[1]苏秀丽,梁惠凌,刘宝玉,等.基于ISSR分子标记的黄花倒水莲遗传多样性分析[J].江苏农业学报,2022,38(03):605-610.[doi:doi:10.3969/j.issn.1000-4440.2022.03.004]
　SU Xiu-li,LIANG Hui-ling,LIU Bao-yu,et al.Genetic diversity analysis of Polygala fallax based on ISSR molecular markers[J].,2022,38(03):605-610.[doi:doi:10.3969/j.issn.1000-4440.2022.03.004]
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基于ISSR分子标记的黄花倒水莲遗传多样性分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年03期
页码:
605-610
栏目:
遗传育种·生理生化
出版日期:
2022-06-30

文章信息/Info

Title:
Genetic diversity analysis of Polygala fallax based on ISSR molecular markers
作者:
苏秀丽1; 2; 3; 梁惠凌1; 2; 刘宝玉1; 2; 黄夕洋1; 2; 唐辉1; 2
（1.广西壮族自治区中国科学院广西植物研究所，广西桂林541006；2.广西植物功能物质研究与利用重点实验室，广西桂林541006；3.广西师范大学珍稀濒危动植物生态与环境保护教育部重点实验室，广西桂林541006）
Author(s):
SU Xiu-li1; 2; 3; LIANG Hui-ling1; 2; LIU Bao-yu1; 2; HUANG Xi-yang1; 2; TANG Hui1; 2
(1.Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, China;2.Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guilin 541006, China；3.Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin 541006, China)
关键词:
黄花倒水莲; 简单重复序列间扩增（ISSR）分子标记; 遗传多样性分析; 遗传分化
Keywords:
Polygala fallax Hemsl; inter-simple sequence repeat (ISSR) molecular markers; genetic diversity; genetic differentiation
分类号:
S567.23+9
DOI:
doi:10.3969/j.issn.1000-4440.2022.03.004
文献标志码:
A
摘要:
旨在揭示黄花倒水莲资源的遗传多样性，分析其遗传差异，为黄花倒水莲的良种选育提供理论基础。以荷包山桂花居群为外类群，利用简单重复序列间扩增（Inter simple sequence repeat, ISSR）分子标记对来源于10个地区的黄花倒水莲自然居群进行遗传多样性和亲缘关系分析。结果显示，用11条引物共扩增得到132条条带，其中91条为多态性条带（PPB），多态性条带比例为68.94%。在物种水平上，黄花倒水莲10个居群的Nei’s基因多样性指数（H）、Shannon多样性指数（I）分别为0.163 7、0.250 1，表现出较高的遗传多样性，而在居群水平上，H、 I的均值分别为0.063 4、0.098 8，表现出偏低的遗传多样性。Nei’s遗传多样性和分子方差分析（AMOVA）结果表明，黄花倒水莲的遗传变异主要发生在居群间，遗传分化程度较高（基因分化系数=0.610 9，基因流=0.318 5）。遗传一致度及聚类分析结果显示，黄花倒水莲各居群间的亲缘关系较近（遗传距离为0.032～0.182），与荷包山桂花居群的亲缘关系较远（遗传距离为0.428～0.536），在遗传一致度为0.630处可以将黄花倒水莲与荷包山桂花区分开，在遗传一致度为0.867处可以将10个黄花倒水莲居群分为3类。由于黄花倒水莲居群水平上的遗传多样性水平较低，今后应对黄花倒水莲野生资源进行合理利用与保护。
Abstract:
The aim of this study is to reveal the genetic diversity of Polygala fallax Hemsl germplasm resources, and analyze their genetic differences, so as to provide theoretical basis for the selection and breeding of Polygala fallax Hemsl germplasm resources. Taking Polygala arillata Buch as the outer group, the inter-simple sequence repeat (ISSR) molecular markers were used to explore the genetic diversity and genetic relationship of natural populations of Polygala fallax Hemsl from ten regions. The results showed that a total of 132 bands were amplified with 11 primers, including 91 polymorphic bands (PPB), and the proportion of PPB was 68.94%. At the species level, Nei’s gene diversity index (H) and Shannon’s diversity index (I) were 0.163 7 and 0.250 1, respectively, showing high genetic diversity. At the population level, the mean values of H and I were 0.063 4 and 0.098 8, respectively, showing low genetic diversity. The results of Nei’s genetic diversity and molecular variance analysis (AMOVA) showed that the genetic variation was mainly distributed among populations, with a large level of genetic differentiation (gene differentiation coefficient was0.610 9, gene flow was 0.318 5). The genetic identity and clustering results indicated that the genetic relationship between the populations of Polygala fallax Hemsl was relatively close (genetic distance was 0.032-0.182), and the genetic relationship with Polygala arillata Buch was relatively far (genetic distance was 0.428-0.536). At the level of genetic identity of 0.630, Polygala fallax Hemsl and Polygala arillata Buch (YN) could be separated. Ten populations of Polygala fallax Hemsl could be divided into three types at 0.867. In view of the relatively low level of genetic diversity in the population of Polygala fallax Hemsl, the wild resources should be rationally utilized and protected in the future.

