[1]刘晴晴,李勇,张明霞,等.紫色桑葚和白色桑葚总酚含量、抗氧化能力及代谢指纹图谱差异分析[J].江苏农业学报,2022,38(03):813-820.[doi:doi:10.3969/j.issn.1000-4440.2022.03.029]
　LIU Qing-qing,LI yong,ZHANG Ming-xia,et al.Differences in total phenol content, antioxidant activity and metabolic fingerprint between purple mulberry and white mulberry[J].,2022,38(03):813-820.[doi:doi:10.3969/j.issn.1000-4440.2022.03.029]
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紫色桑葚和白色桑葚总酚含量、抗氧化能力及代谢指纹图谱差异分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年03期
页码:
813-820
栏目:
加工贮藏·质量安全
出版日期:
2022-06-30

文章信息/Info

Title:
Differences in total phenol content, antioxidant activity and metabolic fingerprint between purple mulberry and white mulberry
作者:
刘晴晴1; 2; 3; 李勇2; 3; 张明霞2; 余向阳2; 杨晨晔4; 武国华1
（1.江苏科技大学生物技术学院，江苏镇江212000；2.省部共建国家重点实验室培育基地/江苏省食品质量安全重点实验室，江苏南京210014；3.江苏大学食品与生物工程学院，江苏镇江212000；4.江苏省农业科学院中心实验室，江苏南京210014）
Author(s):
LIU Qing-qing1; 2; 3; LI yong2; 3; ZHANG Ming-xia2; YU Xiang-yang2; YANG Chen-ye4; WU Guo-hua1
（1.School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212000， China;2.Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology， Nanjing 210014， China;3.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212000， China；4.Central Laboratory, Jiangsu Academy of Agricultural Sciences， Nanjing 210014， China ）
关键词:
桑葚; 代谢组学; 总酚含量; 抗氧化能力; 代谢通路
Keywords:
mulberry; metabolomics; total phenol content; antioxidant activity; metabolic pathway
分类号:
S663.9
DOI:
doi:10.3969/j.issn.1000-4440.2022.03.029
文献标志码:
A
摘要:
为深入了解桑葚的营养价值，本研究重点比较了紫色桑葚和白色桑葚样品中总酚含量、抗氧化能力以及代谢图谱差异。结果表明，2种颜色的桑葚总酚含量和抗氧化能力存在明显差异，紫色桑葚抗氧化能力明显高于白色。基于LC-QTOF/MS的代谢组学分析结果表明，2种颜色的桑葚代谢图谱存在明显差异，共筛选出136种差异代谢物，包括类黄酮、花色苷、酚酸、氨基酸、核苷类、有机酸、糖类、脂类等。紫色桑葚样品中类黄酮、花青素物质含量比白色桑葚多。白色桑葚中常见酚酸含量（阿魏酸、二咖啡酰奎宁酸、香豆酸和绿原酸）明显高于紫色桑葚。紫色桑葚中糖醇和糖磷酸含量较高，而白色桑葚中二糖和三糖物质含量较高；紫色桑葚中葡萄糖酸、哌啶酸、黏液酸、琥珀酸等有机酸含量较高，而白色桑葚中奎尼酸、柠檬酸、苹果酸、马来酸含量较高，说明2种颜色的桑葚糖酸风味来源可能不同。紫色桑葚中亮氨酸、缬氨酸、异亮氨酸含量较高，而白色桑葚中与次级代谢物的合成前体相关的苯丙氨酸、色氨酸、络氨酸含量较高。此外，紫色桑葚中核苷类物质含量较高，而白色桑葚中生物碱及脂类物质含量较多。基于差异代谢物的代谢通路分析发现，紫色桑葚和白色桑葚差异代谢通路主要包括类黄酮代谢、亚油酸代谢、异奎宁生物碱合成、苯丙氨酸代谢等。
Abstract:
To further understand the nutritional value of mulberry, the total phenol content, antioxidant activity and metabolic fingerprint in purple and white mulberries were compared. The results showed that there were significant differences in total phenol content and antioxidant capacity between the two colors of mulberry, and the antioxidant capacity of purple mulberry was significantly higher than that of white mulberry. The results of metabolomics analysis based on LC-QTOF/MS revealed that there were significant differences in metabolic profiles between purple and white mulberries. A total of 136 metabolites with significant difference were selected, including flavonoids, anthocyanins, phenolic acids, amino acids, nucleosides, organic acids, sugars, lipids, and so on. The contents of flavonoids and anthocyanins in purple mulberries were higher than those in white mulberries. The contents of common phenolic acids (ferulic acid, dicaffeoylquinic acid, coumaric acid and chlorogenic acid) in white mulberry were significantly higher than those in purple mulberry. The contents of sugar alcohol and sugar phosphoric acid in purple mulberry were higher, while the contents of disaccharide and trisaccharide in white mulberry were higher. The contents of organic acids such as gluconic acid, piperidic acid, muconic acid and succinic acid in purple mulberry were higher, while the contents of quinic acid, citric acid, malic acid and maleic acid in white mulberry were higher, indicating that the sugar and acid flavor sources in purple and while mulberries may be different. The contents of leucine, valine and isoleucine in purple mulberry were higher, while the contents of phenylalanine, tryptophan and tyrosine related to the synthetic precursors of secondary metabolites were higher in white mulberry. In addition, the content of nucleosides in purple mulberry was higher, while the contents of alkaloids and lipids in white mulberry were higher. Based on the analysis of metabolic pathways of differential metabolites, the important pathways included the flavonoid metabolism, linoleic acid metabolism, isoquinine alkaloid synthesis, phenylalanine metabolism, etc.

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备注/Memo

备注/Memo:
收稿日期：2021-09-16基金项目：江苏省农业科技自主创新基金项目[CX(19)3006]作者简介：刘晴晴(1997-)，女，安徽阜阳人，硕士研究生，研究方向为畜牧食源性植物代谢组学分析。（E-mail）2862406513@qq.com 通讯作者：李勇，（E-mail）liyong_213@163.com；武国华，（E-mail）ghwu@just.edu.cn
更新日期/Last Update: 2022-07-07