[1]原冬伟,王一,栾广宇,等.整合素αvβ1在猪流行性腹泻病毒(PEDV)侵染Vero细胞过程中的作用[J].江苏农业学报,2022,38(02):422-428.[doi:doi:10.3969/j.issn.1000-4440.2022.02.016]
 YUAN Dong-wei,WANG Yi,LUAN Guang-yu,et al.Role of integrin αvβ1 for porcine epidemic diarrhea virus (PEDV) infection in Vero cells[J].,2022,38(02):422-428.[doi:doi:10.3969/j.issn.1000-4440.2022.02.016]
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整合素αvβ1在猪流行性腹泻病毒(PEDV)侵染Vero细胞过程中的作用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年02期
页码:
422-428
栏目:
畜牧兽医·水产养殖
出版日期:
2022-04-30

文章信息/Info

Title:
Role of integrin αvβ1 for porcine epidemic diarrhea virus (PEDV) infection in Vero cells
作者:
原冬伟王一栾广宇张旭
(黑龙江八一农垦大学动物科技学院, 黑龙江大庆163319)
Author(s):
YUAN Dong-weiWANG YiLUAN Guang-yuZHANG Xu
(College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China)
关键词:
整合素αvβ1PEDV侵染Vero细胞
Keywords:
integrin αvβ1porcine epidemic diarrhea virus (PEDV)infectionVero cells
分类号:
S858.282.65+9.6
DOI:
doi:10.3969/j.issn.1000-4440.2022.02.016
文献标志码:
A
摘要:
为明确整合素αvβ1在猪流行性腹泻病毒(PEDV)侵染过程中的作用,通过对整合素αvβ1在Vero细胞表面的过表达和抑制反应,检测PEDV在侵染过程中的病毒载量和蛋白质表达量的变化。结果显示,Vero细胞表面整合素αvβ1的过表达使病毒载量和蛋白质表达量升高,Vero细胞表面整合素αvβ1基因的沉默使病毒载量和蛋白质表达量显著降低。整合素αvβ1可促进PEDV侵染Vero细胞。
Abstract:
In this study, we detected the viral load and protein expression of porcine epidemic diarrhea virus (PEDV) after overexpression and inhibition of integrin αvβ1 on the surface of Vero cells, and then cleared the role of integrin αvβ1 in the PEDV infection process. The results showed that the viral load and protein expression were significantly increased in the Vero cells which overexpressed integrin αvβ1, and the viral load and protein expression were significantly reduced in the Vero cells with silent integrin αvβ1 gene. Integrin αvβ1 promotes PEDV to infect Vero cells.

参考文献/References:

