[1]李承龙,李琬玉,王耀浦,等.短短芽孢杆菌Rh47代谢物对盐胁迫下黄瓜幼苗离子平衡和营养吸收的影响[J].江苏农业学报,2025,(10):2047-2056.[doi:doi:10.3969/j.issn.1000-4440.2025.10.018]
　LI Chenglong,LI Wanyu,WANG Yaopu,et al.Effects of metabolites produced by Brevibacillus brevis Rh47 on ion balance and nutrient uptake in cucumber seedlings under salt stress[J].,2025,(10):2047-2056.[doi:doi:10.3969/j.issn.1000-4440.2025.10.018]
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短短芽孢杆菌Rh47代谢物对盐胁迫下黄瓜幼苗离子平衡和营养吸收的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年10期
页码:
2047-2056
栏目:
园艺
出版日期:
2025-10-31

文章信息/Info

Title:
Effects of metabolites produced by Brevibacillus brevis Rh47 on ion balance and nutrient uptake in cucumber seedlings under salt stress
作者:
李承龙; 李琬玉; 王耀浦; 赛尔兰·贾纳尔别克; 亚迪佧尔·努尔麦麦提; 郑旭成; 赵芳萍; 翟炳垚; 许红军; 李红
(新疆农业大学园艺学院,新疆乌鲁木齐830000)
Author(s):
LI Chenglong; LI Wanyu; WANG Yaopu; Saierlan·Jianaerbieke; Yadikaer·Nuermaimaiti; ZHENG Xucheng; ZHAO Fangping; ZHAI Bingyao; XU Hongjun; LI Hong
(College of Horticulture, Xinjiang Agricultural University, Urumqi 830000, China)
关键词:
短短芽孢杆菌Rh47代谢物; 黄瓜; 盐胁迫; 离子平衡; 营养吸收
Keywords:
Brevibacillus brevis Rh47 metabolites; cucumber; salt stress; ionic homeostasis; nutrient uptake
分类号:
S642.2
DOI:
doi:10.3969/j.issn.1000-4440.2025.10.018
文献标志码:
A
摘要:
盐胁迫是影响黄瓜生产的主要非生物逆境因子,大量研究结果表明一些根际促生菌可以提高植物耐盐性,然而这些促生菌的分泌物能否缓解植物的盐胁迫还有待进一步研究。本研究以黄瓜津优401和短短芽孢杆菌Rh47为材料,将黄瓜种子分别浸泡在含有0（对照)、25%、50%、75%、100%的Rh47代谢物中,处理后分别以0 mmol/L、25 mmol/L、50 mmol/L、75 mmol/L NaCl溶液进行盐处理,研究不同含量代谢物对盐胁迫下黄瓜种子发芽的影响。并在75 mmol/L的NaCl胁迫下,用不同含量的Rh47代谢物浇灌黄瓜幼苗,研究其对盐胁迫下黄瓜幼苗生长、植株光合参数以及植株体内K+、Na+、Ca2+等含量和对营养元素吸收的影响。结果表明,在75 mmol/L NaCl处理下,短短芽孢杆菌Rh47代谢物含量为50%时黄瓜种子的活力指数和总根长显著高于其他含量处理(P＜0.05)。在幼苗期,当短短芽孢杆菌Rh47代谢物含量为75%时,与对照相比,盐胁迫下黄瓜幼苗的株高、叶面积、总根长、根表面积和根体积均显著增加(P＜0.05)，幼苗净光合速率、气孔导度和蒸腾速率与对照相比显著提高了49%、59%和44%,地上部K+含量显著增加,全株Na+含量显著降低(P＜0.05)。此外,含量为75%的短短芽孢杆菌Rh47代谢物还显著促进了黄瓜幼苗对Zn、Fe、S和P等营养元素的吸收。综上,适宜含量的短短芽孢杆菌Rh47的代谢物可通过调节黄瓜植株体内离子平衡、增强植株对营养元素的吸收以及提升叶片的光合作用效率,来提高黄瓜耐盐性。
Abstract:
Salt stress is a major abiotic stress factor affecting cucumber production. Numerous studies have shown that certain plant growth-promoting rhizobacteria (PGPR) can enhance plant salt tolerance, but whether their metabolites can alleviate salt stress in plants requires further investigation. This study used cucumber ‘Jinyou 401’ and Brevibacillus brevis Rh47 as test materials. Seeds were soaked in solutions containing 0 (control), 25%, 50%, 75%, and 100% Rh47 metabolites, followed by salt treatments with 0 mmol/L, 25 mmol/L, 50 mmol/L, and 75 mmol/L NaCl solutions to investigate the effects of different metabolite concentrations on cucumber seed germination under salt stress. Under 75 mmol/L NaCl stress, cucumber seedlings were irrigated with different concentrations of Rh47 metabolites to examine their effects on seedling growth, photosynthetic parameters, the contents of K+, Na+, Ca2+ and absorption of nutrient elements. Results demonstrated that under 75 mmol/L NaCl treatment， the vigor index and total root length of cucumber seeds treated with 50% concentration of Brevibacillus brevis Rh47 metabolites were significantly higher than those in other concentration treatments (P<0.05). At the seedling stage, when the metabolite concentration was 75%, cucumber seedlings subjected to salt stress exhibited significant increases (P<0.05) in plant height, leaf area, root length, root surface area, and root volume compared to the control group. Leaf net photosynthetic rate, stomatal conductance, and transpiration rate increased by 49%, 59%, and 44% respectively versus control. Shoot K+ content significantly increased (P<0.05), while whole-plant Na+ content significantly decreased (P<0.05). Additionally, the 75% concentration of Brevibacillus brevis Rh47 metabolites significantly promoted the absorption of nutrient elements such as Zn, Fe, S, and P in cucumber seedlings. In conclusion, Brevibacillus brevis Rh47 metabolites at appropriate concentrations can improve the salt tolerance of cucumber by regulating ionic homeostasis, improving nutrient uptake, and enhancing photosynthetic efficiency.

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备注/Memo

备注/Memo:
收稿日期：2025-03-08基金项目：国家自然科学基金项目(32360790)；新疆维吾尔自治区重点研发项目(2022B02023-2)；新疆蔬菜产业技术体系项目(XJARS-07)；新疆维吾尔自治区2021年“天池博士计划”项目作者简介：李承龙(1999-),男,山东济南人,硕士研究生,主要从事园艺植物与微生物互作方向研究。(E-mail)Chenglong0814@163.com通讯作者：李红,(E-mail)lihongcau@126.com
更新日期/Last Update: 2025-11-17