[1]王文,朱守晶,胡能兵.辣椒响应高温胁迫的生理与分子机制研究进展[J].江苏农业学报,2025,(12):2462-2471.[doi:doi:10.3969/j.issn.1000-4440.2025.11.018]
 WANG Wen,ZHU Shoujing,HU Nengbing.Research progress on the physiological and molecular mechanisms of pepper in response to high-temperature stress[J].,2025,(12):2462-2471.[doi:doi:10.3969/j.issn.1000-4440.2025.11.018]
点击复制

辣椒响应高温胁迫的生理与分子机制研究进展()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年12期
页码:
2462-2471
栏目:
综述
出版日期:
2025-12-31

文章信息/Info

Title:
Research progress on the physiological and molecular mechanisms of pepper in response to high-temperature stress
作者:
王文12朱守晶12胡能兵12
(1.安徽科技学院农学院,安徽凤阳233100;2.安徽省凤阳县蔬菜种质资源库<安徽科技学院分库>,安徽凤阳233100)
Author(s):
WANG Wen12ZHU Shoujing12HU Nengbing12
(1.School of Agriculture, Anhui Science and Technology University, Fengyang 233100, China;2.Vegetable Germplasm Resource Bank of Fengyang County, Anhui Province (Sub-bank of Anhui Science and Technology University), Fengyang 233100, China)
关键词:
辣椒高温胁迫生理生化响应机制耐热基因多组学研究
Keywords:
Capsicum annuumhigh-temperature stressphysiological and biochemical response mechanismheat-tolerant genesmulti-omics research
分类号:
S641.3
DOI:
doi:10.3969/j.issn.1000-4440.2025.11.018
文献标志码:
A
摘要:
辣椒是中国重要的农业经济作物,市场前景广阔,发展潜力巨大。然而,在设施农业不断发展的背景下,高温胁迫日益成为制约辣椒产业可持续发展的关键因素。解析辣椒响应高温胁迫的生理生化机制,是开展耐热辣椒品种遗传改良的重要基础。本文系统综述了辣椒在高温胁迫下的生理生化响应特征、关键耐热分子机制、多组学研究进展及相关功能基因的挖掘现状,以期为深入探究辣椒耐热机理提供理论依据,并为耐高温辣椒新品种的选育工作提供思路参考,从而推动中国辣椒产业的高质量发展,提升其国际竞争力。
Abstract:
The pepper industry is one of the crucial agricultural sectors in China, boasting broad market prospects and significant development potential. However, with the advancement of protected agriculture, high-temperature stress has become a major constraint on the sustainable development of the pepper industry. Understanding the physiological and biochemical mechanisms underlying the response to heat stress in peppers is essential for the genetic improvement of heat-tolerant varieties. To provide a theoretical basis for in-depth investigation of heat-resistant mechanism in pepper cultivars, this paper reviewd current research on the physiological and biochemical responses to heat stress, key molecular mechanisms advances in multi-omics studies, and the identification of functional genes. The aim is also to provide valuable insights for further research on pepper responses to high-temperature stress, thereby promoting the sustainable development of the pepper industry in China and enhancing its international competitiveness.

参考文献/References:

[1]GAO L L, MA Y Z, WANG P, et al. Transcriptome profiling of Clematis apiifolia:insights into heat-stress responses[J]. DNA and Cell Biology,2017,36(11):938-946.
[2]GUO M, YIN Y X, JI J J, et al. Cloning and expression analysis of heat-shock transcription factor gene CaHsfA2 from pepper (Capsicum annuum L.)[J]. Genetics and Molecular Research,2014,13(1):1865-1875.
[3]赵小刚,隋心意,温祥珍,等. PIF4转录因子调控植物热形态建成研究进展[J]. 植物生理学报,2022,58(3):492-500.
[4]胡能兵,庞丹丹,隋益虎,等. 14种辣椒对高温胁迫的生理响应及抗热性评价[J]. 浙江农业学报,2018,30(7):1168-1174.
[5]金新文,沈征言. 高温胁迫下三种蔬菜抗热性不同的品种间叶片蒸腾强度作用的比较[J]. 石河子大学学报(自然科学版),1997,1(3):25-29.
