[1]杨芩,付燕,刘雅兰,等.高糖枇杷果实发育后期淀粉和蔗糖代谢的转录组分析[J].江苏农业学报,2025,(09):1833-1843.[doi:doi:10.3969/j.issn.1000-4440.2025.09.018]
　YANG Qin,FU Yan,LIU Yalan,et al.Transcriptome analysis of starch and sucrose metabolism during the late development stage of high-sugar loquat fruits[J].,2025,(09):1833-1843.[doi:doi:10.3969/j.issn.1000-4440.2025.09.018]
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高糖枇杷果实发育后期淀粉和蔗糖代谢的转录组分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年09期
页码:
1833-1843
栏目:
园艺
出版日期:
2025-09-30

文章信息/Info

Title:
Transcriptome analysis of starch and sucrose metabolism during the late development stage of high-sugar loquat fruits
作者:
杨芩1; 2; 付燕3; 刘雅兰1; 2; 张婷渟1; 2; 彭舒1; 2; 邓洁1; 2
(1.凯里学院省级名师杨芩工作室,贵州凯里556000；2.凯里学院大健康学院,贵州凯里556000；3.黔东南民族职业技术学院,贵州凯里556000)
Author(s):
YANG Qin1; 2; FU Yan3; LIU Yalan1; 2; ZHANG Tingting1; 2; PENG Shu1; 2; DENG Jie1; 2
(1.Provincial Famous Teacher Yang Qin Studio, Kaili University, Kaili 556000, China；2.College of Life and Health Science, Kaili University, Kaili 556000, China；3.Qiandongnan Nationalities Polytechnic, Kaili 556000, China)
关键词:
枇杷; 糖积累; 转录组; 差异表达基因
Keywords:
cumulation; transcriptome; differentially expressed genes
分类号:
S667.3
DOI:
doi:10.3969/j.issn.1000-4440.2025.09.018
文献标志码:
A
摘要:
本研究对开花后第150 d(Ej-FS1)、160 d(Ej-FS2)、170 d(Ej-FS3)、180 d(Ej-FS4)和190 d(Ej-FS5)5个阶段的枇杷果实进行了系统的转录组分析。从Ej-FS1和Ej-FS2比较组、Ej-FS2和Ej-FS3比较组、Ej-FS3和Ej-FS4比较组、Ej-FS4和Ej-FS5比较组中共筛选到17 564个差异表达基因。Ej-FS3和Ej-FS4比较组共有8 584个差异表达基因,其中富集到淀粉和蔗糖代谢通路的差异表达基因有243个,这些差异表达基因调控淀粉和蔗糖代谢通路中的胞质中性转化酶、蔗糖-6-磷酸酶、蔗糖磷酸合成酶、蔗糖合成酶、果糖激酶、葡萄糖-6-磷酸异构酶等25种酶。通过qRT-PCR检测到的差异表达基因的表达模式和转录组数据一致,表明本研究通过高通量测序获得的转录组数据具有可靠性。本研究结果揭示了枇杷果实糖积累的分子机制,为枇杷分子育种提供了重要的候选基因和理论依据。
Abstract:
In this study, a systematic transcriptome analysis was conducted on loquat fruits at five stages, namely the 150th day after flowering (Ej-FS1), the 160th day (Ej-FS2), the 170th day (Ej-FS3), the 180th day (Ej-FS4), and the 190th day (Ej-FS5). A total of 17 564 differentially expressed genes were screened from the comparison groups of Ej-FS1 and Ej-FS2, Ej-FS2 and Ej-FS3, Ej-FS3 and Ej-FS4, and Ej-FS4 and Ej-FS5. There were 8 584 differentially expressed genes in the comparison group of Ej-FS3 and Ej-FS4, among which 243 differentially expressed genes were enriched in the starch and sucrose metabolism pathway. These differentially expressed genes regulated 25 enzymes such as cytosolic neutral invertase, sucrose-6-phosphatase, sucrose phosphate synthase, sucrose synthase, fructokinase, and glucose-6-phosphate isomerase in the starch and sucrose metabolism pathway. The expression patterns of the differentially expressed genes detected by qRT-PCR were consistent with the transcriptome data, indicating that the transcriptome data obtained through high-throughput sequencing in this study are reliable. The results of this study reveal the molecular mechanism of sugar accumulation in loquat fruits and provide important candidate genes and a theoretical basis for molecular breeding of loquat.Key words：loquat；sugar ac

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备注/Memo

备注/Memo:
收稿日期：2024-11-23基金项目：凯里学院省级名师杨芩工作室项目(MSGZS-SJ-2024002)；黔东南州科技计划项目[黔东南科合基础(2022)06号]；贵州省高等学校贵州山地特色园艺作物分子育种与品种创制重点实验室项目[黔教技(2022)053号]；凯里学院一流学科(园艺学)作者简介：杨芩(1983-),男,贵州施秉人,博士,教授,研究方向为果树种质创新与营养品质调控。(E-mail)yangqin1028518@126.com
更新日期/Last Update: 2025-10-27