[1]赵胡,沈程,郝庆萍,等.香椿中3个水通道蛋白基因(TsAQP)的克隆和表达分析[J].江苏农业学报,2023,(08):1635-1645.[doi:doi:10.3969/j.issn.1000-4440.2023.08.003]
 ZHAO Hu,SHEN Cheng,HAO Qing-ping,et al.Cloning and expression analysis of three aquaporin genes (TsAQP) in Toona sinensis[J].,2023,(08):1635-1645.[doi:doi:10.3969/j.issn.1000-4440.2023.08.003]
点击复制

香椿中3个水通道蛋白基因(TsAQP)的克隆和表达分析()
分享到:

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年08期
页码:
1635-1645
栏目:
遗传育种·生理生化
出版日期:
2023-12-31

文章信息/Info

Title:
Cloning and expression analysis of three aquaporin genes (TsAQP) in Toona sinensis
作者:
赵胡12沈程12郝庆萍12刘艳红3季春艳3石潇瀑1
(1.阜阳师范大学生物与食品工程学院,安徽阜阳236037;2.抗衰老中草药安徽省工程技术研究中心,安徽阜阳236037;3.阜阳师范大学信息工程学院,安徽阜阳236041)
Author(s):
ZHAO Hu12SHEN Cheng12HAO Qing-ping12LIU Yan-hong3JI Chun-yan3SHI Xiao-pu1
(1.Biology and Food Engineering College, Fuyang Normal University, Fuyang 236037, China;2.Engineering Technology Research Center of Anti-aging Chinese Herb, Fuyang 236037, China;3.College of Information Engineering, Fuyang Normal University, Fuyang 236041, China)
关键词:
香椿水通道蛋白基因克隆
Keywords:
Toona sinensisaquaporingene cloning
分类号:
S644.4
DOI:
doi:10.3969/j.issn.1000-4440.2023.08.003
文献标志码:
A
摘要:
水通道蛋白(Aquaporins,AQP)能够促进水分和小分子物质在膜上的运输,维持细胞水分平衡。本研究利用香椿转录组数据,克隆到3个香椿水通道蛋白基因(TsAQP),即TsPIP1-5(GenBank登录号MT501152)、TsPIP2-5(GenBank登录号MT501153)和TsTIP2-1(GenBank登录号MT501154)。利用生物信息学和实时荧光定量PCR(qRT-PCR)技术,结合生理表型分析,研究TsPIP1-5、TsPIP2-5和TsTIP2-1蛋白性质、表达模式和在香椿芽采后贮藏中的功能。结果表明:从转录组数据中鉴定出的20个TsAQP可分为5类;TsPIP1-5、TsPIP2-5和TsTIP2-1是通过跨膜区定位于膜的疏水性稳定蛋白,含磷酸化位点,无信号肽;TsPIP1-5、TsPIP2-5和TsTIP2-1在根、茎和叶中均有表达且有一定的器官差异性;TsPIP1-5、TsPIP2-5和TsTIP2-1在香椿芽低温贮藏过程中表达量提高,暗示TsAQP在香椿芽采后脱水过程中有调控作用。本研究结果为进一步研究香椿中TsAQP基因的功能和作用机制奠定了基础。
Abstract:
Aquaporins (AQP) facilitate the transport of water and small molecules across membranes and maintain water balance of cells. Three aquaporin genes in Toona sinensis (TsAQP), TsPIP1-5(GenBank accession number MT501152), TsPIP2-5(GenBank accession number MT501153), and TsTIP2-1(GenBank accession number MT501154), were cloned based on transcriptome sequencing. Using a combination of bioinformatics and real-time fluorescence quantitative PCR (qRT-PCR), we studied the physiological phenotypes, protein properties, expression patterns, and function of TsPIP1-5, TsPIP2-5 and TsTIP2-1 during post harvest storage of Toona sinensis buds. The results indicated that 20 TsAQP proteins identified from the transcriptome data could be classified into five subfamilies. TsPIP1-5, TsPIP2-5 and TsTIP2-1 were hydrophobic stable proteins located on the membrane through the transmembrane region, containing phosphorylation sites and no signal peptide. Different expression levels of TsPIP1-5, TsPIP2-5, and TsTIP2-1 were detected in the roots, stems and leaves with certain organ differences. The expression levels of TsPIP1-5, TsPIP2-5, and TsTIP2-1 increased during cold storage of Toona sinensis buds, suggesting that they played a regulatory role in the process of post harvest dehydration. The results of this study lay a foundation for further research on function and regulatory mechanisms of TsAQPs in Toona sinensis.

