[1]段娅敏,王后苗,杨泽峰,等.基于转录组解析玉米苗期根系对非生物胁迫的响应[J].江苏农业学报,2025,(05):848-857.[doi:doi:10.3969/j.issn.1000-4440.2025.05.003]
　DUAN Yamin,WANG Houmiao,YANG Zefeng,et al.Transcriptome-based analysis of the response of maize root to abiotic stress at seedling stage[J].,2025,(05):848-857.[doi:doi:10.3969/j.issn.1000-4440.2025.05.003]
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基于转录组解析玉米苗期根系对非生物胁迫的响应()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年05期
页码:
848-857
栏目:
遗传育种·生理生化
出版日期:
2025-05-31

文章信息/Info

Title:
Transcriptome-based analysis of the response of maize root to abiotic stress at seedling stage
作者:
段娅敏; 王后苗; 杨泽峰; 徐辰武; 李鹏程; 王芸芸
(扬州大学农学院/江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室/江苏省作物基因组学与分子育种重点实验室/粮食作物现代产业协同创新中心,江苏扬州225009)
Author(s):
DUAN Yamin; WANG Houmiao; YANG Zefeng; XU Chenwu; LI Pengcheng; WANG Yunyun
(Agricultural College of Yangzhou University/Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics (Yangzhou University), Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou 225009, China)
关键词:
玉米; 非生物胁迫; 转录组学; 差异表达基因; 脱落酸
Keywords:
maize; abiotic stress; transcriptomics; differentially expressed genes; abscisic acid
分类号:
T513
DOI:
doi:10.3969/j.issn.1000-4440.2025.05.003
文献标志码:
A
摘要:
非生物胁迫已成为制约玉米产量和品质的关键因素之一。为了阐明玉米响应非生物胁迫的机制,本研究以玉米自交系B73为试验材料,设置了对照(CK)、干旱胁迫处理(D)、盐胁迫处理(S)以及干旱+盐复合胁迫处理(DS),对B73主胚根长进行动态监测,并对根系进行转录组测序。表型分析结果表明,与对照相比,卷苗后第3 d至第7 d不同胁迫处理主胚根长均显著变短。差异表达分析结果显示,在对照和不同胁迫处理比较组中共鉴定到1 526个差异表达基因。在对照与干旱胁迫、盐胁迫和干旱+盐复合胁迫比较组中分别鉴定到207个、170个和1 274个差异表达基因。功能富集结果表明,这些差异表达基因主要参与由脱落酸(ABA)合成和信号转导、转录因子介导的应对非生物胁迫的生物学过程。其中,MYB家族和ERF家族转录因子基因对非生物胁迫的响应最大。本研究结果为进一步揭示玉米幼苗响应非生物胁迫的分子机制奠定了基础。
Abstract:
Abiotic stresses (drought, salt) have become one of the key factors restricting the yield and quality of maize. To elucidate the mechanisms of maize responsed to abiotic stresses, in this study, B73 was used as the experimental material, and four treatments were set up: control (CK), drought stress treatment (D), salt stress treatment (S) and drought + salt compound stress treatment (DS). Primary root length was dynamically monitored, and the root transcriptome was sequenced. The results of phenotypic analysis showed that the primary root length was significantly shorter under different treatments compared with the control. A total of 1 526 differentially expressed genes were identified in the comparison groups between the control and different stress treatments. A total of 207, 170 and 1 274 differentially expressed genes were identified in the comparison groups between the control and drought stress, salt stress and drought + salt compound stress. Functional enrichment results suggested that these differentially expressed genes were mainly involved in the biological processes mediated by abscisic acid synthesis and signal transduction, and transcription factors in response to abiotic stress. MYB and ERF family transcription factor genes showed the greatest response to abiotic stress. The results of this study lay a foundation for further revealing the molecular mechanism of maize seedlings in response to abiotic stress.

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备注/Memo

备注/Memo:
收稿日期:2024-09-13基金项目:国家自然科学基金项目(32302654)作者简介:段娅敏(2000-),女,甘肃天水人,硕士研究生,主要从事玉米遗传育种研究。(E-mail)1446595629@qq.com通讯作者:王芸芸,(E-mail)008307@yzu.edu.cn
更新日期/Last Update: 2025-06-24