参考文献/References:

[1]王子威,何中声,刘金福. 黄花倒水莲栽培及利用研究综述[J]. 中国野生植物资源, 2016, 35(4): 48-52.
[2]陈秀香,梁定仁,黄宝山,等. 广西靖西县传统药市壮药调查初报[J]. 中国中药杂志, 1992(1): 6-7,62.
[3]戴斌,丘翠嫦,周丽娜,等. 瑶药“结端旁”（黄花参）的生药学研究[J]. 中国民族医药杂志, 1996(2): 33-34.
[4]曹后康,韦日明,张可锋,等. 黄花倒水莲多糖对四氯化碳致急性肝损伤小鼠的保护作用[J]. 中药材, 2018, 41(1): 203-206.
[5]张嫦丽,张可锋,许有瑞,等. 黄花倒水莲的化学成分与药理活性研究进展[J]. 中国药房, 2017, 28(19): 2724-2728.
[6]陈家宝,潘为高,罗彭,等. 黄花倒水莲的研究进展[J]. 亚太传统医药, 2018, 14(5): 86-89.
[7]许立拔,龙莉,谢凤凤,等. 黄花倒水莲药理研究进展[J]. 壮瑶药研究, 2019(1): 77-82,106.
[8]李佳,房敏峰,周天华,等. 主产区远志种质资源遗传多样性的ISSR分析[J]. 中草药, 2010, 41(11): 1881-1885.
[9]刘超,秦金山,韩文兰,等. 山西省西伯利亚远志种质资源的ISSR分析[J]. 热带农业科学, 2015, 35(4): 45-50.
[10]杨栋林,陈治秀,罗慧蓉,等. 广西10个地理居群海菜花遗传多样性的ISSR分析[J]. 分子植物育种, 2021：1-19. https://kns-cnki-net.webvpn.gxnu.edu.cn/kcms/detail/46.1068.s.20210223.1030.008.html.
[11]张建波,鄢家俊,白史且,等. 基于ISSR标记的野生斑茅居群遗传结构研究[J]. 中国草地学报, 2017, 39(3): 23-30.
[12]覃信梅,蒋水元,韩愈,等. 不同特异种质罗汉果的遗传差异及杂交优势预测[J]. 江苏农业学报, 2018, 34(2): 425-431.
[13]陈宗游,黄夕洋,唐辉,等. 广西甜茶种质资源遗传多样性的ISSR分析[J]. 园艺学报, 2017, 44(1): 161-169.
[14]张向前,李予霞,祝建波,等. 古尔班通古特沙漠荒漠肉苁蓉遗传多样性分析[J]. 基因组学与应用生物学, 2019, 38(8): 3675-3680.
[15]王祎玲,赵桂仿. 七筋菇自然居群的遗传结构分析[J]. 云南植物研究, 2007，29(3): 293-299.
[16]王晓彤,罗点,陈高,等. 对叶百部遗传多样性的ISSR分析[J]. 中草药, 2017, 48(19): 4051-4056.
[17]张盾,任梦云,张银东,等. 基于ISSR分子标记的野生山莨菪遗传多样性研究[J]. 中草药, 2018, 49(1): 219-226.
[18]NYBOM H. Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants[J]. Molecular Ecology, 2004, 13(5): 1143-1155.
[19]和志娇,和加卫,程在全,等. 三叶悬钩子自然居群遗传多样性的ISSR分析[J]. 园艺学报, 2012, 39(11): 2142-2150.
[20]COSTANTINO R F. The genetical structure of populations and developmental time[J]. Genetics, 1968，60(2): 409-418.
[21]WRIGHT S. The genetical structure of populations[J]. Annals of Eugenics,1951, 15(4): 323-354.
[22]李斌,费希同,唐军荣,等. ‘黄花倒水莲’离体快繁技术研究[J]. 甘肃农业大学学报, 2016, 51(4): 37-42.
[23]史艳财,邹蓉,韦记青,等. 黄花倒水莲种子萌发特性研究[J]. 北方园艺, 2013(19): 159-161.
[24]VOLIS S. Securing a future for China’s plant biodiversity through an integrated conservation approach[J]. Plant Diversity, 2018, 40(3): 91-105.

备注/Memo

备注/Memo:
收稿日期：2021-10-13基金项目：广西科技重大专项（桂科AA18118015）；桂林市重大专项(20190101)；桂林市科学研究与技术开发计划项目（20190208-3）；广西植物功能物质研究与利用重点实验室项目（ZRJJ2018-7、ZRJJ2020-5）作者简介：苏秀丽（1996-），女，广西贵港人，硕士研究生，研究方向为生物化学与分子生物学。(E-mail)1808891670@qq.com通讯作者：黄夕洋，(E-mail)57643787@qq.com；唐辉，(E-mail)913529761@qq.com
更新日期/Last Update: 2022-07-07