[1]张强,雍梁敏,代文婷,等.甜瓜掌状裂叶pll基因对贮藏期果实后熟生理的调控作用[J].江苏农业学报,2025,(04):756-763.[doi:doi:10.3969/j.issn.1000-4440.2025.04.014]
　ZHANG Qiang,YONG Liangmin,DAI Wenting,et al.Regulatory effect of the palmate-lobed leaf gene pll on the post-harvest physiology of melon fruits during storage[J].,2025,(04):756-763.[doi:doi:10.3969/j.issn.1000-4440.2025.04.014]
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甜瓜掌状裂叶pll基因对贮藏期果实后熟生理的调控作用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年04期
页码:
756-763
栏目:
园艺
出版日期:
2025-04-30

文章信息/Info

Title:
Regulatory effect of the palmate-lobed leaf gene pll on the post-harvest physiology of melon fruits during storage
作者:
张强1; 雍梁敏1; 代文婷2; 石云娇1
(1.海南经贸职业技术学院,海南 海口571127；2.海南省农业科学院农产品加工设计研究所,海南海口571100)
Author(s):
ZHANG Qiang1; YONG Liangmin1; DAI Wenting2; SHI Yunjiao1
(1.Hainan College of Economics and Business, Haikou 571127, China；2.Institute of Processing ＆ Design of Agroproducts, Hainan Academy of Agricultural Sciences, Haikou 571100, China)
关键词:
厚皮甜瓜; pll基因; 基因沉默; 酶活性; 后熟生理
Keywords:
muskmelon; pll gene; gene silencing; enzyme activity; post-harvest physiology
分类号:
S652.01
DOI:
doi:10.3969/j.issn.1000-4440.2025.04.014
文献标志码:
A
摘要:
甜瓜掌状裂叶pll基因属于珠被发育异常蛋白(ANT)转录因子基因,对植株生长、果实发育等具有调控作用。本研究使用病毒诱导的基因沉默(VIGS)技术对普通叶型甜瓜植株的pll基因进行基因沉默处理,对比在pll基因正常表达与沉默表达的情况下,甜瓜果实中脂氧合酶活性、乙烯释放与呼吸代谢的变化,并研究pll基因表达受到沉默抑制时,对果实中乙烯合成与糖代谢相关酶编码基因表达及相关酶活性产生的影响。结果表明,当果实中pll基因的表达水平较低时,能够降低脂氧合酶活性、乙烯释放量与呼吸速率,并且pll基因对果实中乙烯合成与糖代谢相关酶编码基因的表达有正向调控作用,当pll基因的表达受到沉默抑制时,果实中乙烯合成、糖代谢相关基因的相对表达量亦随之下调,从而降低乙烯合成与糖代谢相关酶的活性。由此可知,pll基因可能参与果实后熟生理的调控,并且pll相对表达量的下调对果实后熟生理代谢有延迟、抑制作用。
Abstract:
The melon palmate-lobed leaf gene pll belongs to AINTEGUMENTA （ANT) transcription factor gene, which plays an important role in regulating plant growth and fruit development. During the research, the gene silencing of pll was detected by virus-induced gene silencing (VIGS) technique. The changes of lipoxygenase activity, ethylene release and respiratory metabolism in fruits under normal and silent expression of pll gene were compared, and the effects of pll gene silencing inhibition on the expression of ethylene synthesis and sugar metabolism enzyme-encoding genes and related enzyme activities in fruits were studied. The results showed that the low expression of pll gene in fruits could reduce the activity of lipoxygenase, ethylene release and respiration rate. Moreover, the pll gene had a positive regulation effect on ethylene synthesis and carbohydrate metabolism gene expression in fruits, and when the expression of pll gene was suppressed by silencing, the expression of genes related to ethylene synthesis and glucose metabolism in fruits was also down-regulated, thus reducing the activities of ethylene synthesis and glucose metabolism enzymes. It can be seen that the pll gene is involved in the regulation of post-harvest physiology, and the down-regulation of pll gene expression has a delayed and inhibitory effect on the post-harvest physiological metabolism of melon fruits.

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备注/Memo

备注/Memo:
收稿日期:2024-10-29基金项目:海南省教育厅科研项目(Hnky2024-75)；海南经贸职业技术学院高层次人才引进项目(hnjmk2022303)作者简介:张强(1977-),男,安徽界首人,博士,副研究员,研究方向为果蔬贮藏保鲜。(E-mail)821861127@qq.com通讯作者:石云娇,(E-mail)yunjiaoshi009@163.com
更新日期/Last Update: 2025-05-26