[1]何琪,孙晨晨,侯曼曼,等.禽致病性大肠杆菌hcp2a基因对雏鸡脾脏细胞因子-细胞因子受体相互作用通路的影响[J].江苏农业学报,2022,38(01):151-156.[doi:doi:10.3969/j.issn.1000-4440.2022.01.018]
　HE Qi,SUN Chen-chen,HOU Man-man,et al.Effects of avian pathogenic Escherichia coli (APEC) hcp2a gene on the cytokine-cytokine receptor interaction pathway in chick spleen[J].,2022,38(01):151-156.[doi:doi:10.3969/j.issn.1000-4440.2022.01.018]
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禽致病性大肠杆菌hcp2a基因对雏鸡脾脏细胞因子-细胞因子受体相互作用通路的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年01期
页码:
151-156
栏目:
畜牧兽医·水产养殖
出版日期:
2022-02-28

文章信息/Info

Title:
Effects of avian pathogenic Escherichia coli (APEC) hcp2a gene on the cytokine-cytokine receptor interaction pathway in chick spleen
作者:
何琪; 孙晨晨; 侯曼曼; 肖福泉; 龚柳菲; 黄燕; 段少仪; 祁克宗; 宋祥军
（安徽农业大学动物科技学院，兽医病理生物学与疫病防控安徽省重点实验室，安徽合肥230036）
Author(s):
HE Qi; SUN Chen-chen; HOU Man-man; XIAO Fu-quan; GONG Liu-fei; HUANG Yan; DUAN Shao-yi; QI Ke-zong; SONG Xiang-jun
(College of Animal Science and Technoogy, Anhui Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agricultural University, Hefei 230036, China)
关键词:
禽致病性大肠杆菌; hcp2a基因; 脾脏; 雏鸡; 细胞因子-细胞因子受体相互作用通路
Keywords:
avian pathogenic Escherichia coli; hcp2a gene; spleen; chick; cytokine-cytokine receptor interaction pathway
分类号:
S831
DOI:
doi:10.3969/j.issn.1000-4440.2022.01.018
文献标志码:
A
摘要:
探究禽致病性大肠杆菌（APEC）hcp2a基因缺失对雏鸡脾脏转录组的影响，为深入研究禽致病性大肠杆菌的致病机理奠定理论基础。将7日龄雏鸡随机分成2组，用APEC野生株（AE17）及其hcp2a基因缺失株（AE17△hcp2a）分别感染雏鸡，采集脾脏组织制作苏木精-伊红(HE)染色切片，通过转录组学测序筛选hcp2a基因缺失株感染后的差异表达基因，利用实时荧光定量PCR方法对测序结果进行验证，对差异表达基因进行GO、KEGG分析。AE17和AE17△hcp2a均能引起雏鸡脾脏的病理变化，转录组学测序结果发现，AE17△hcp2a感染雏鸡脾脏中筛选到512个差异表达基因，其中221个基因上调表达，291个基因下调表达。选择部分差异表达基因进行实时荧光定量PCR验证，差异表达基因mRNA转录水平的变化趋势与测序结果一致。GO分析结果表明，差异表达基因富集在生物膜、生物膜的组成成分、氧化还原过程、免疫反应、水解酶活性等条目。KEGG分析结果表明，差异表达基因富集在细胞因子-细胞因子受体相互作用通路、细胞粘附分子（CAMs）通路等。禽致病性大肠杆菌hcp2a基因缺失后感染雏鸡，会影响其脾脏mRNA表达谱，差异表达基因主要富集在细胞因子-细胞因子受体相互作用通路等。
Abstract:
In order to explore the role of hcp2a gene in the process of avian pathogenic Escherichia coli (APEC) infection, the spleen transcriptome of chicken infected with hcp2a-delected APEC was analyzed. Seven-day-old chickens were divided into two groups randomly, which were infected with APEC wild strain (AE17) and hcp2a-delected strain (AE17△hcp2a) by intramuscular injection. Then infected spleen tissues were collected for hematoxylin-eosin (HE) sections. Differentially expressed genes infected by AE17△hcp2a were screened by transcriptome sequencing. The sequencing results were verified by real-time fluorescent quantitative PCR, and the differentially expressed genes were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Splenic lesions caused by AE17 and the AE17△hcp2a were observed. The results of transcriptome sequencing indicated that 512 differentially expressed genes were screened from the spleens of chicks infected with AE17△hcp2a, and 221 genes were up-regulated and 291 genes were down-regulated. Some differentially expressed genes were selected for real-time fluorescent quantitative PCR verification, and the variation trend was consistent with the sequencing results. The results of GO analysis showed that the differentially expressed genes were enriched in biofilm and membrane components, oxidation-reduction process, immune response and hydrolase activity. The results of KEGG analysis indicated that the differentially expressed genes were enriched in the cytokine-cytokine receptor interaction pathway and cell adhesion molecules (CAMs) pathway. The mRNA expression profile in spleen of chicken infected with AE17△hcp2a is affected, and the differentially expressed genes are mainly enriched in the cytokine-cytokine receptor interaction pathway.

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备注/Memo

备注/Memo:
收稿日期：2021-05-30基金项目：2020年省级大学生创新创业计划项目(S202010364022)；安徽省重点研究与开发计划项目(202104f06020010)；国家青年科学基金项目(31802161)作者简介：何琪（2000-），女，安徽宣城人，本科，主要从事禽致病性大肠杆菌病的研究。(E-mail)1534558542@qq.com通讯作者：宋祥军，(E-mail)sxj@ahau.edu.cn
更新日期/Last Update: 2022-03-04