[1]李公美,李茂辉,钱爱东,等. 猪流行性腹泻病毒新型检测方法的研究进展[J]. 中国兽医学报, 2020, 40(3): 650-653,664.
[2]WOOD E N. An apparently new syndrome of porcine epidemic diarrhoea [J]. The Veterinary Record, 1977, 100(12): 243-244.
[3]LEE C. Porcine epidemic diarrhea virus: An emerging and re-emerging epizootic swine virus [J]. Virol J, 2015, 12: 193.
[4]SANDRINE B, MILLET J K, LICITRA B N, et al. Mechanisms of coronavirus cell entry mediated by the viral spike protein [J]. Viruses, 2012, 4(6): 1011-1033.
[5]OH J S, SONG D S, PARK B K. Identification of a putative cellular receptor 150 kDa polypeptide for porcine epidemic diarrhea virus in porcine enterocytes [J]. J Vet Sci, 2003, 4(3): 269-275.
[6]LI B X, GE J W, LI Y J. Porcine aminopeptidase N is a functional receptor for the PEDV coronavirus [J]. Virology, 2007, 365(1): 166-172.
[7]CONG Y, LI X, BAI Y, et al. Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells [J]. Virology, 2015, 478: 1-8.
[8]PARK J E, PARK E S, YU J E, et al. Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus [J]. Virus Res, 2015, 197: 108-115.
[9]李公美,幺乃全,李茂辉,等. 猪氨基肽酶对PEDV感染作用的研究进展[J]. 中国兽医科学, 2020, 50(11): 1421-1427.
[10]SHIRATO K, MAEJIMA M, ISLAM M T, et al. Porcine aminopeptidase N is not a cellular receptor of porcine epidemic diarrhea virus, but promotes its infectivity via aminopeptidase activity [J]. J Gen Virol, 2016, 97(10): 2528-2539.
[11]WHITWORTH K M, ROWLAND R R R, PETROVAN V, et al. Resistance to coronavirus infection in amino peptidase N-deficient pigs [J]. Transgenic Res, 2019, 28(1): 21-32.
[12]LI W, LUO R, HE Q, et al. Aminopeptidase N is not required for porcine epidemic diarrhea virus cell entry [J]. Virus Res, 2017,5(2): 6-13.
[13]LUO L, WANG S, ZHU L, et al. Aminopeptidase N-null neonatal piglets are protected from transmissible gastroenteritis virus but not porcine epidemic diarrhea virus [J]. Sci Rep, 2019, 9(1): 13186.
[14]CHRISTEL S, HERRLER G. Sialic acids as receptor determinants for coronaviruses[J]. Glycoconjugate Journal, 2006, 23(1/2): 51-58.
[15]KUBA K, IMAI Y, OHTO N T, et al. Trilogy of ACE2: A peptidase in the renin-angiotensin system, a SARS receptor, and a partner for amino acid transporters[J]. Pharmacology & Therapeutics, 2010, 128(1): 119-128.
[16]JEFFERS S A, HEMMILA E M, HOLMES K V. Human coronavirus 229E can use CD209L (L-SIGN) to enter cells [J]. Advances in Experimental Medicine & Biology, 2006, 581: 265-269.
[17]MADU I G, CHU V C, LEE H, et al. Heparan sulfate is a selective attachment factor for the avian coronavirus infectious bronchitis virus Beaudette[J].Avian Diseases, 2007, 51(1): 45-51.
[18]张俊磊. 整合素在病毒感染宿主细胞过程中的作用[J]. 第三军医大学学报, 2004, 26(2): 176-178.
[19]高晶. 猪整合素αvβ3真核表达载体构建及介导PEDV感染作用的初步验证[D]. 大庆:黑龙江八一农垦大学, 2016.
[20]LI C, SU M, YIN B, et al. Integrin αvβ3 enhances replication of porcine epidemic diarrhea virus on Vero E6 and porcine intestinal epithelial cells [J]. Vet Microbiol, 2019, 237: 108400.
[21]YUN B L, GUAN X L, LIU Y Z, et al. Integrin αvβ1 modulation affects subtype B avian metapneumovirus fusion protein-mediated cell-cell fusion and virus infection [J]. J Biol Chem, 2016, 291(28): 14815-14825.
[22]SHUAI L, WANG J, ZHAO D, et al. Integrin β1 promotes peripheral entry by rabies virus [J]. J Virol, 2020, 94(2): e01819.
[23]DORNER M, ZUCOL F, ALESSI D, et al. β1 integrin expression increases susceptibility of memory B cells to Epstein-Barr virus infection [J]. J Virol, 2010, 84(13): 6667-6677.
[24]XING L, HUHTALA M, PIETIAINEN V, et al. Structural and functional analysis of integrin alpha2I domain interaction with echovirus 1 [J]. J Biol Chem, 2004, 279(12): 11632-11638.
[25]NEFF S, SA-CARVALHO D, RIEDER E, et al. Foot-and-mouth disease virus virulent for cattle utilizes the integrin alpha(v)beta3 as its receptor [J]. J Virol, 1998, 72(5): 3587-3594.
[26]FAN W, QIAN P, WANG D, et al. Integrin αvβ3 promotes infection by Japanese encephalitis virus [J]. Res Vet Sci, 2017, 111: 67-74.
[27]ASKARI J A, BUCKLEY P A, MOULD A P, et al. Linking integrin conformation to function [J]. J Cell Sci, 2009, 122(2): 165-170.
[28]李方华,侯玲玲,苏晓华,等. RNA干扰的研究进展及应用[J]. 生物技术通讯, 2010, 21(5): 740-745.
[29]王龙涛,葛晨霞,王丹,等. 靶向TGEV S基因的外源性microRNA对TGEV增殖效果的抑制[J]. 中国兽医学报, 2016, 36(2): 196-199.

备注/Memo

备注/Memo:
收稿日期:2021-09-16基金项目:黑龙江省自然科学基金项目(C2018049);黑龙江八一农垦大学引进人才科研启动计划资助项目(XYB2014-11);黑龙江八一农垦大学三横三纵支持计划资助项目(ZRCPY201907)作者简介:原冬伟(1980-),男,吉林吉林人,博士,讲师,主要从事动物传染病机制及诊断方法研究。(E-mail)yuandongwei@163.com
更新日期/Last Update: 2022-05-07