[1]刘琴,张琥,夏杨,等.核型多角体病毒SfMNPY-Yz01对草地贪夜蛾解毒酶与防御酶活性的影响[J].江苏农业学报,2025,(10):1920-1925.[doi:doi:10.3969/j.issn.1000-4440.2025.10.005]
　LIU Qin,ZHANG Hu,XIA Yang,et al.Effects of nucleopolyhedrovirus SfMNPY-Yz01 on the activities of detoxifying enzymes and defensive enzymes in Spodoptera frugiperda[J].,2025,(10):1920-1925.[doi:doi:10.3969/j.issn.1000-4440.2025.10.005]
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核型多角体病毒SfMNPY-Yz01对草地贪夜蛾解毒酶与防御酶活性的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年10期
页码:
1920-1925
栏目:
植物保护
出版日期:
2025-10-31

文章信息/Info

Title:
Effects of nucleopolyhedrovirus SfMNPY-Yz01 on the activities of detoxifying enzymes and defensive enzymes in Spodoptera frugiperda
作者:
刘琴1; 张琥2; 夏杨1; 林曼曼1; 韩光杰1; 李传明1; 张楠1; 陆玉荣1; 黄立鑫1; 徐健1; 2
(1.江苏里下河地区农业科学研究所/国家农业微生物扬州观测实验站,江苏扬州225007；2.扬州大学植物保护学院,江苏扬州225009)
Author(s):
LIU Qin1; ZHANG Hu2; XIA Yang1; LIN Manman1; HAN Guangjie1; LI Chuanming1; ZHANG Nan1; LU Yurong1; HUANG Lixin1; XU Jian1; 2
(1.Institute of Agricultural Sciences of the Lixiahe District in Jiangsu Province/National Experimental Station of Yangzhou for Agricultural Microbiology, Yangzhou 225007, China；2.College of Plant Protection, Yangzhou University, Yangzhou 225009, China)
关键词:
草地贪夜蛾; 核型多角体病毒; 解毒酶; 防御酶
Keywords:
Spodoptera frugiperda; nucleopolyhedrovirus; detoxifying enzymes; defensive enzymes
分类号:
S433.4
DOI:
doi:10.3969/j.issn.1000-4440.2025.10.005
文献标志码:
A
摘要:
本研究以草地贪夜蛾核型多角体病毒SfMNPV-Yz1毒株感染草地贪夜蛾幼虫,系统测定了其体内酚氧化酶(PO)、谷胱甘肽转移酶(GST)、羧酸酯酶(CarE)、过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)等6种关键解毒酶与防御酶活性在感染过程中的动态变化。研究结果表明,在感染期间,酚氧化酶(PO)活性波动剧烈,可能与病毒调控昆虫黑化反应有关。谷胱甘肽转移酶(GST)持续保持高活性,推测其在缓解氧化应激及代谢有毒物质中发挥作用。在感染后期,过氧化氢酶(CAT)、过氧化物酶(POD)活性显著升高,表明其参与清除过量活性氧(ROS),以维持细胞稳态。本研究从酶活性层面阐明了杆状病毒侵染引发的草地贪夜蛾幼虫多层次生理响应,为草地贪夜蛾生物防治提供了理论依据。
Abstract:
In this study, larvae of Spodoptera frugiperda were infected with the SfMNPV-Yz1 strain of Spodoptera frugiperda nucleopolyhedrovirus. The dynamic changes in the activities of six key detoxifying and defensive enzymes in the larvae during the infection process were systematically determined, including phenol oxidase (PO), glutathione S-transferase (GST), carboxylesterase (CarE), catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD). The results showed that during the infection period, the activity of PO fluctuated drastically, which might be associated with the virus regulating the melanization response in insects. Glutathione S-transferase (GST) maintained high activity consistently, suggesting its role in alleviating oxidative stress and metabolizing toxic substances. In the late stage of infection, the activities of catalase (CAT) and peroxidase (POD) increased significantly, indicating their involvement in scavenging excessive reactive oxygen species (ROS) to maintain cellular homeostasis. This study clarifies the multi-level physiological responses of Spodoptera frugiperda larvae to baculovirus infection from the perspective of enzyme activity, providing a theoretical basis for the biological control of Spodoptera frugiperda.

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备注/Memo

备注/Memo:
收稿日期：2025-03-31基金项目：江苏省农业科技自主创新基金项目(CX-23-3004)；农业基础性长期性科技工作项目(NAES069AM04、NAES-EE-041)；扬州市生态农业重点实验室项目(YZ2023244)作者简介：刘琴(1966-),女,江苏海门人,硕士,研究员,主要从事害虫生物防治技术研究。(E-mail)bio-lq@126.com通讯作者：李传明,(E-mail)liming0595@163.com；徐健,(E-mail)bio-xj@163.com
更新日期/Last Update: 2025-11-17