[1]张雪莲,罗德旭,杨红,等.外源褪黑素和硒对高温胁迫下辣椒生理特性和抗氧化系统的影响[J].江苏农业学报,2023,(08):1729-1738.[doi:doi:10.3969/j.issn.1000-4440.2023.08.013]
　ZHANG Xue-lian,LUO De-xu,YANG Hong,et al.Effects of exogenous melatonin and selenium on physiological properties and antioxidant systems of chilies under high temperature stress[J].,2023,(08):1729-1738.[doi:doi:10.3969/j.issn.1000-4440.2023.08.013]
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外源褪黑素和硒对高温胁迫下辣椒生理特性和抗氧化系统的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年08期
页码:
1729-1738
栏目:
园艺
出版日期:
2023-12-31

文章信息/Info

Title:
Effects of exogenous melatonin and selenium on physiological properties and antioxidant systems of chilies under high temperature stress
作者:
张雪莲1; 罗德旭1; 杨红1; 刁卫平2; 王玮玮1; 孙玉东1; 白甜1; 刘璐1; 尹莲1
(1.江苏徐淮地区淮阴农业科学研究所,江苏淮安223001；2.江苏省农业科学院,江苏南京210014)
Author(s):
ZHANG Xue-lian1; LUO De-xu1; YANG Hong1; DIAO Wei-ping2; WANG Wei-wei1; SUN Yu-dong1; BAI Tian1; LIU Lu1; YIN Lian1
(1.Huaiyin Institute of Agricultural Sciences of the Xuhuai District of Jiangsu Province, Huaian 223001, China；2.Jiangsu Academy of Agaricultural Sciences, Nanjing 210014, China)
关键词:
辣椒; 褪黑素; 硒; 高温胁迫; 抗氧化系统
Keywords:
pepper; melatonin; selenium; high temperature stress; antioxidant system
分类号:
S641.306
DOI:
doi:10.3969/j.issn.1000-4440.2023.08.013
文献标志码:
A
摘要:
本研究旨在探究外源褪黑素(Melatonin,MT)和硒(Se)对高温胁迫下辣椒的生理特性和抗氧化系统的影响。以耐热品系R9和育成品种淮红一号为供试材料,在6～7片真叶期施用不同含量的褪黑素和Na2SeO3,以常温清水处理(CK1)和高温清水处理(CK2)为对照。于花期测定花粉活力、热害指数、叶片相对电导率、抗氧化酶(SOD、POD、APX)活性和MDA含量。于果实成熟期测定植株和果实性状以及地上部分和果实的鲜质量、干质量。结果表明:在高温胁迫下,单施0.6 mg/kg Na2SeO3、单施150 μmol/L褪黑素、0.6 mg/kg Na2SeO3和150 μmol/L褪黑素配施3个处理均能明显减少细胞质膜透性，提高花粉萌发率、抗氧化酶活性和叶绿素含量,增加果实产量及干物质积累量,其中同时施用0.6 mg/kg Na2SeO3和150 μmol/L褪黑素处理效果最好。本研究可为硒和褪黑素在植物抵御高温胁迫上的应用提供理论依据和技术参考。
Abstract:
This study was designed to investigate the effects of exogenous melatonin (MT) and selenium (Se) on the physiological properties and antioxidant systems of chilies under high temperature stress. Using heat-resistant line R9 and cultivated variety Huaihong No.1 as test materials, different contents of melatonin and Na2SeO3 were applied during the 6-7 true leaf stage. Pollen vitality, heat damage index, relative conductivity of leaves, activities of antioxidant enzymes (SOD, POD, APX), and MDA content were measured during the flowering period with ordinary temperature water treatment (CK1) and high temperature water treatment (CK2) as controls. The plant traits, fruit traits, as well as the fresh and dry weight of the aboveground parts and fruits were measured during the fruit ripening period. The results showed that under high temperature stress, single application of 0.6 mg/kg Na2SeO3 treatment, single application of 150 μmol/L melatonin, combined application of 0.6 mg/kg Na2SeO3 and 150 μmol/L melatonin could significantly reduce plasma membrane permeability, increase pollen germination rate, antioxidant enzyme activity, chlorophyll content, fruit yield and dry matter accumulation. The combined application of 0.6 mg/kg Na2SeO3 and 150 μmol/L melatonin had the best effect. This study can provide theoretical basis and technical reference for the application of selenium and melatonin in plant resistance to high temperature stress.

