolerance
作者:
翟心雨1; 2; 纠敏1; 魏利辉2; 周冬梅2
(1.河南科技大学食品与生物工程学院,河南洛阳471023；2.江苏省农业科学院植物保护研究所,江苏南京210014)
Author(s):
ZHAI Xinyu1; 2; JIU Min1; WEI Lihui2; ZHOU Dongmei2
(1.College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China；2.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
番茄; 钙依赖蛋白激酶基因; 基因沉默; 高温胁迫; 耐热性
Keywords:
tomato; calcium-dependent protein kinase gene; gene silencing; high-temperature stress; thermoto-lerance
分类号:
S641.2
DOI:
doi:10.3969/j.issn.1000-4440.2026.03.019
文献标志码:
A
摘要:
为了探究番茄钙依赖蛋白激酶(CDPK)家族在高温胁迫中的功能分化与调控机制,本研究通过定量逆转录聚合酶链反应(qRT-PCR)技术系统解析了番茄CDPK基因在高温胁迫下的表达模式,发现CDPK6基因在高温胁迫1 h及以上相对表达量显著上调。为了验证CDPK6基因对番茄耐热性的影响,利用病毒诱导的基因沉默(VIGS)技术获得CDPK6基因沉默植株,并验证在40 ℃持续高温胁迫下植株表型以及相关生理指标。结果表明,在高温胁迫下，CDPK6基因沉默植株热害指数显著低于野生型(WT)植株；相对电导率显著低于WT植株；超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性随高温胁迫时间增加呈现先下降后持续上升趋势,高温胁迫6 h和12 h时SOD活性显著高于WT植株和绿色荧光蛋白基因（GFP）沉默植株,高温胁迫24 h时POD活性显著高于WT植株和GFP植株,还原型谷胱甘肽（GSH）含量随着高温胁迫时间增加持续上升,高温胁迫24 h时GSH含量显著高于WT植株和GFP植株；光合系统稳定性更强,不仅有效减缓了初始荧光（F0）的升高趋势,还抑制了最大光化学效率(Fv/Fm)的异常提升,OJIP曲线能稳定维持正常生理特征。综上,CDPK6基因沉默植株表现出显著耐热优势,在番茄耐高温胁迫中具有重要应用价值。
Abstract:
To explore the functional differentiation and regulatory mechanisms of the calcium-dependent protein kinase (CDPK) family in tomato under high-temperature stress, this study systematically analyzed the expression patterns of tomato CDPK genes under high-temperature stress using quantitative reverse transcription polymerase chain reaction (qRT-PCR) technology. The results showed that the relative expression level of CDPK6 gene was significantly up-regulated under high-temperature stress for one hour or longer. To verify the effect of the CDPK6 gene on tomato heat tolerance, virus-induced gene silencing (VIGS) technology was used to obtain CDPK6-silenced plants, and the plant phenotype and related physiological indices were determined under continuous high-temperature stress at 40 ℃. The results indicated that, under high-temperature stress, compared with wild-type (WT) plants, the CDPK6-silenced plants had a significantly lower heat injury index and relative electrical conductivity. The activities of superoxide dismutase (SOD) and peroxidase (POD) showed a trend of first decreasing and then continuously increasing with the extension of high-temperature stress duration. At 6 h and 12 h of high-temperature stress, the SOD activity of CDPK6-silenced plants was significantly higher than that of WT plants and green fluorescent protein (GFP)-silenced plants. At 24 h of high-temperature stress, the POD activity was significantly higher than that of WT plants and GFP plants. The content of reduced glutathione (GSH) increased continuously with prolonged high-temperature stress, and was significantly higher in CDPK6-silenced plants than in WT and GFP plants after 24 h of stress. The photosynthetic system of CDPK6-silenced plants exhibited greater stability, which not only effectively slowed down the increasing trend of initial fluorescence (F0) but also inhibited the abnormal increase of maximum photochemical efficiency (Fv/Fm), and the OJIP curves could stably maintain normal physiological characteristics. In conclusion, CDPK6-silenced plants exhibit significant heat tolerance advantages, indicating that the CDPK6 gene has important application value in improving tomato tolerance to high-temperature stress.

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备注/Memo

备注/Memo:
收稿日期：2025-04-25基金项目：江苏省农业科技自主创新基金项目[CX(23)1033]作者简介：翟心雨(2000-),女,内蒙古呼伦贝尔人,硕士研究生,主要从事植物抗逆性研究。(E-mail)xinyuzhai66@163.com通讯作者：纠敏,(E-mail)jiumin0912@163.com;周冬梅,(E-mail)dongmeizhou@jaas.ac.cn
更新日期/Last Update: 2026-04-17