[1]陆红臣,刘陈玮,王凡,等.基于转录组测序的蚕豆春化响应相关基因挖掘[J].江苏农业学报,2025,(12):2418-2429.[doi:doi:10.3969/j.issn.1000-4440.2025.11.014]
 LU Hongchen,LIU Chenwei,WANG Fan,et al.Mining of genes related to vernalization response in faba bean based on transcriptome sequencing[J].,2025,(12):2418-2429.[doi:doi:10.3969/j.issn.1000-4440.2025.11.014]
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基于转录组测序的蚕豆春化响应相关基因挖掘()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年12期
页码:
2418-2429
栏目:
园艺
出版日期:
2025-12-31

文章信息/Info

Title:
Mining of genes related to vernalization response in faba bean based on transcriptome sequencing
作者:
陆红臣刘陈玮王凡卞晓春徐仁超吴春芳
(江苏沿江地区农业科学研究所,江苏南通226012)
Author(s):
LU HongchenLIU ChenweiWANG FanBIAN XiaochunXU RenchaoWU Chunfang
(Jiangsu Yanjiang Institute of Agricultural Sciences, Nantong 226012, China)
关键词:
蚕豆春化转录组基因挖掘
Keywords:
faba beanvernalizationtranscriptomegene mining
分类号:
S529
DOI:
doi:10.3969/j.issn.1000-4440.2025.11.014
文献标志码:
A
摘要:
为揭示蚕豆春化作用的分子机制,本研究以通鲜2号为材料,通过对春化处理前(CK)、春化处理1 d(V1d)、春化处理10 d(V10d)、春化处理30 d(V30d)的蚕豆茎顶端组织转录组测序,将转录组数据进行两两对比,进行差异表达基因鉴定、功能富集及代谢途径分析,并对8个春化响应基因的表达特征进行PCR验证。结果表明,不同比较组中共鉴定差异表达基因(DEG) 7 553个,DEG主要参与刺激响应、代谢过程、生物调节、结合等生物学功能。春化处理中共筛选、鉴定到34个蚕豆春化响应相关差异基因,涉及春化途径、光周期途径、赤霉素途径等多个途径。实时荧光定量PCR检测结果与转录组测序结果相一致。本研究结果为蚕豆春化响应及花期调控提供了重要的候选基因,同时也为进一步探究蚕豆春化分子机制提供了基础。
Abstract:
In order to reveal the molecular mechanism of vernalization in faba bean, Tongxian 2 was used as the material, transcriptome sequencing was performed on the top of faba bean stem treated with different vernalization time (0 d, 1 d, 10 d, 30 d, which were abbreviated as CK, V1d, V10d and V30d respectively), and the transcriptome data were compared in pairs in this study. Differentially expressed genes were identified, functional enrichment and metabolic pathway analysis were performed, and the expression characteristics of eight vernalization response genes were verified by PCR. The results showed that 7 553 differentially expressed genes (DEGs) were identified in different comparison groups. DEGs were mainly involved in biological functions such as stimulus response, metabolic process, biological regulation and binding. A total of 34 differentially expressed genes related to vernalization response of faba bean were screened and identified in vernalization treatments, involving vernalization pathway, photoperiod pathway, gibberellin pathway and other pathways. The results of real-time fluorescence quantitative PCR were consistent with the results of transcriptome sequencing. The results of this study provide important candidate genes for vernalization response and flowering regulation of faba bean, and also provide a basis for further exploration of the molecular mechanism of vernalization of faba bean.

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备注/Memo

备注/Memo:
收稿日期:2025-01-16基金项目:江苏省种业揭榜挂帅项目[JBGS(2021)058];江苏现代农业产业技术体系建设项目[JATS(2023)158];江苏沿江地区农业科学研究所博士基金项目[YJBS(2023)003]作者简介:陆红臣(1993-),男,河南周口人,博士,助理研究员,主要从事蚕豆春化基因挖掘及育种研究。(E-mail)20230017@jaas.ac.cn通讯作者:吴春芳,(E-mail)jsyias@163.com
更新日期/Last Update: 2026-01-20