[6]潘宝贵,王述彬,刘金兵,等. 高温胁迫对不同辣椒品种苗期光合作用的影响[J]. 江苏农业学报,2006,22(2):137-140.
[7]徐剑锋. 甜椒耐热机理及热胁迫下生理、生化变化的研究[D]. 福州:福建农林大学,2003.
[8]刘凯歌,宋云鹏,龚繁荣,等. 高温胁迫对甜椒幼苗生长和生理生化指标的影响[J]. 上海农业学报,2015,31(3):63-67.
[9]贾志银,巩振辉,许红娟,等. 高温胁迫对辣椒幼苗生长及生理性状的影响[J]. 北方园艺,2010(12):5-8.
[10]陈思婷,郭房庆. 植物耐热性及热激信号转导机制研究进展[J]. 中国科学:生命科学,2013,43(12):1072-1081.
[11]吴昊,李燕敏,谢传晓. 作物耐热生理基础与基因发掘研究进展[J]. 作物杂志,2018(5):1-9.
[12]KAN Y, MU X R, GAO J, et al. The molecular basis of heat stress responses in plants[J]. Molecular Plant,2023,16(10):1612-1634.
[13]WAADT R, SELLER C A, HSU P K, et al. Plant hormone regulation of abiotic stress responses[J]. Nature Reviews Molecular Cell Biology,2022,23(10):680-694.
[14]郭文雅,赵京献,郭伟珍. 脱落酸(ABA)生物学作用研究进展[J]. 中国农学通报,2014,30(21):205-210.
[15]张会. 脱落酸在植物抗性生理中的作用[J]. 安徽农业科学,2013,41(2):490-491,527.
[16]刘凯歌,朱月林,郝婷,等. 叶面喷施6-BA对高温胁迫下甜椒幼苗生长和叶片生理生化指标的影响[J]. 西北植物学报,2014,34(12):2508-2514.
[17]OTLORA G, PIERO M C, COLLADO-GONZLEZ J, et al. Exogenous salicylic acid modulates the response to combined salinity-temperature stress in pepper plants (Capsicum annuum L. var. Tamarin)[J]. Plants(Basel),2020,9(12):1790.
[18]李杰. 油菜素内酯调控辣椒低温耐受性的作用机理[D]. 兰州:甘肃农业大学,2016.
[19]KAZAN K. Diverse roles of jasmonates and ethylene in abiotic stress tolerance[J]. Trends in Plant Science,2015,20(4):219-229.
[20]LIAO Z C, GHANIZADEH H, ZHANG X, et al. Exogenous methyl jasmonate mediated MiRNA-mRNA network improves heat tolerance of perennial ryegrass[J]. International Journal of Molecular Sciences,2023,24(13):11085.
[21]宋松泉,刘军,徐恒恒,等. 乙烯的生物合成与信号及其对种子萌发和休眠的调控[J]. 作物学报,2019,45(7):969-981.
[22]余楚英,尹延旭,王飞,等. 茄果类蔬菜热胁迫及耐热性研究进展[J]. 中国蔬菜,2021(4):27-40.
[23]何铁光,董文斌,王爱勤,等. 高温胁迫下辣椒生理生化响应机理初步探讨[J]. 西南农业学报,2013,26(2):541-544.
[24]MA J, LI S, WANG T Y, et al. Cooperative condensation of RNA-DIRECTED DNA METHYLATION 16 splicing isoforms enhances heat tolerance in Arabidopsis[J]. Nature Communications,2025,16(1):433.
[25]LV W T, LIN B, ZHANG M, et al. Proline accumulation is inhibitory to Arabidopsis seedlings during heat stress[J]. Plant Physiology,2011,156(4):1921-1933.
[26]KAVI KISHOR P B, SREENIVASULU N. Is proline accumulation per se correlated with stress tolerance or is proline homeostasis a more critical issue?[J]. Plant Cell and Environment,2014,37(2):300-311.
[27]马宝鹏,逯明辉,巩振辉. 辣椒幼苗对高温胁迫的生长生理响应[J]. 西北农林科技大学学报(自然科学版),2013,41(10):112-118.