参考文献/References:

[1]MAUREL C, BOURSIAC Y, LUU D, et al. Aquaporins in plants [J]. Physiological Reviews,2015,95(4):1321-1358.
[2]BYRT C S, ZHAO M, KOURGHI M, et al. Non-selective cation channel activity of aquaporin AtPIP2;1 regulated by Ca2+ and pH [J]. Plant, Cell & Environment,2017,40(6):802-815.
[3]KOURGHI M, NOURMOHAMMANDI S, PEI J V, et al. Divalent cations regulate the ion conductance properties of diverse classes of aquaporins [J]. International Journal of Molecular Sciences,2017,18:2323.
[4]FUJIYOSHI Y, MITSUOKA K, DE-GROOT B L, et al. Structure and function of water channels [J]. Current Opinion in Structural Biology,2002,12(4):509-515.
[5]TRNROTH-HORSEFIELD S, WANG Y, HEDFALK K, et al. Structural mechanism of plant aquaporin gating [J]. Nature,2006,439(7077):688-694.
[6]MAUREL C, VERDOUCQ L, LUU D, et al. Plant aquaporins:membrane channels with multiple integrated functions [J]. Annual Review of Plant Biology,2008,59(1):595-624.
[7]ANDERBERG H I, DANIELSON J , JOHANSON U. Algal MIPs, high diversity and conserved motifs [J]. BMC Evolutionary Biology,2011,11:110.
[8]ANDERBERG H, KJELLBOM P, JOHANSON U. Annotation of selaginella moellendorffii major intrinsic proteins and the evolution of the protein family in terrestrial plants [J]. Frontiers in Plant Science,2012,3:33.
[9]FRANSSEN H J, NAP J P, BISSELING T. Biological nitrogen fixation [M]. New York:Chapman & Hall,1992:598-624.
[10]WEAVER C D, SHOMER N H, LOUIS C F, et al. Nodulin 26, a nodule-specific symbiosome membrane protein from soybean, is an ion channel [J]. Journal of Biological Chemistry,1994,269(27):17858-17862.
[11]MA J F, TAMAI K, YAMAJI N, et al. A silicon transporter in rice [J]. Nature,2006,440(7084):688-691.
[12]TAKANO J, WADA M, LUDEWIG U, et al. The arabidopsis major intrinsic protein NIP5;1 is essential for efficient boron uptake and plant development under boron limitation [J]. The Plant Cell,2006,18(6):1498-1509.
[13]MIZUTANI M, WATANABE S, NAKAGAWA T, et al. Aquaporin NIP2;1 is mainly localized to the Er membrane and shows root-specific accumulation in Arabidopsis thaliana [J]. Plant and Cell Physiology,2006,47(10):1420-1426.
[14]ISHIKAWA F, SUGA S, UEMURA T, et al. Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana [J]. FEBS Letters,2005,579(25):5814-5820.
[15]BIENERT G P, BIENERT M D, JAHN T P, et al. Solanaceae XIPs are plasma membrane aquaporins that facilitate the transport of many uncharged substrates[J]. The Plant Journal,2011,66(2):306-317.
[16]LI G, SANTONI V, MAUREL C. Plant aquaporins:Roles in plant physiology [J]. Biochimica et Biophysica Acta (BBA)-General Subjects,2014,1840(5):1574-1582.
[17]DANIELSON J , JOHANSON U. Unexpected complexity of the aquaporin gene family in the moss physcomitrella patens [J]. BMC Plant Biology,2008,8(1):45.
[18]JOHANSON U, GUSTAVSSON S. A new subfamily of major intrinsic proteins in plants [J]. Molecular Biology and Evolution,2002,19:456-461.
[19]NORONHA H, AGASSE A, MARTINS A P, et al. The grape aquaporin VvSIP1 transports water across the ER membrane [J]. Journal of Experimental Botany,2014,65(4):981-993.
[20]王文铖,崔克辉. 非生物逆境对植物水孔蛋白表达调控的研究进展[J]. 植物生理学报,2016,52(4):423-430.
[21]KAMMERLOHER W, FISCHER U, PIECHOTTKA G P, et al. Water channels in the plant plasma membrane cloned by immunoselection from a mammalian expression system [J]. The Plant Journal,1994,6(2):187-199.
