[1]娄丽娜,羊杏平,朱凌丽,等.基于BSA-seq 技术定位黄瓜黄绿叶色突变基因[J].江苏农业学报,2024,(09):1711-1718.[doi:doi:10.3969/j.issn.1000-4440.2024.09.015]
　LOU Lina,YANG Xingping,ZHU Lingli,et al.Mapping of cucumber virescent yellow leaf mutant gene based on BSA-seq technology[J].,2024,(09):1711-1718.[doi:doi:10.3969/j.issn.1000-4440.2024.09.015]
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基于BSA-seq 技术定位黄瓜黄绿叶色突变基因()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年09期
页码:
1711-1718
栏目:
园艺
出版日期:
2024-09-30

文章信息/Info

Title:
Mapping of cucumber virescent yellow leaf mutant gene based on BSA-seq technology
作者:
娄丽娜; 羊杏平; 朱凌丽; 姚协丰; 徐建; 张曼; 刘广; 侯茜; 刘金秋; 徐锦华
(江苏省农业科学院蔬菜研究所/江苏省高效园艺作物遗传改良重点实验室,江苏南京210014)
Author(s):
LOU Lina; YANG Xingping; ZHU Lingli; YAO Xiefeng; XU Jian; ZHANG Man; LIU Guang; HOU Qian; LIU Jinqiu; XU Jinhua
(Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China)
关键词:
黄瓜; 黄绿叶色突变体; BSA-seq; 基因定位
Keywords:
cucumber; virescent yellow leaf mutant; BSA-seq; gene mapping
分类号:
S642.2
DOI:
doi:10.3969/j.issn.1000-4440.2024.09.015
文献标志码:
A
摘要:
为明确课题组从地方收集的黄瓜黄绿叶色突变体中黄绿叶色突变基因,本研究利用绿叶(野生型)与黄绿叶色(突变体)黄瓜材料为亲本,配制正反杂交一代及二代分离群体,在分析黄绿叶色突变基因遗传规律的基础上,利用BSA-seq技术对双亲及F2代叶色极端混池进行测序,筛选黄瓜黄绿叶色突变性状的关联标记及候选基因。结果表明，绿叶色对黄绿叶色为完全显性,黄绿叶色突变基因为隐性基因。突变性状关联分析(SNP-index检测)得到1个与黄绿叶色突变相关的候选区域,位于黄瓜1号染色体上,总长度18 484 bp。该候选区域共有118个与黄绿叶色突变相关的SNP位点；候选区域共注释到3个基因,其中CsaV3_1G032820基因为黄瓜黄绿叶色突变最可能相关基因。本研究结果可为黄瓜黄绿叶色突变基因克隆和分子标记辅助育种提供基础。
Abstract:
In order to clarify the virescent yellow leaf mutant gene in cucumber virescent yellow leaf mutant collected by the research group from the local area, this study used green leaf (wild type) and virescent yellow leaf (mutant) cucumber materials as parents to prepare a reciprocal hybrid generation and a second generation separation population. On the basis of analyzing the genetic law of virescent yellow leaf mutant genes, BSA-seq technology was used to sequence the extreme mixing pools of parents and F2 generations, and to screen the associated markers and candidate genes of cucumber virescent yellow leaf mutant traits. The results showed that the green leaf color was completely dominant to virescent yellow leaf color, and the virescent yellow leaf color mutant gene was a recessive gene. A candidate region associated with virescent yellow leaf color mutation was obtained by SNP-index detection, which was located on chromosome 1 of cucumber, with a total length of 18 484 bp. A total of 118 SNP loci associated with virescent yellow leaf color mutation were found in this candidate region. Three genes were annotated in the candidate region, among which CsaV3_1G032820 gene was the most likely related gene of cucumber virescent yellow leaf color mutation. The results of this study can provide a basis for cucumber virescent yellow leaf color mutant gene cloning and molecular marker-assisted breeding.

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备注/Memo

备注/Memo:
收稿日期：2023-09-13基金项目：江苏省种业振兴揭榜挂帅项目[JBGS(2021)018]作者简介：娄丽娜(1982-),女,河南濮阳人,博士,研究员,主要从事瓜类作物遗传育种研究。(Tel)025-84391221；(E-mail)linabeibei@163.com通讯作者：徐锦华,(Tel) 025-84390264;(E-mail)jinhuaxu@jaas.ac.cn
更新日期/Last Update: 2024-11-17