[1]钱蕾,秦毅,张佳,等.滞育时间对豆天蛾幼虫营养成分及代谢酶活性的影响[J].江苏农业学报,2024,(01):149-155.[doi:doi:10.3969/j.issn.1000-4440.2024.01.016]
　QIAN Lei,QIN Yi,ZHANG Jia,et al.Effects of diapause time on nutritional components and metabolic enzymes activities in Clanis bilineata tsingtauica larvae[J].,2024,(01):149-155.[doi:doi:10.3969/j.issn.1000-4440.2024.01.016]
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滞育时间对豆天蛾幼虫营养成分及代谢酶活性的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年01期
页码:
149-155
栏目:
畜牧兽医·水产养殖·益虫饲养
出版日期:
2024-01-30

文章信息/Info

Title:
Effects of diapause time on nutritional components and metabolic enzymes activities in Clanis bilineata tsingtauica larvae
作者:
钱蕾1; 秦毅1; 张佳2; 李宗男1; 邓盼1; 周刚3; 李情怡4; 廖怀建1; 4
(1.江苏省农业科学院休闲农业研究所,江苏南京210014；2.扬州大学植物保护学院,江苏扬州225009；3.淮安市农业科学院,徐淮地区淮阴农业科学研究所,江苏淮安223001；4.江苏科技大学生物技术学院,江苏镇江212100)
Author(s):
QIAN Lei1; QIN Yi1; ZHANG Jia2; LI Zong-nan1; DENG Pan1; ZHOU Gang3; LI Qing-yi4; LIAO Huai-jian1; 4
(1.Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.College of Plant Protection, Yangzhou University, Yangzhou 225009, China;3.Huaian Academy of Agricultural Sciences, Huaiyin Institute of Agricultural Sciences of the Xuhuai District of Jiangsu Province, Huaian 223001, China;4.College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China)
关键词:
豆天蛾; 滞育; 代谢酶; 蛋白质; 氨基酸; 脂肪酸
Keywords:
Clanis bilineata tsingtauica; diapause; metabolic enzymes; protein; amino acids; fatty acids
分类号:
S899.9
DOI:
doi:10.3969/j.issn.1000-4440.2024.01.016
文献标志码:
A
摘要:
为了明确不同滞育时间对豆天蛾幼虫的影响,本研究测定了豆天蛾幼虫体内营养物质(包括蛋白质、氨基酸和脂肪酸)含量及肠道代谢酶活性的变化。结果表明,不同滞育时间的豆天蛾幼虫体内蛋白质含量均显著高于对照,且在滞育后期(150 d)达到最高值。滞育150 d的豆天蛾幼虫体内18种氨基酸含量均显著高于对照。与对照相比,滞育60 d豆天蛾幼虫体内亚油酸含量降低0.030个百分点(P＜0.05),滞育30 d、90 d、120 d、150 d豆天蛾幼虫体内亚麻酸含量分别高于对照0.140个百分点、0.060个百分点、0.060个百分点、0.080个百分点(P＜0.05)。滞育30 d豆天蛾幼虫肠道碱性磷酸酶、己糖激酶活性显著低于对照(P＜0.05),滞育120 d时达到最高值；海藻糖酶、丙酮酸激酶活性在滞育90 d达到最高值；滞育120 d山梨醇脱氢酶活性最高,比对照高18.37%(P＜0.05)。综上所述,滞育期的豆天蛾幼虫营养价值提高,代谢酶活性波动明显。
Abstract:
To clarify the effects of diapause time on Clanis bilineata tsingtauica larvae, the contents of nutrients (including protein, amino acids, and fatty acids) and the activities of intestinal metabolic enzymes in the larvae were measured. The results showed that the protein content in diapause larvae was significantly higher than that in control, and reached the highest value in the late diapause period (150 d). The contents of 18 amino acids at 150 days of diapause were significantly higher than those of the control. Compared with the control, the content of linoleic acid in the larvae of Clanis bilineata tsingtauica decreased by 0.030 percentage points (P＜0.05) on the 60th day of diapause. At 30 d, 90 d, 120 d and 150 d of diapause, the content of linolenic acid in the larvae was 0.140 percentage points, 0.060 percentage points, 0.060 percentage points and 0.080 percentage points higher than that of the control (P＜0.05). The activities of alkaline phosphatase and hexokinase in the intestine of the larvae at 30 days of diapause were significantly lower than those of the control, and reached the highest value at 120 days of diapause. The activities of trehalase and pyruvate kinase reached the highest value at 90 days of diapause. The activity of sorbitol dehydrogenase was the highest at 120 days of diapause, which was 18.37% higher than that of the control(P＜0.05). In summary, the nutritional value of the diapause larvae was improved, and the activity of metabolic enzymes fluctuated significantly.

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备注/Memo

备注/Memo:
收稿日期:2023-07-05基金项目:国家自然科学基金项目(32100398)；江苏省自然科学基金项目(BK20231393)；淮安市科技项目(HAN202206)作者简介:钱蕾(1992-),女,江苏沭阳人,博士,助理研究员,主要从事资源昆虫功能物质开发与利用研究。(E-mail)qianlei14930@sina.cn通讯作者:廖怀建,(E-mail)lhj@jaas.ac.cn
更新日期/Last Update: 2024-03-17