[1]吴剑梅,傅丹丹,李佳璇,等.ETT2转录因子EivF调控禽致病性大肠杆菌运动性和环境胁迫耐受性[J].江苏农业学报,2022,38(02):438-445.[doi:doi:10.3969/j.issn.1000-4440.2022.02.018]
　WU Jian-mei,FU Dan-dan,LI Jia-xuan,et al.Regulation of ETT2 transcription factor EivF on avian pathogenic Escherichia coli motility and tolerance to environmental stress[J].,2022,38(02):438-445.[doi:doi:10.3969/j.issn.1000-4440.2022.02.018]
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ETT2转录因子EivF调控禽致病性大肠杆菌运动性和环境胁迫耐受性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年02期
页码:
438-445
栏目:
畜牧兽医·水产养殖
出版日期:
2022-04-30

文章信息/Info

Title:
Regulation of ETT2 transcription factor EivF on avian pathogenic Escherichia coli motility and tolerance to environmental stress
作者:
吴剑梅; 傅丹丹; 李佳璇; 邵颖; 宋祥军; 涂健; 祁克宗
（安徽农业大学兽医病理生物学与疫病防控安徽省重点实验室，安徽省动物性食品质量与生物安全工程实验室，安徽合肥230036）
Author(s):
WU Jian-mei;  FU Dan-dan;  LI Jia-xuan;  SHAO Ying;  SONG Xiang-jun;  TU Jian;  QI Ke-zong
(Anhui Province Key Laboratory of Veterinary Pathology and Disease Control, Anhui Provincial Animal Food Quality and Biosafety Engineering Laboratory, Anhui Agricultural University, Hefei 230036, China)
关键词:
禽致病性大肠杆菌; ETT2转录因子EivF; 鞭毛; 环境胁迫
Keywords:
avian pathogenic Escherichia coli; ETT2 transcription factor EivF; flagella; environmental stress
分类号:
S858.351+.2
DOI:
doi:10.3969/j.issn.1000-4440.2022.02.018
文献标志码:
A
摘要:
为探究禽致病性大肠杆菌（Avian pathogenic Escherichia coli, APEC）中大肠杆菌Ⅲ型分泌系统（Escherichia coli Type Ⅲ secretion system2, ETT2）转录因子EivF的功能，通过透射电镜观察检测野生株(AE81)、缺失株(AE81△eivF)和回复株(AE81△eivF-comp) Curli菌毛的合成能力，并探究3种菌株在不良环境中的存活情况，通过RT-qPCR检测鞭毛和环境耐受相关基因的转录水平。结果表明，与野生株相比，缺失株的curli菌毛合成能力无明显变化，在透射电镜下缺失株鞭毛数量明显减少，缺失株在强酸、强碱、氧化应激、高温、高渗环境中的存活率均显著（P<0.05）或极显著（P<0.01）降低，回复株上述表型基本恢复。转录组数据显示，缺失株的flgB、flgC、flgE等鞭毛相关基因和proV、cadA等环境耐受相关基因的转录水平下调。RT-qPCR结果显示，flgB、flgC、flgE、proV等基因的转录水平下调。综上所述，ETT2转录因子EivF参与调控禽致病性大肠杆菌运动能力和在不良环境中的存活能力。
Abstract:
To investigate the function of transcription factor EivF of Escherichia coli Type Ⅲ secretion system2 (ETT2) in avian pathogenic E. coli (APEX), the synthetic ability for Curli pili of wild strain (AE81), deleted strain (AE81△eivF) and revertant strain (AE81△eivF-comp) were detected by transmission electron microscopy, and the survival condition of three strains in adverse environment was investigated. The transcriptional levels of flagella and environmental tolerance related genes were detected by RT-qPCR. The results showed that, there was no significant change in Curli pili synthetic ability of the deleted strain compared with the wild strain. The flagella number of deleted strain (AE81△eivF) decreased significantly under transmission electron microscope. The survival rates of deleted strain under the environments of strong acid, strong alkali, oxidative stress, high temperature and hyperosmotic pressure were significantly（P<0.05）or extremely significantly (P<0.01) decreased, and the above phenotype was basically restored in the revertant strain. Transcriptome data showed that, the transcriptional levels of flagellum-related genes such as flgB, flgC, flgE and environmental tolerance related genes such as proV, cadA were down-regulated in the deleted strains. The results of RT-qPCR showed that, the transcriptional levels of flgB, flgC, flgE, proV and other genes were down-regulated. In conclusion, the ETT2 transcriptional factor EivF is involved in the regulation of motor ability and survival ability of avian pathogenic E. coli in adverse environments.

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备注/Memo

备注/Memo:
收稿日期：2021-06-21基金项目：国家自然科学基金项目(31772707);安徽农业大学2019年度研究生创新基金项目(2019ysj-24)作者简介：吴剑梅(1998-)，女，安徽安庆人，硕士研究生，主要从事动物性食品安全研究。（E-mail）1916679282@qq.com通讯作者：祁克宗，（E-mail）qkz@ahau.edu.cn
更新日期/Last Update: 2022-05-07