[1]潘娇艳,肖世伟,夏瑾,等.绿色草莓再生体系的建立[J].江苏农业学报,2024,(01):174-182.[doi:doi:10.3969/j.issn.1000-4440.2024.01.019]
　PAN Jiao-yan,XIAO Shi-wei,XIA Jin,et al.The establishment of regeneration system of Fragaria viridis Weston[J].,2024,(01):174-182.[doi:doi:10.3969/j.issn.1000-4440.2024.01.019]
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绿色草莓再生体系的建立()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年01期
页码:
174-182
栏目:
园艺
出版日期:
2024-01-30

文章信息/Info

Title:
The establishment of regeneration system of Fragaria viridis Weston
作者:
潘娇艳1; 肖世伟1; 夏瑾2; 倪知游1; 邹雨婷1; 乔玉山1; 2
(1.南京农业大学园艺学院,江苏南京210095；2.江苏省农业科学院果树研究所/江苏省高效园艺作物遗传改良重点实验室,江苏南京210014)
Author(s):
PAN Jiao-yan1; XIAO Shi-wei1; XIA Jin2; NI Zhi-you1; ZOU Yu-ting1; QIAO Yu-shan1; 2
(1.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;2.Institute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China)
关键词:
绿色草莓; 愈伤组织; 再生; 遗传转化体系
Keywords:
Fragaria viridis Weston; callus; regeneration;  genetic transformation system
分类号:
S668.4
DOI:
doi:10.3969/j.issn.1000-4440.2024.01.019
文献标志码:
A
摘要:
建立高效、稳定的绿色草莓再生体系是构建其遗传转化体系的基础。本研究以绿色草莓匍匐茎茎尖为外植体,置于不同培养基中进行初代培养获得试管苗。在含有不同配比植物生长调节剂的培养基中对叶龄为30 d的试管苗叶盘进行脱分化处理。以叶盘诱导产生的愈伤组织为试验材料,探究不同植物生长调节剂对愈伤组织产生不定芽的影响。结果表明,最适用于绿色草莓初代培养的培养基是MS+0.2 mg/L 6-苄氨基嘌呤(6-BA)+0.2 mg/L 赤霉素(GA3),在此培养基中绿色草莓离体茎尖成活率最高,生长状况最好；诱导叶盘脱分化、愈伤组织再分化和诱导不定芽生根的适宜培养基分别是MS+1.5 mg/L 2,4-二氯苯氧乙酸(2,4-D)+2.0 mg/L 噻苯隆(TDZ)、1/2 MS+1.5 mg/L 6-BA+0.1 mg/L吲哚丁酸(IBA)和1/2 MS+0.2 mg/L IBA+0.1 mg/L吲哚乙酸(IAA),其出愈率、再分化率和生根率分别为100%、91.0%、100%。本研究建立了以绿色草莓匍匐茎茎尖为试验材料获得试管苗的组织快繁体系和以叶盘为外植体经脱分化、愈伤组织再分化2个阶段的再生体系,为建立其遗传转化体系奠定了技术基础。
Abstract:
The establishment of an efficient and stable regeneration system of Fragaria viridis Weston is the basis of its genetic transformation system. In this study, the stolon tips of Fragaria viridis Weston were used as explants and placed in different media for primary culture to obtain test-tube seedlings. The leaf discs of test-tube plantlets with leaf age of 30 days were dedifferentiated in the medium containing different ratios of plant growth regulators. The calluses induced by leaf discs were used as materials to explore the effects of different plant growth regulators on adventitious buds. The results showed that the most suitable medium for primary culture of Fragaria viridis Weston was MS+0.2 mg/L N-(phenylmethyl)-9H-purin-6-amine (6-BA)+0.2 mg/L gibberellin A3 (GA3). In this medium, the survival rate of Fragaria viridis Weston shoot tips in vitro was the highest and the growth condition was the best. The suitable media for inducing leaf discs dedifferentiation, callus redifferentiation and adventitious bud rooting were MS+1.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D )+2.0 mg/L thidiazuron (TDZ), 1/2 MS+1.5 mg/L 6-BA+0.1 mg/L 3-indolebutyric acid (IBA) and 1/2 MS+0.2 mg/L IBA+0.1 mg/L 3-indoleacetic acid (IAA), respectively. The callus incidence, redifferentiation rate and rooting rate were 100%, 91.0% and 100%, respectively. In this study, the tissue rapid propagation system using the stolon tips of Fragaria viridis Weston as experimental materials and a regeneration system using leaf discs as explants through dedifferentiation and callus redifferentiation were established, which laid a technical foundation for the establishment of its genetic transformation system.

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备注/Memo

备注/Memo:
收稿日期：2023-02-24基金项目：国家自然科学基金项目(32072540)；江苏省农业科技自主创新基金项目[CX(21)2019]作者简介：潘娇艳(1998-),女,广西贺州人,硕士研究生,主要从事草莓分子生物学研究。(E-mail)1853262521@qq.com通讯作者：乔玉山,(E-mail)qiaoyushan@njau.edu.cn
更新日期/Last Update: 2024-03-17