[28]RAJAMETOV S N, YANG E Y, CHO M C, et al. Heat-tolerant hot pepper exhibits constant photosynthesis via increased transpiration rate, high proline content and fast recovery in heat stress condition[J]. Scientific Reports,2021,11(1):14328.
[29]蓝茂锋. 水杨酸和Ca2+处理对观赏辣椒耐热性的影响[D]. 福州:福建农林大学,2013.
[30]张宗申,利容千,王建波. 外源Ca2+预处理对高温胁迫下辣椒叶片细胞膜透性和GSH、AsA含量及Ca2+分布的影响[J]. 植物生态学报,2001(2):230-234,262.
[31]GUO M, LIU J H, MA X, et al. The plant heat stress transcription factors (HSFs):structure, regulation, and function in response to abiotic stresses[J]. Frontiers in Plant Science,2016,7:114.
[32]郭猛. 辣椒热胁迫相关基因表达分析及功能研究[D]. 杨凌:西北农林科技大学,2016.
[33]GUO M, LU J P, ZHAI Y F, et al. Genome-wide analysis, expression profile of heat shock factor gene family (CaHsfs) and characterisation of CaHsfA2 in pepper (Capsicum annuum L.)[J]. BMC Plant Biology,2015,15:151.
[34]刘瑞瑶,黄国弘,李海艳,等. 辣椒CaHsfA2上游转录因子的筛选及耐热功能分析[J]. 中国农业科学,2022,55(16):3200-3209.
[35]ALI M, ZHOU Y, GAO C L, et al. Interaction between transcriptional activator BRI1-EMS-SUPPRESSOR 1 and HSPs regulates heat stress tolerance in pepper[J]. Environmental and Experimental Botany,2023,211:105341.
[36]MOU S L, HE W H, JIANG H T, et al. Transcription factor CaHDZ15 promotes pepper basal thermotolerance by activating HEAT SHOCK FACTORA6a[J]. Plant Physiology,2024,195(1):812-831.
[37]UL HAQ S, KHAN A, ALI M, et al. Heat shock proteins:dynamic biomolecules to counter plant biotic and abiotic stresses[J]. International Journal of Molecular Sciences,2019,20(21):5321.
[38]FENG X H, ZHANG H X, ALI M, et al. A small heat shock protein CaHsp25.9 positively regulates heat, salt, and drought stress tolerance in pepper (Capsicum annuum L.)[J]. Plant Physiologyogy and Biochemistry,2019,142:151-162.
[39]黄柳君. 在热和干旱胁迫下辣椒小分子热激蛋白CaHSP16.4的表达特性分析与其功能鉴定[D]. 杨凌:西北农林科技大学,2018.
[40]丁亚东,舒黄英,高崇伦,等. 中国辣椒热激蛋白HSP70基因家族分析[J]. 植物科学学报,2021,39(2):152-162.
[41]叶红,王斌,任飞,等. 园艺植物WRKY基因功能研究进展[J]. 广东农业科学,2023,50(9):68-78.
[42]DANG F F, LIN J H, XUE B P, et al. CaWRKY27 negatively regulates H2O2-mediated thermotolerance in pepper (Capsicum annuum)[J]. Frontiers in Plant Science,2018,9:1633.
[43]YANG S, CAI W W, SHEN L, et al. A CaCDPK29-CaWRKY27b module promotes CaWRKY40-mediated thermotolerance and immunity to Ralstonia solanacearum in pepper[J]. New Phytologist,2022,233(4):1843-1863.
[44]DANG F F, WANG Y N, YU L, et al. CaWRKY40, a WRKY protein of pepper, plays an important role in the regulation of tolerance to heat stress and resistance to Ralstonia solanacearum infection[J]. Plant Cell Environment,2013,36(4):757-774.
[45]LU Q L, HUANG Y, WANG H, et al. CabZIP23 integrates in CabZIP63-CaWRKY40 Cascade and turns CabZIP63 on mounting pepper immunity against Ralstonia solanacearum via physical interaction[J]. International Journal of Molecular Sciences,2022,23(5):2656.
