[1]孙忠戈,陆莹,刘东旭,等.天山郁金香种子解除休眠过程中生理生化的变化[J].江苏农业学报,2025,(05):977-983.[doi:doi:10.3969/j.issn.1000-4440.2025.05.016]
　SUN Zhongge,LU Ying,LIU Dongxu,et al.Physiological and biochemical changes during the dormancy release process of Tulipa thianschanica Regel seeds[J].,2025,(05):977-983.[doi:doi:10.3969/j.issn.1000-4440.2025.05.016]
点击复制

天山郁金香种子解除休眠过程中生理生化的变化()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年05期
页码:
977-983
栏目:
园艺
出版日期:
2025-05-31

文章信息/Info

Title:
Physiological and biochemical changes during the dormancy release process of Tulipa thianschanica Regel seeds
作者:
孙忠戈1; 2; 陆莹1; 2; 刘东旭1; 雷家军2; 赵珺2; 张伟2
(1.辽宁经济职业技术学院,辽宁沈阳110122;2.沈阳农业大学园艺学院,辽宁沈阳110866)
Author(s):
SUN Zhongge1; 2; LU Ying1; 2; LIU Dongxu1; LEI Jiajun2; ZHAO Jun2; ZHANG Wei2
(1.Liaoning Vocational and Technical College of Economics, Shenyang 110122, China;2.College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China)
关键词:
天山郁金香; 种子; 休眠; 酶活性; 激素
Keywords:
Tulipa thianschanica Regel; seed; dormancy; enzyme activity; hormone
分类号:
S682.263
DOI:
doi:10.3969/j.issn.1000-4440.2025.05.016
文献标志码:
A
摘要:
本研究以4 ℃层积处理天山郁金香种子,系统分析了种子休眠解除过程中生理生化的变化。研究结果表明,在贮藏物质含量方面,随着层积时间的延长,种子可溶性糖含量呈先降低后升高的变化趋势,淀粉含量呈现先升高后降低的趋势,可溶性蛋白含量持续下降。在酶活性方面,随着层积时间的延长,种子过氧化物酶(POD)活性呈先降低后升高的变化趋势。在层积前期（1～10 d）,天山郁金香种子葡萄糖-6-磷酸脱氢酶(G-6-PDH)+6-磷酸葡萄糖酸脱氢酶(6-PGDH)的活性无明显变化;在层积后期(40 d),种子葡萄糖-6-磷酸脱氢酶(G-6-PDH)+6-磷酸葡萄糖酸脱氢酶(6-PGDH)的活性显著升高。内源激素含量方面,随着层积时间的延长,种子脱落酸(ABA)含量呈先升高后降低的变化趋势,赤霉素(GA3)含量呈先降低后升高的变化趋势,生长素(IAA)和细胞分裂素(CTK)含量持续上升。本研究结果揭示了天山郁金香种子解除休眠过程中贮藏物质含量、酶活性以及激素含量的变化规律,为天山郁金香种子的休眠调控和高效繁育提供了理论依据。
Abstract:
In this study, seeds of Tulipa thianschanica Regel were subjected to stratification at 4 ℃ to systematically analyze the physiological and biochemical changes during the process of seed dormancy release. The results showed that in terms of seed storage substances, with the extension of stratification time, the content of soluble sugars in the seeds first decreased and then increased, the content of starch first increased and then decreased, and the content of soluble proteins continuously declined. In terms of seed enzyme activity, with the extension of stratification time, the activity of peroxidase (POD) in the seeds first decreased and then increased. In the early stratification stage (1-10 days), the activities of glucose-6-phosphate dehydrogenase (G-6-PDH) and 6-phosphogluconate dehydrogenase (6-PGDH) in the seeds showed no significant change. However, in the late stratification stage (40 days), the activities of both enzymes in the seeds significantly increased. In terms of endogenous hormone content, with the extension of stratification time, the content of abscisic acid (ABA) in the seeds first increased and then decreased, the content of gibberellin (GA3) first decreased and then increased, while the contents of auxin (IAA) and cytokinin (CTK) continuously rose. The results of this study elucidated the changes in the content of storage substances, enzyme activities, and hormone levels during the dormancy release of Tulipa thianschanica Regel seeds, providing a theoretical basis for the dormancy regulation and efficient propagation of Tulipa thianschanica Regel seeds.