[22]CHAUMONT F O, BARRIEU F O, WOJCIK E, et al. Aquaporins constitute a large and highly divergent protein family in maize1 [J]. Plant Physiology,2001,125(3):1206-1215.
[23]JOHANSON U, KARLSSON M, JOHANSSON I, et al. The complete set of genes encoding major intrinsic proteins in arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants [J]. Plant Physiology,2001,126(4):1358-1369.
[24]CHAUMONT F, TYERMAN S D. Aquaporins:Highly regulated channels controlling plant water relations [J]. Plant Physiology,2014,164(4):1600-1618.
[25]邢小艺,孙芳旭,郭轩佑. 香椿的综合利用价值浅析[J]. 现代园艺,2014,268(16):222.
[26]杨慧,毛维林,赵守涣,等. 冰温结合开孔调湿包装对香椿嫩芽的保鲜效应[J]. 食品与机械,2017,33(9):121-125,140.
[27]WANG X, GAO F, BING J, et al. Overexpression of the Jojoba aquaporin gene, ScPIP1, enhances drought and salt tolerance in transgenic Arabidopsis [J]. International Journal of Molecular Sciences,2019,20:153.
[28]HACHEZ C, ZELAZNY E, CHAUMONT F. Modulating the expression of aquaporin genes in planta:A key to understand their physiological functions? [J]. Biochimica et Biophysica Acta (BBA)-Biomembranes,2006,1758(8):1142-1156.
[29]GUO A H, HAO J F, SU Y, et al. Two aquaporin gnes, GhPIP2;7 and GhTIP2;1, positively regulate the tolerance of upland cotton to salt and osmotic stresses [J]. Frontiers in Plant Science,2022,12:780486.
[30]SAITOU N, NEI M. The neighbor-joining method:A new method for reconstructing phylogenetic trees [J]. Molecular Biology and Evolution,1987,4(4):406-425.
[31]TAMURA K, DUDLEY J, NEI M, et al. MEGA4:Molecular evolutionary genetics analysis (MEGA) software version 4.0 [J]. Molecular Biology and Evolution,2007,24(8):1596-1599.
[32]SAKURAI J, ISHIKAWA F, YAMAGUCHI T, et al. Identification of 33 rice aquaporin genes and analysis of their expression and function [J]. Plant and Cell Physiology,2005,46(9):1568-1577.
[33]夏雯雯,李锦,祝建波. 天山雪莲水孔蛋白基因(AQP)家族鉴定与生物信息学分析[J]. 江苏农业学报,2016,32(6):1244-1250.
[34]孔维龙,胡瑞,包满珠,等. 香石竹水孔蛋白的克隆及表达分析[J]. 园艺学报,2017,44(3):515-527.
[35]NIITTYL T, FUGLSANG A T, PALMGREN M G, et al. Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of arabidopsis [J]. Molecular & Cellular Proteomics,2007,6(10):1711-1726.
[36]SCHFFNER A R. Aquaporin function, structure, and expression:are there more surprises to surface in water relations? [J]. Planta,1998,204:131-139.
[37]SUGA S, MURAI M, KUWAGATA T, et al. Differences in aquaporin levels among cell types of radish and measurement of osmotic water permeability of individual protoplasts [J]. Plant Cell Physiology,2003,44(3):277-286.
[38]PRADO K, MAUREL C. Regulation of leaf hydraulics:From molecular to whole plant levels [J]. Frontiers in Plant Science,2013,4:255.
[39]HACHEZ C, VESELOV D, YE Q, et al. Short-term control of maize cell and root water permeability through plasma membrane aquaporin isoforms [J]. Plant Cell & Environment,2012,35(1):185-198.
[40]ALEXANDERSSON E, FRAYSSE L, SJVALL-LARSEN S, et al. Whole gene family expression and drought stress regulation of aquaporins [J]. Plant Molecular Biology,2005,59(3):469-484.
[41]TYERMAN S, BOHNERT H J, MAUREL C, et al. Plant aquaporins:Their molecular biology, biophysics and significance for plant water relations [J]. Journal of Experimental Botany,1999,50(SI):1055-1071.
[42]MAUREL C, JAVOT H,LAUVERGEAT V, et al. Molecular physiology of aquaporins in plants [J]. International Review of Cytology,2002,215:105-148.