[46]申磊. CabZIP63通过调节CaWRKY40在辣椒抗青枯病和耐高温中起重要作用[D]. 福州:福建农林大学,2016.
[47]CHENG X G, WAN M Y, SONG Y Q, et al. CaSTH2 disables CaWRKY40 from activating pepper thermotolerance and immunity against Ralstonia solanacearum via physical interactionPLoS Genet[J]. Horticulture Research,2024,11(5):uhae066.
[48]张蓓,任福森,赵洋,等. 辣椒响应热胁迫机制的研究进展[J]. 生物技术通报,2023,39(9):37-47.
[49]赵静珂. 辣椒对热胁迫的生理响应及耐热相关基因功能验证[D]. 郑州:河南农业大学,2024.
[50]WANG J, LV J H, LIU Z B, et al. Integration of transcriptomics and metabolomics for pepper (Capsicum annuum L.) in response to heat stress[J]. International Journal of Molecular Sciences,2019,20(20):5042.
[51]LI T, XU X W, LI Y, et al. Comparative transcriptome analysis reveals differential transcription in heat-susceptible and heat-tolerant pepper (Capsicum annum L.) cultivars under heat stress[J]. Journal of Plant Biology,2015,58:411-424.
[52]CHEN Y H, LU Y S, DONG S B, et al. Cloning and expression analysis of ATG8 (Autophagy-Related 8) gene family in solanaceae[J]. Plants,2024,13(20):2924.
[53]王梦园,魏倩睿,李海艳,等. 辣椒MADS-box转录因子基因CaAGL61的耐热功能分析[J]. 中国农业科学,2025,58(8):1604-1616.
[54]UL HAQ S, KHAN A, ALI M, et al. Knockdown of CaHSP60-6 confers enhanced sensitivity to heat stress in pepper (Capsicum annuum L.)[J]. Planta,2019,250(6):2127-2145.
[55]侯晓明. 辣椒NAC转录因子CaNAC064在温度胁迫下功能的探究[D]. 杨凌:西北农林科技大学,2019.
[56]唐雯琛. 辣椒CCCH锌指基因家族的分析及其耐热性功能的初步鉴定[D]. 杭州:浙江大学,2023.
[57]阎本涛,焦可心,赵悦,等. 辣椒囊泡形成相关基因CaSec16耐热功能分析[J]. 园艺学报,2023,50(11):2387-2400.
[58]鲁进萍. 辣椒热响应基因CaHMA-like的表达特性分析及功能初步鉴定[D]. 杨凌:西北农林科技大学,2016.
[59]CAI W W, YANG S, WU R J, et al. CaSWC4 regulates the immunity-thermotolerance tradeoff by recruiting CabZIP63/CaWRKY40 to target genes and activating chromatin in pepper[J]. PLoS Genetics,2022,18(2):e1010023.
[60]贾志银. 辣椒耐热生理生化特性及谷胱甘肽处理效应研究[D]. 杨凌:西北农林科技大学,2010.
[61]高崇伦. 中国辣椒和柔毛辣椒对温度胁迫响应的初步分析[D]. 海口:海南大学,2020.
[62]YANG S, CAI W W, WU R J, et al. Differential CaKAN3-CaHSF8 associations underlie distinct immune and heat responses under high temperature and high humidity conditions[J]. Nature Communications,2023,14(1):4477.
[63]LI H Y, LIANG M M, HUANG G H, et al. Chromatin remodeling factor 28 and heat shock factor A2 activate binding immunoglobulin protein 3 under heat stress[J]. Plant Physiology,2025,197(4):kiaf090.
[64]RAZA A, CHARAGH S, ABBAS S, et al. Assessment of proline function in higher plants under extreme temperatures[J]. Plant Biology,2023,25(3):379-395.
[65]YANG H J, YU G R, LV Z D, et al. Epigenome profiling reveals distinctive regulatory features and cis-regulatory elements in pepper[J]. Genome Biology,2025,26(1):121.
[66]王中一,刘熠,胡博文,等. 基于RUBY及CaREF1的辣椒高效遗传转化体系构建[J]. 园艺学报,2025,52(4):1093-1104.