参考文献/References:

[1]产祝龙,向林,王艳平. 郁金香种质资源、育种进展及种球国产化思考[J]. 华中农业大学学报(自然科学版),2022,41(2):144-150.
[2]XING G M, QU L W, ZHANG Y Q, et al. Collection and evaluation of wild tulip (Tulipa spp.) resources in China[J]. Genetic Resources and Crop Evolution,2017,64:641-652.
[3]ZHANG W, ZHAO J, XUE L, et al. Seed morphology and germination of native Tulipa species[J]. Agriculture-Basel,2023,13(2):466.
[4]孙征光,燕阳,刘瑶,等. 天山郁金香种子内源萌发抑制物活性研究[J]. 种子,2024,43(9):23-30,39.
[5]ANGELOVICI R, GALILI G, FERNIE A R, et al. Seed desiccation:a bridge between maturation and germination[J]. Trends in Plant Science,2010,15(4):211-218.
[6]闫青青. 燕山花椒种子休眠及萌发特性研究[D]. 秦皇岛:河北科技师范学院,2022.
[7]李戈,唐玲,王艳芳,等. 不同层积条件下滇重楼种子的生理变化[J]. 中国农学通报,2015,31(7):149-153.
[8]孙海燕,李强,朱铭玮,等. 油用牡丹‘凤丹’种子层积过程中营养物质的代谢变化研究[J]. 南京林业大学学报(自然科学版),2021,45(1):70-78.
[9]孙晓梅,王思思,杨宏光,等. 芍药种子萌发过程中抗氧化酶活性变化规律的研究[J]. 北方园艺,2015(14):53-55.
[10]SHU K, LIU X, XIE Q, et al. Two faces of one seed:hormonal regulation of dormancy and germination[J]. Molecular Plant,2016,9:34-45.
[11]TUAN P A, KUMAR R, REHAL P K, et al. Molecular mechanisms underlying abscisic acid/gibberellin balance in the control of seed dormancy and germination in cereals[J]. Frontiers in Plant Science,2018,9:668.
[12]AGHILIAN B, MOHAMMAD K H, SEPIDEH A. Evaluation of seed dormancy in forty medicinal plant species[J]. International Journal of Agriculture and Crop Sciences,2014,7:760-768.
[13]DAVIERE J M, ACHARD P. Gibberellin signaling in plants[J]. Development,2013,140(6):1147-1151.
[14]BAI B, PEVIANI A, VAN HORST S, et al. Extensive translational regulation during seed germination revealed by polysomal profiling[J]. New Phytologist,2017,214(1):233-244.
[15]金飚,丁玲,何小弟,等. 芍药种子露地播种和低温贮藏过程中内源激素含量的变化[J]. 南京林业大学学报(自然科学版),2006,30(1):45-48.
[16]柳苗,高捍东,高燕,等. 休眠解除过程中紫楠种子生理生化特征的变化[J]. 南京林业大学学报(自然科学版),2023,47(2):9-17.
[17]刘海英,王华华,崔长海,等. 可溶性糖含量测定(蒽酮法)实验的改进[J]. 实验室科学,2013,16(2):19-20.
[18]杨静华. 考马斯亮蓝法测定苦荞麦中可溶性蛋白的含量[J]. 山西医药杂志,2018,47(2):206-207.
[19]李合生. 现代植物生理学[M]. 3版. 北京:高等教育出版社,2012.
[20]王蕾,黄俊华,王佳琪,等. 大白花贝母形态休眠期种子内含物变化对种胚发育的影响[J]. 分子植物育种,2021,19(5):1674-1680.
[21]赖晓辉,李群. 种子休眠与萌发分子机制研究进展[J]. 种子,2014,33(5):53-58.
[22]CARRUGGIO F, ONOFRI A, IMPELLUSO C, et al. Seed dormancy breaking and germination in Bituminaria basaltica and B. bituminosa (Fabaceae) [J]. Plants-Basel,2020,9:1110.
[23]KUREK K, PLITTA B, RATAJCZAK E. Reactive oxygen species as potential drivers of the seed aging process[J]. Plants-Basel,2019,8:174.
[24]GERARDO C, JULIAN C, MONICA P, et al. An updated overview on the regulation of seed germination[J]. Plants-Basel,2020,9(6):703.
[25]于兰莉,王瑶瑶. 白心火龙果与红心火龙果蛋白质含量比较[J]. 现代食品,2020,26(16):198-200.
[26]BAILLY C. The signalling role of ROS in the regulation of seed germination and dormancy[J]. Biochemical Journal,2019,476:3019-3032.
[27]BUIJS G, KODDE J, GROOT S P C, et al. Seed dormancy release accelerated by elevated partial pressure of oxygen is associated with DOG loci[J]. Journal of Experimental Botany,2018,69(15):3601-3608.
[28]闫芳. 黄瑞香种子休眠机理研究[D]. 兰州：甘肃农业大学,2017.
[29]KUCERA B, COHN M A, LEUBNER G. Plant hormone interactions during seed dormancy release and germination[J]. Seed Science Research,2005,15(4):281-307.
[30]YANG L E, PENG D L, LI Z M, et al. Cold stratification, temperature, light, GA3, and KNO3 effects on seed germination of Primula beesiana from Yunnan, China[J]. Plant Diversity,2020,42(3):168-173.
[31]SCHMITZ N, ABRAMS S R, KERMODE A R. Changes in ABA turn over and sensitivity that accompany dormancy termination of yellow cedar (Chamaecyparis nootkatensis) seeds[J]. Journal of Experimental Botany,2002,53(366):89-101．
[32]王海波,高东升,王孝娣,等. 赤霉素和脱落酸与桃芽自然休眠诱导[J]. 果树学报,2006,23(4):599-601.
[33]段成国,李宪利,高东升,等. 内源ABA和GA3对欧洲甜樱桃花芽自然休眠的调控[J]. 园艺学报,2004,31(2):149-154.
[34]JACOBSEN J V, PEARCE D W, POOLE A T, et al. Abscisic acid, phaseic acid and gibberellin contents associated with dormancy and germination in barley[J]. Physiologia Plantarum,2002,115(3):428-441.

