[1]戚良轩,徐晴玉,李晶,等.灰飞虱唾液鞘形态及其蛋白质组分鉴定[J].江苏农业学报,2023,(01):30-36.[doi:doi:10.3969/j.issn.1000-4440.2023.01.004]
　QI Liang-xuan,XU Qing-yu,LI Jing,et al.Morphology and protein identification of salivary sheath from Laodelphax striatellus[J].,2023,(01):30-36.[doi:doi:10.3969/j.issn.1000-4440.2023.01.004]
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灰飞虱唾液鞘形态及其蛋白质组分鉴定()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年01期
页码:
30-36
栏目:
植物保护
出版日期:
2023-02-28

文章信息/Info

Title:
Morphology and protein identification of salivary sheath from Laodelphax striatellus
作者:
戚良轩1; 徐晴玉1; 李晶1; 鞠佳菲1; 孙洋2; 方继朝1; 3; 纪锐1; 2; 3
（1.江苏省农业科学院植物保护研究所，江苏南京210014；2.安徽师范大学生命科学学院/安徽省重要生物资源保护与利用研究重点实验室，安徽芜湖241000；3.淮阴师范学院/江苏省区域现代农业与环境保护协同创新中心,江苏淮安223300）
Author(s):
QI Liang-xuan1; XU Qing-yu1; LI Jing1; JU Jia-fei1; SUN Yang2; FANG Ji-chao1; 3; JI Rui1; 2; 3
（1.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.Key Laboratory for Conservation and Use of Important Biological Resources of Anhui Province/College of Life Sciences, Anhui Normal University, Wuhu 241000, China;3.Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection/Huaiyin Normal University, Huai′an 223300, China）
关键词:
灰飞虱; 唾液鞘; 蛋白质组分
Keywords:
Laodelphax striatellus; salivary sheath; protein component
分类号:
S435.112
DOI:
doi:10.3969/j.issn.1000-4440.2023.01.004
文献标志码:
A
摘要:
灰飞虱是一种小型的刺吸式口器昆虫，是危害水稻的主要害虫之一。灰飞虱刺吸取食时分泌的胶状唾液可形成唾液鞘，保护口针并帮助取食，同时其中的蛋白质效应子在调控作物免疫中扮演重要角色。本研究利用扫描电子显微镜观察了灰飞虱唾液鞘的形态：多呈树枝状，表面为较为光滑的珠状结构。应用双层膜装置收集了若虫的唾液鞘，经液相色谱与串联质谱联用检测鉴定得到42种灰飞虱唾液鞘蛋白质，其中19种蛋白质检测到2个及以上的唯一肽段。进一步分析了编码这19种蛋白质的基因在各个组织中的表达模式，发现其中8个基因在唾液腺中明显高表达，推测其可能参与了唾液鞘的形成以及害虫-作物的互作。唾液鞘蛋白质组分鉴定为后续筛选和研究灰飞虱效应子功能提供了基础，有助于明确灰飞虱-水稻互作的分子机制，为开发害虫绿色防控新策略提供思路。
Abstract:
Small brown planthopper (SBPH) Laodelphax striatellus, the tinny sap-sucking insects, is one of the most destructive herbivores damaging rice in China. During the feeding process, gel saliva secreted by SBPHs can form the salivary sheath to protect stylet and help feeding. Some protein effectors in the salivary sheath play important roles in regulating plant immunity. Here, we observed the morphology of SBPH salivary sheaths under scanning electron microscope: most salivary sheaths were dendritic and had smooth bead structures in the surface. The salivary sheaths of SBPH nymphs were collected using double membrane device. After the liquid chromatography-tandem mass spectrometer (LC-MS/MS)analysis, 42 proteins were finally identified in the salivary sheaths, and among them, 19 proteins with two or more unique peptides were detected. Using quantitative polymerase chain reaction, we analyzed the expression patterns of genes encoding these 19 proteins in different tissues of SBPH, and found eight genes with dramatically higher expression levels in the salivary gland, suggesting their importance in the formation of salivary sheaths and interaction of insect and plant. These results lay a solid foundation for studying the function of SBPH salivary sheath, revealing the detailed mechanism of SBPH-rice interaction, and developing green pest management strategies.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2022-09-26基金项目：国家自然科学基金面上项目（31871965）；“十四五”国家重点研发计划项目（ 2021YFD1401100）；江苏省农业科技自主创新基金项目［CX（22）3018］作者简介：戚良轩（1993-），男，安徽铜陵人，博士，助理研究员，主要从事作物与害虫互作研究，（E-mail）470724564@qq.com。徐晴玉为共同第一作者。通讯作者：纪锐，（E-mail）jirui@jaas.ac.cn；方继朝，（E-mail）fangjc@jaas.ac.cn
更新日期/Last Update: 2023-03-21