[67]BULLE M, VENKATAPURAM A K, ABBAGANI S, et al. CRISPR/Cas9 based genome editing of phytoene desaturase (PDS) gene in chilli pepper (Capsicum annuum L.)[J]. Journal of Genetic Engineering and Biotechnology,2024,22(2):100380.
[68]ZHAO C L, LOU H H, LIU Q, et al. Efficient and transformation-free genome editing in pepper enabled by RNA virus-mediated delivery of CRISPR/Cas9[J]. Journal of Integrative Plant Biology,2024,66(10):2079-2082.
[69]KANG B, LEE S, KO D H, et al. Virus-induced systemic and heritable gene editing in pepper (Capsicum annuum L.)[J]. Plant,2025,122(5):e70257.
[70]TANG Y P, SHEN X Y, DENG X, et al. Establishment of an efficient Agrobacterium-mediated transformation system for chilli pepper and its application in genome editing[J]. Plant Biotechnology Journal,2025,23(11):4752-4754.
[71]LING Y, SERRANO N, GAO G, et al. Thermopriming triggers splicing memory in Arabidopsis[J]. Journal of Experimental Botany,2018,69(10):2659-2675.

相似文献/References:

[1]吕敏,苏建坤,白和盛,等.桃蚜取食和机械损伤对番茄和辣椒 PAL、LOX 和 PPO 活性的诱导作用[J].江苏农业学报,2016,(06):1273.[doi:doi:10.3969/j.issn.1000-4440.2016.06.013]
 Lv?Min,SU Jian-kun,BAI He-sheng,et al.The activities of PAL, LOX and PPO in tomato and pepper plants induced by aphid herbivory and mechanical damage[J].,2016,(12):1273.[doi:doi:10.3969/j.issn.1000-4440.2016.06.013]
[2]吴淑华,赵文浩,李廷芳,等.南京辣椒上一种斑驳类型病毒病的分子鉴定[J].江苏农业学报,2015,(06):1284.[doi:doi:10.3969/j.issn.1000-4440.2015.06.014]
 WU Shu-hua,ZHAO Wen-hao,LI Ting-fang,et al.Molecular identification of a virus causing mottle symptoms in pepper leaves in Nanjing[J].,2015,(12):1284.[doi:doi:10.3969/j.issn.1000-4440.2015.06.014]
[3]郭广君,孙茜,刘金兵,等.基于辣椒基因组重测序的InDel标记开发及应用[J].江苏农业学报,2015,(06):1400.[doi:doi:10.3969/j.issn.1000-4440.2015.06.032]
 GUO Guang-jun,SUN Qian,LIU Jin-bing,et al.Development and application of pepper InDel markers based on genome re-sequencing[J].,2015,(12):1400.[doi:doi:10.3969/j.issn.1000-4440.2015.06.032]
[4]张杰,单宝来,田永生,等.葡萄HSP17基因的合成与功能分析[J].江苏农业学报,2017,(03):503.[doi:doi:10.3969/j.issn.1000-4440.2017.03.004]
 ZHANG Jie,SHAN Bao-lai,TIAN Yong-sheng,et al.Synthesis and functional analysis of HSP17 gene in grape[J].,2017,(12):503.[doi:doi:10.3969/j.issn.1000-4440.2017.03.004]
[5]李廷芳,吴淑华,赵文浩,等.青海海东设施辣椒轻斑驳病毒的分子检测[J].江苏农业学报,2017,(04):958.[doi:doi:10.3969/j.issn.1000-4440.2017.04.036]
 LI Ting-fang,WU Shu-hua,ZHAO Wen-hao,et al.Molecular detection of mild mottle virus isolated from pepper in Haidong, Qinghai province[J].,2017,(12):958.[doi:doi:10.3969/j.issn.1000-4440.2017.04.036]