相似文献/References:

[1]初龙,李伟,李欣亚,等.重金属超富集植物种子内生真菌多样性及其重金属抗性[J].江苏农业学报,2017,(01):43.[doi:10.3969/j.issn.1000-4440.2017.01.007 ]
　CHU Long,LI Wei,LI Xin ya,et al.Diversity and heavy metal resistance of endophytic fungi from seeds of hyperaccumulators[J].,2017,(05):43.[doi:10.3969/j.issn.1000-4440.2017.01.007 ]
[2]徐慧敏,郭磊,马瑞娟,等.低温冷藏对桃砧木Nemaguard种子萌发及幼苗生长的影响[J].江苏农业学报,2022,38(01):200.[doi:doi:10.3969/j.issn.1000-4440.2022.01.024]
　XU Hui-min,GUO Lei,MA Rui-juan,et al.Effects of cold storage on seed germination and seedling growth of peach rootstock cultivar Nemaguard[J].,2022,38(05):200.[doi:doi:10.3969/j.issn.1000-4440.2022.01.024]
[3]徐德聪,王宇,沈章军,等.不同生境剑叶金鸡菊瘦果和种子的特性[J].江苏农业学报,2023,(02):530.[doi:doi:10.3969/j.issn.1000-4440.2023.02.027]
　XU De-cong,WANG Yu,SHEN Zhang-jun,et al.Characteristics of achenes and seeds of Coreopsis lanceolata under different habitats[J].,2023,(05):530.[doi:doi:10.3969/j.issn.1000-4440.2023.02.027]

备注/Memo

备注/Memo:
收稿日期：2025-03-05基金项目：辽宁省教育厅2022年度高校基本科研项目(LJKQZ20222407)作者简介：孙忠戈(1990-),男,河北沧州人,硕士,讲师,主要从事花卉种质资源收集与植物营养研究。(E-mail)13889250030@163.com通讯作者：张伟,(E-mail)zwsxnd@sxau.edu.cn
更新日期/Last Update: 2025-06-24