[1]陈慧杰,毛俊丹,邓喆,等.绣球花叶枯病病原菌鉴定、生物学特性及杀菌剂室内毒力测定[J].江苏农业学报,2025,(08):1499-1507.[doi:doi:10.3969/j.issn.1000-4440.2025.08.006]
　CHEN Huijie,MAO Jundan,DENG Zhe,et al.Identification and biological characterization of the pathogen causing leaf blight on Hydrangea macrophylla and in vitro toxicity bioassay of fungicides[J].,2025,(08):1499-1507.[doi:doi:10.3969/j.issn.1000-4440.2025.08.006]
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绣球花叶枯病病原菌鉴定、生物学特性及杀菌剂室内毒力测定()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年08期
页码:
1499-1507
栏目:
植物保护
出版日期:
2025-08-31

文章信息/Info

Title:
Identification and biological characterization of the pathogen causing leaf blight on Hydrangea macrophylla and in vitro toxicity bioassay of fungicides
作者:
陈慧杰1; 毛俊丹1; 2; 邓喆3; 刘欣童1; 陈双双1; 冯景1; 金玉妍1; 邓衍明1; 2
(1.江苏省农业科学院休闲农业研究所,江苏南京210014；2.安徽工业大学建筑工程学院,安徽马鞍山243032；3.南京工业大学生物与制药工程学院,江苏南京211816)
Author(s):
CHEN Huijie1; MAO Jundan1; 2; DENG Zhe3; LIU Xintong1; CHEN Shuangshuang1; FENG Jing1; JIN Yuyan1; DENG Yanming1; 2
(1.Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.School of Civil Engineering and Architecture, Anhui University of Technology, Ma’anshan 243032, China;3.College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China)
关键词:
绣球花; 叶枯病; 病原菌鉴定; 生物学特性; 毒力测定
Keywords:
Hydrangea macrophylla; leaf blight; pathogen identification; biological characteristics; toxicity test
分类号:
S432.44
DOI:
doi:10.3969/j.issn.1000-4440.2025.08.006
文献标志码:
A
摘要:
为了明确绣球花叶枯病的病原菌,本研究通过组织分离法对采集自江苏省南京市的绣球品种无尽夏叶片发病组织进行分离,经回接试验确定菌株YK-B是致病菌,研究发现,YK-B菌株接种至健康绣球花叶片上能引起和采集的绣球花叶片发病组织一致的叶枯病症状。通过形态学观察并结合多基因联合系统发育分析,病原菌菌株YK-B被鉴定为链格孢菌(Alternaria alternata)；进一步进行病原菌菌株YK-B在不同碳源、氮源、温度和pH值条件下的菌丝生长试验,结果表明,YK-B菌丝生长最适碳源是棉子糖,最适氮源是蛋白胨,最适温度是25 ℃,适宜的pH值为6～8。通过测定7种杀菌剂对致病菌菌株YK-B的室内毒力,结果表明杀菌剂氢氧化铜的抑菌效果最好。本研究结果对绣球花叶枯病的诊断和防治具有一定的指导意义。
Abstract:
To identify the pathogen responsible for hydrangea leaf blight, a tissue isolation method was employed on diseased leaf samples of Hydrangea macrophylla Endless Summer collected from Nanjing City, Jiangsu Province, China. Through back-inoculation experiments, strain YK-B was identified as the pathogenic fungus. The study found that inoculation of healthy hydrangea leaf tissues with strain YK-B induced leaf blight symptoms consistent with those observed on the originally collected diseased samples. Based on morphological characteristics and multigene phylogenetic analysis, the pathogenic strain YK-B was identified as Alternaria alternata. Furthermore, mycelial growth experiments were conducted with the pathogenic strain YK-B under varying conditions of carbon sources, nitrogen sources, temperature, and pH. The results indicated that the optimal conditions for mycelial growth of YK-B were raffinose as the carbon source, peptone as the nitrogen source, a temperature of 25 ℃, and a pH range of 6 to 8. The results of in vitro toxicity assays of seven fungicides against the pathogenic strain YK-B revealed that copper hydroxide exhibited the most effective inhibition. The findings of this study provide practical guidance for the diagnosis and management of hydrangea leaf blight.

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备注/Memo

备注/Memo:
收稿日期：2024-10-21基金项目：江苏省农业科技自主创新基金项目[CX(2023)3062];华东地区花卉生物学国家林业和草原局重点实验室开放基金项目(KFE202401)；中央财政林业科技推广示范项目[苏(2024)TG03)]作者简介：陈慧杰(1989-),女,河南周口人,博士,助理研究员,从事观赏植物栽培与植物病理学研究。(E-mail)chenhuijie2020@163.com通讯作者：邓衍明,(E-mail)nksdym@163.com
更新日期/Last Update: 2025-09-23