参考文献/References:

[1]邹学校,胡博文,熊程,等. 中国辣椒育种60年回顾与展望[J]. 园艺学报,2022,49(10):2099-2118.
[2]HUANG L J,CHENG G X,KHAN A,et al. CaHSP16.4 a small heat shock protein gene in pepper, is involved in heat and drought tolerance[J]. Protoplasma,2019,256:39-51.
[3]WANG X,ZHAO C,MLLER C,et al. Emergent constraint on crop yield response to warmer temperature from field experiments[J]. Nature Sustainability,2020,3:908-916.
[4]余楚英,尹延旭,王飞,等. 茄果类蔬菜热胁迫及耐热性研究进展[J]. 中国蔬菜,2021(4):27-29.
[5]王静,梁成亮,张西露,等. 辣椒种质资源的耐热性评价与鉴定[J]. 湖南农业大学学报(自然科学版),2020,46(5):551-552.
[6]高素燕,吕敬刚,刘文明. 辣椒高温胁迫和耐热机理的研究进展[J]. 天津农业科学,2012,18(1):31-34.
[7]何铁光,董文斌,王爱勤,等. 高温胁迫下辣椒生理生化响应机理初步探讨[J]. 西南农业学报,2013,26(2):543-544.
[8]余楚英,尹延旭,王飞,等. 茄果类蔬菜热胁迫及耐热性研究进展[J]. 中国蔬菜,2021(4):27-40.
[9]余雪娜,李婧娴,谢永东,等. 外源褪黑素对高温胁迫下茄子幼苗光合及抗氧化特性的影响[J]. 湖南农业大学学报(自然科学版),2016,42(5):496-497.
[10]徐宁,曹娜,王闯,等. 外源褪黑素对硝酸盐胁迫下番茄幼苗抗氧化系统的影响[J]. 北方园艺,2019(3):1-5.
[11]徐向东,孙艳,孙波,等. 高温胁迫下外源褪黑素对黄瓜幼苗活性氧代谢的影响[J]. 应用生态学报,2010,21(5):1295-1296.
[12]BALAL R M,SHAHID M A,JAVAID M M,et al. The role of selenium in amelioration of heat-induced oxidative damage in cucumber under high temperature stress[J]. Acta Physiol Plant,2016,38(6):158.
[13]尚庆茂,陈淑芳,张志刚. 硒对高温胁迫下辣椒叶片抗氧化酶活性的调节作用[J]. 园艺学报,2005,32(1):35-36.
[14]尚庆茂,陈淑芳,张志刚. 硒对高温逆境下辣椒光合特性的影响[C]//中国园艺学会. 中国园艺学会第六届青年学术讨论会论文集. 西安:陕西科学技术出版社,2004:625-630.
[15]姜燕,黄新根,杨寅桂,等. 辣椒苗期耐热性及其生理生化特性研究[J]. 长江蔬菜,2014(12):47-50.
[16]张子学,孙峰. 辣椒花粉生活力最佳测定方法的筛选[J]. 种子,2002(1):32-33.
[17]杨少瑕,吕庆芳,梁春林. 电导法对32个辣椒品种组合抗寒性的测定及配合力分析[J]. 黑龙江农业科学,2014(2):6-15.
[18]余小兰,张静,李光义,等. 辣椒在不同施硒水平下硒累积规律[J]. 热带作物学报,2021,42(7):1988-1990.
[19]赵娜,孙艳,王德玉,等. 外源褪黑素对高温胁迫条件下黄瓜幼苗氮代谢的影响[J]. 植物生理学报,2012,48(6):557.
[20]MURCH S J,SIMMONS C B,SAXENA P K. Melatonin in feverfew and other medicinal plants[J]. Lancet,1997,350 :1598-1599.
