[1]程金花,崔乐康,戴超辉,等.FBXW7基因对脱氧雪腐镰刀菌烯醇(DON)诱导的猪肠上皮细胞铁死亡的调控[J].江苏农业学报,2026,42(01):132-141.[doi:doi:10.3969/j.issn.1000-4440.2026.01.014]
　CHENG Jinhua,CUI Lekang,DAI Chaohui,et al.Regulation of deoxynivalenol-induced ferroptosis in porcine intestinal epithelial cells by FBXW7 gene[J].,2026,42(01):132-141.[doi:doi:10.3969/j.issn.1000-4440.2026.01.014]
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FBXW7基因对脱氧雪腐镰刀菌烯醇(DON)诱导的猪肠上皮细胞铁死亡的调控()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年01期
页码:
132-141
栏目:
畜牧兽医·水产养殖
出版日期:
2026-01-31

文章信息/Info

Title:
Regulation of deoxynivalenol-induced ferroptosis in porcine intestinal epithelial cells by FBXW7 gene
作者:
程金花1; 2; 崔乐康3; 戴超辉1; 2; 李伟宁1; 2; 李辉1; 2; 赵为民1; 2; 李碧侠1; 2; 王学敏1; 2; 廖超1; 2; 包文斌3; 付言峰1; 2
(1.江苏省农业科学院畜牧研究所/农业农村部种养结合重点实验室/江苏省畜禽精准育种工程研究中心,江苏南京210014;2.江苏省农业种质资源保护与利用平台,江苏南京210014;3.扬州大学动物科学与技术学院,江苏扬州225009)
Author(s):
CHENG Jinhua1; 2; CUI Lekang3; DAI Chaohui1; 2; LI Weining1; 2; LI Hui1; 2; ZHAO Weimin1; 2; LI Bixia1; 2; WANG Xuemin1; 2; LIAO Chao1; 2; BAO Wenbin3; FU Yanfeng<
(1.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs/Jiangsu Province Engineering Research Center of Precision Animal Breeding, Nanjing 210014, China;2.Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, China;3.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China)
关键词:
猪; FBXW7基因; 脱氧雪腐镰刀菌烯醇(DON); 肠上皮细胞; 铁死亡
Keywords:
pig; FBXW7 gene; deoxynivalenol (DON); intestinal epithelial cells; ferroptosis
分类号:
S828
DOI:
doi:10.3969/j.issn.1000-4440.2026.01.014
文献标志码:
A
摘要:
为了明确FBXW7基因在脱氧雪腐镰刀菌烯醇(DON)诱导的猪肠上皮细胞铁死亡过程中的调控作用,本研究用DON处理猪肠上皮细胞系(IPEC-J2)细胞,通过实时荧光定量聚合酶链式反应(qPCR)检测细胞中FBXW7基因与铁死亡相关基因的表达水平,通过基因干扰/过表达技术结合qPCR探究FBXW7基因在DON诱导的IPEC-J2细胞损伤过程中的调控作用。结果表明,与未用DON处理的对照(NC)相比,DON处理IPEC-J2细胞后,细胞活力下降、细胞内活性氧(ROS)水平极显著升高(P＜0.01)，细胞中FBXW7基因表达水平显著或极显著上调(P＜0.05或P＜0.01),且铁死亡相关基因表达水平也发生显著或极显著变化(P＜0.05或P＜0.01)。FBXW7基因过表达抑制了铁死亡激活基因的表达,促进了铁死亡抑制基因的表达,最终缓解了DON诱导的IPEC-J2细胞铁死亡。本研究结果初步揭示过表达FBXW7基因可缓解DON诱导的猪肠上皮细胞铁死亡,进而提高猪对DON的抗性,为利用抗病育种策略制订DON预防和控制新方法提供了理论依据。
Abstract:
To elucidate the regulatory role of the FBXW7 gene in deoxynivalenol (DON)-induced ferroptosis in porcine intestinal epithelial cells, IPEC-J2 cells were treated with varying concentrations of DON, and quantitative real-time PCR (qPCR) was used to determine the expression levels of the FBXW7 gene and some ferroptosis-related genes in IPEC-J2 cells under different treatments. Furthermore, gene knockdown and overexpression techniques combined with qPCR were utilized to explore the regulation of FBXW7 in DON-induced IPEC-J2 cell damage. The results demonstrated that DON significantly reduced IPEC-J2 cell viability, and markedly increased intracellular reactive oxygen species (ROS) level (P<0.01). Additionally, DON exposure significantly upregulated FBXW7 gene expression and induced substantial alterations in the expression of ferroptosis pathway-related genes. However, FBXW7 overexpression inhibited the expression of DON-induced ferroptosis-activating genes and promoted the expression of ferroptosis-inhibiting genes, ultimately alleviating DON-induced ferroptosis in IPEC-J2 cells. This study provided preliminary insights into that the overexpression of FBXW7 gene in vitro may be beneficial for alleviating DON induced ferroptosis in pig intestinal epithelial cells, thereby enhancing pig resistance to DON. This study provides a theoretical basis for developing new methods for DON prevention and control using disease resistant breeding strategies.

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备注/Memo

备注/Memo:
收稿日期:2025-08-11基金项目:江苏省种业振兴揭榜挂帅项目[JBGS(2021)099];江苏省青年基金项目(BK20230753);国家生猪产业技术体系项目(CARS-PIG-35);江苏省农业科学院科学仪器开放共享自主研究课题[GX(24)1004]作者简介:程金花(1981-),女,江苏盐城人,博士,副研究员,研究方向为猪遗传育种与繁殖。(E-mail)jhcheng@jaas.ac.cn通讯作者:付言峰,(E-mail)20110009@jaas.ac.cn;包文斌,(E-mail)
更新日期/Last Update: 2026-02-09