[43]LUU D, MAUREL C. Aquaporins in a challenging environment:molecular gears for adjusting plant water status [J]. Plant Cell & Environment,2005,28(1):85-96.
[44]FORREST K L, BHAVE M. Major intrinsic proteins (MIPs) in plants:a complex gene family with major impacts on plant phenotype [J]. Functional & Integrative Genomics,2007,7(4):263-289.
[45]BESSE M, KNIPFER T, MILLER A J, et al. Developmental pattern of aquaporin expression in barley (Hordeum vulgare L.) leaves [J]. Journal of Experimental Botany,2011,62(12):4127-4142.
[46]SAKURAI J, AHAMED A, MURAI M, et al. Tissue and cell-specific localization of rice aquaporins and their water transport activities [J]. Plant and Cell Physiology,2008,49(1):30-39.
[47]HEINEN R B, YE Q, CHAUMONT F. Role of aquaporins in leaf physiology [J]. Journal of Experimental Botany,2009,60(11):2971-2985.
[48]丁岳炼, 林燕飞,何生根,等. 半定量RT-PCR法分析水孔蛋白基因EgPIP1;3在洋桔梗切花中的表达[J]. 仲恺农业工程学院学报,2012,25(1):6-10.
[49]陈虎,何新华,罗聪,等. 龙眼水通道蛋白基因(DLPIP1)的克隆与表达分析[J]. 果树学报,2012,29(2):225-230.
[50]RODRíGUEZ-GAMIR J, XUE J, CLEARWATER M J, et al. Aquaporin regulation in roots controls plant hydraulic conductance, stomatal conductance, and leaf water potential in Pinus radiata under water stress [J]. Plant Cell & Environment,2019,42(2):717-729.
[51]VANDELEUR R K, MAYO G, SHELDEN M C, et al. The role of plasma membrane intrinsic protein aquaporins in water transport through roots:diurnal and drought stress responses reveal different strategies between isohydric and anisohydric cultivars of grapevine [J]. Plant Physiology,2009,149(1):445-460.
[52]SHATIL-COHEN A, ATTIA Z, MOSHELION M. Bundle-sheath cell regulation of xylem-mesophyll water transport via aquaporins under drought stress:a target of xylem-borne ABA? [J]. Plant Journal,2011,67:72-80.
[53]SUN X B, DENG Y M, LIANG L J, et al. Overexpression of a PIP1 gene from salicornia bigelovii in tobacco plants improves their drought tolerance [J]. Journal of the American Society for Horticultural Science,2017,142(4):235-245.
[54]SHAREENA S, UPENDRA K S S, THUMBALLI R. Constitutive and stress-inducible overexpression of a native aquaporin gene (MusaPIP2;6) in transgenic banana plants signals its pivotal role in salt tolerance [J]. Plant Molecular Biology,2015,88(1/2):41-52.
[55]LIN R, ZHENG J, PU L, et al. Genome-wide identification and expression analysis of aquaporin family in Canavalia rosea and their roles in the adaptation to saline-alkaline soils and drought stress [J]. BMC Plant Biology,2021,21(1):333.
[56]AHARON R, SHAHAK Y, WININGER S, et al. Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress [J]. Plant Cell,2003,15:439-447.
[57]GRONDIN A, MAULEON R, VADEZ V, et al. Root aquaporins contribute to whole plant water fluxes under drought stress in rice (Oryza sativa L.)[J]. Plant Cell & Environment,2016,39(2):347-365.
[58]NORTH G B, MARTRE P, NOBEL P S. Aquaporins account for variations in hydraulic conductance for metabolically active root regions of Agave deserti in wet, dry, and rewetted soil [J]. Plant Cell & Environment,2004,27(2):219-228.
[59]赵勤. 木本蔬菜香椿芽贮藏保鲜研究进展[J]. 贵州林业科技,2008,36(3):55-58.
[60]王清,刘涛. 香椿贮藏保鲜及加工应用的研究进展[J]. 农产品加工,2015(390):55-58,60.

备注/Memo

备注/Memo:
收稿日期:2022-11-27基金项目:安徽省高等学校自然科学基金重点项目(KJ2021A0683);阜阳师范大学-阜阳市2021年度市校合作科技专项(SXHZ202107);2020年度高校优秀拔尖人才培育资助项目(gxgwfx2020049);阜阳师范大学自然科学重点项目(2020FSKJ01ZD)作者简介:赵胡(1977-),男,安徽六安人,博士,副教授,主要从事植物分子生物学研究。(E-mail)zhaohu8196@sina.com通讯作者:石潇瀑,(E-mail)tiramisu.shi@163.com
更新日期/Last Update: 2024-01-15