[21]张贵友,李萍,戴尧仁. 低温胁迫下褪黑激素对烟草悬浮细胞精氨酸脱羧酶活性的影响[J]. 植物学通报,2005,22(5):555-559.
[22]魏茜雅,林欣琪,梁腊梅,等. 褪黑素引发处理提高朝天椒种子萌发及幼苗耐盐性的生理机制[J]. 江苏农业学报,2022,38(6):1637-1647.
[23]郭爱华. 外源褪黑素对盐胁迫下苦菜幼苗生长的影响[J]. 江苏农业科学,2022,50(13):153-157.
[24]高安静,刘婷婷,周美亮,等. 外源褪黑素对干旱胁迫下苦荞幼苗生长及生理特性的影响[J]. 南方农业学报,2021,52(11):3003-3012.
[25]叶欣悦,闫见敏,杨雪莲,等. SlCOMT1基因克隆及在番茄组织器官中的表达和褪黑素生物合成变化[J]. 江苏农业科学,2022,50(23):49-54.
[26]徐向东,孙艳,孙波,等. 高温胁迫下外源褪黑素对黄瓜幼苗活性氧代谢的影响[J]. 应用生态学报,2010,21(5):1295-1300.
[27]崔婉宁,杨金荣,刘梦梦,等. 褪黑素对半夏高温倒苗及DNA甲基化的影响[J]. 农业生物技术学报,2023,31(3):500-508.
[28]王博伟,陈艳丽,朱国鹏,等. 叶面喷施褪黑素对海南高温季节水培叶用莴苣生长生理的影响[J]. 中国蔬菜,2022(11):80-85.
[29]丁东霞,李能慧,李静,等. 外源褪黑素对低温弱光胁迫下辣椒叶绿素荧光和抗氧化系统的影响[J]. 浙江农业学报,2022,34(9):1935-1944.
[30]陈淑芳. 硒对辣椒幼苗生长发育及耐热性的影响[D]. 晋中:山西农业大学,2003.
[31]薛泰麟,侯少范,谭见安,等. 硒在高等植物体内的抗氧化作用 Ⅰ. 硒对过氧化作用的抑制效应及酶促机制的探讨[J]. 科学通报,1993(3):274-277.
[32]罗盛国,刘元英,姜伯文,等. 硒对棚室黄瓜生物抗氧化能力的影响[J]. 北方园艺,2000(3):10-11.
[33]尚庆茂,李平兰. 硒在高等植物中的生理作用[J]. 植物生理学通讯,1998(4):284-288.
[34]DJANAGIHRAMAN M,BELLIRAJ N,BOSSMANN S H,et al. High-temperature stress alleviation by selenium nanoparticle treatment in grain sorghum [J]. ACS Omega,2018,3(3):2479-2491.
[35]AKLADIOUS S A. Influencen of different soaking times with seleniumon growth,metabolic activities of wheat seedlings under low temperature stress [J]. African Journal of Biotechnology,2012,11(82):14792-14804.
[36]陈思蒙,李子玮,张璐翔,等. 硒在植物抵御胁迫中作用的研究进展[J]. 中国农业科技导报,2020,22(3):6-13.

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备注/Memo

备注/Memo:
收稿日期:2023-04-12基金项目:淮安市农业科学研究院科研发展基金项目(HNY201906)；江苏省种业振兴“揭榜挂帅”项目[JBGS(2021)065]作者简介:张雪莲(1996-),女,江苏镇江人,硕士,研究实习员,主要从事蔬菜栽培及育种研究。(E-mail)xuelianzhang2022@163.com通讯作者:罗德旭,(E-mail)498782025@qq.com
更新日期/Last Update: 2024-01-15