[1]初龙,李伟,李欣亚,等.重金属超富集植物种子内生真菌多样性及其重金属抗性[J].江苏农业学报,2017,(01):43-49.[doi:10.3969/j.issn.1000-4440.2017.01.007 ]
　CHU Long,LI Wei,LI Xin ya,et al.Diversity and heavy metal resistance of endophytic fungi from seeds of hyperaccumulators[J].,2017,(01):43-49.[doi:10.3969/j.issn.1000-4440.2017.01.007 ]
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重金属超富集植物种子内生真菌多样性及其重金属抗性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年01期
页码:
43-49
栏目:
遗传育种·生理生化
出版日期:
2017-02-28

文章信息/Info

Title:
Diversity and heavy metal resistance of endophytic fungi from seeds of hyperaccumulators
作者:
初龙; 李伟; 李欣亚; 熊帜; 李海燕
（昆明理工大学生命科学与技术学院，云南昆明650500）
Author(s):
CHU Long; LI Wei; LI Xinya; XIONG Zhi; LI Haiyan
(Faculty of Life Sciences and Technology,Kunming University of Science and Technology, Kunming 650500, China)
关键词:
重金属超富集植物; 种子; 内生真菌; 多样性; 重金属抗性
Keywords:
hyperaccumulator; seed; endophytic fungi; diversity; heavy metal tolerance
分类号:
Q939.99
DOI:
10.3969/j.issn.1000-4440.2017.01.007
文献标志码:
A
摘要:
为探讨重金属超富集植物种子中内生真菌的种群组成及其在植物重金属耐受和富集方面可能发挥的生态学功能，通过分离纯化小花南芥（Arabis alpine）及土荆芥（Chenopodium ambrosioides）两种重金属超富集植物种子内生真菌，结合形态学特征和分子生物学数据对其进行鉴定，研究其多样性，并通过对内生真菌在含不同重金属培养基上生长速率的测定，研究其重金属抗性。结果显示：小花南芥和土荆芥两种重金属超富集植物种子中内生真菌的种类和数量均较少，存在绝对优势类群。两种植物中内生真菌的定殖率和多样性指数分别为740%、420%和0.78、135，远低于其他非极端环境植物内生菌的定殖率和多样性。重金属抗性测定结果表明，优势类群菌株的重金属抗性较其他类别突出，抗性菌株比例较高，个别菌株还表现为镉毒理兴奋型。通过最小抑制浓度检测发现镉毒理兴奋型菌株FXZ2可耐受40 mmol/L Pb2+、60 mmol/L Zn2+和220 mmol/L Cd2+。这些菌株可能在宿主植物抵御不良环境及重金属累积过程中发挥着重要作用。
Abstract:
To understand the population composition of endophytic fungi (EF) from hyperaccumulators seeds as well as their possible ecological role in host plants’ heavy metal (HM) tolerance and accumulation,the culturable EF from the seeds of two HM hyperaccumulators Arabis alpina and Chenopodium ambrosioides were isolated and further identified based on their morphological characteristics and molecular analysis.The HMs tolerance capacity was assessed through the growth rate measuring on PDA plates which contained different contents of HMs. The results showed that both the diversity and the colonization rate (CR) of EF in the seeds of two hyperaccumulators were lower, and the most dominant EF in them was different, too. The CR of A. alpine and C. ambrosioides were 74.0% and 42.0%, and the Shannon Index of them were 0.78 and 135, respectively. They were lower than those from the plant growing in nonextreme environment. HMs tolerance tests indicated that the isolates of dominant genera/species showed better HMs tolerance capacity than that of the other genera/species isolates (with higher ratio of resistant isolates), and some of them showed Cd toxicity excitatory. The result of minimum inhibitory concentration (MIC) tests indicated that the strain FXZ2 could tolerate lead, zinc and cadmium as high as 40 mmol/L, 60 mmol/L and 220 mmol/L, respectively. These strains may play an important role in improving host plants stress tolerance and affecting their HM accumulation.

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备注/Memo

备注/Memo:
收稿日期：2016-03-30 基金项目：国家自然科学基金项目（31360128、31560566） 作者简介：初龙（1989-），男，山东青岛人，硕士研究生，研究方向为内生真菌多样性及其生态学功能。（Tel）18213461423；（E-mail）769945807@qq.com。李伟为共同第一作者。 通讯作者：李海燕，（Tel）13888082968；（E-mail）13888082968@163.com
更新日期/Last Update: 2017-04-12