[6]刘潮,韩利红,宋培兵,等.辣椒类甜蛋白基因家族鉴定及表达分析[J].江苏农业学报,2018,(01):122.[doi:doi:10.3969/j.issn.1000-4440.2018.01.018]
 LIU Chao,HAN Li-hong,SONG Pei-bing,et al.Identification and expression analysis of thaumatin-like protein gene in pepper[J].,2018,(12):122.[doi:doi:10.3969/j.issn.1000-4440.2018.01.018]
[7]王运儒,秦玉燕,杨秀娟,等.40%氯虫·噻虫嗪水分散粒剂在辣椒及土壤中的残留消解动态[J].江苏农业学报,2018,(01):207.[doi:doi:10.3969/j.issn.1000-4440.2018.01.030]
 WANG Yun-ru,QIN Yu-yan,YANG Xiu-juan,et al.Dissipation of chlorantraniliprole and thiamethoxam in pepper and soil after field application in the form of 40% water dispersible granules[J].,2018,(12):207.[doi:doi:10.3969/j.issn.1000-4440.2018.01.030]
[8]潘宝贵,钱恒彦,戈伟,等.辣椒应答冷信号转导机制研究进展[J].江苏农业学报,2019,(03):743.[doi:doi:10.3969/j.issn.1000-4440.2019.03.034]
 PAN Bao-gui,QIAN Heng-yan,GE Wei,et al.Research progress of cold signal transduction mechanisms in pepper[J].,2019,(12):743.[doi:doi:10.3969/j.issn.1000-4440.2019.03.034]
[9]梅瑜,王继华,蔡时可,等.金线莲应答高温胁迫的蛋白质组学分析[J].江苏农业学报,2020,(06):1389.[doi:doi:10.3969/j.issn.1000-4440.2020.06.006]
 MEI Yu,WANG Ji-hua,CAI Shi-ke,et al.Proteomics analysis on Anoectochilus roxburghii in response to high temperature stress[J].,2020,(12):1389.[doi:doi:10.3969/j.issn.1000-4440.2020.06.006]
[10]邱爽,张军,何佳琦,等.大豆GmGolS2-1基因高温胁迫诱导表达及转基因烟草鉴定[J].江苏农业学报,2021,(01):38.[doi:doi:10.3969/j.issn.1000-4440.2021.01.005]
 QIU Shuang,ZHANG Jun,HE Jia-qi,et al.Expression of soybean GmGolS2-1 induced by heat stress and identification of GmGolS2-1 transgenic tobacco[J].,2021,(12):38.[doi:doi:10.3969/j.issn.1000-4440.2021.01.005]
[11]张雪莲,罗德旭,杨红,等.外源褪黑素和硒对高温胁迫下辣椒生理特性和抗氧化系统的影响[J].江苏农业学报,2023,(08):1729.[doi:doi:10.3969/j.issn.1000-4440.2023.08.013]
 ZHANG Xue-lian,LUO De-xu,YANG Hong,et al.Effects of exogenous melatonin and selenium on physiological properties and antioxidant systems of chilies under high temperature stress[J].,2023,(12):1729.[doi:doi:10.3969/j.issn.1000-4440.2023.08.013]
[12]李萌楠,乐秀虎,周阳,等.二氢卟吩铁调控辣椒对高温胁迫的耐性及相关基因的表达[J].江苏农业学报,2024,(06):1070.[doi:doi:10.3969/j.issn.1000-4440.2024.06.014]
 LI Mengnan,LE Xiuhu,ZHOU Yang,et al.Regulation of iron chlorine e6 on high temperature stress tolerance and related gene expression in pepper[J].,2024,(12):1070.[doi:doi:10.3969/j.issn.1000-4440.2024.06.014]

备注/Memo

备注/Memo:
收稿日期:2025-02-20基金项目:安徽省高校自然科学研究项目(2023AH051846);安徽省高校理工科教师赴企业挂职实践计划项目(2024jsqygz68);安徽省高校自然科学研究项目(2022AH051626)作者简介:王文(2002-),女,陕西渭南人,硕士研究生,研究方向为辣椒耐热基因功能。(E-mail)wangwen291@sina.com通讯作者:朱守晶,(E-mail)zhusj@ahstu.edu.cn;胡能兵,(E-mail)hunb@ahstu.edu.cn
更新日期/Last Update: 2026-01-20