[1]杜婕,宋修超,马艳.中药渣堆肥微生物群落结构及纤维素降解酶基因表达量变化特征[J].江苏农业学报,2022,38(02):352-360.[doi:doi:10.3969/j.issn.1000-4440.2022.02.008]
　DU Jie,SONG Xiu-chao,MA Yan.Change characteristics of microbial community structure and cellulose degrading enzyme gene expression level in Chinese medicine residue compost[J].,2022,38(02):352-360.[doi:doi:10.3969/j.issn.1000-4440.2022.02.008]
点击复制

中药渣堆肥微生物群落结构及纤维素降解酶基因表达量变化特征()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年02期
页码:
352-360
栏目:
耕作栽培·资源环境
出版日期:
2022-04-30

文章信息/Info

Title:
Change characteristics of microbial community structure and cellulose degrading enzyme gene expression level in Chinese medicine residue compost
作者:
杜婕1; 2; 3; 4; 宋修超1; 2; 3; 马艳1; 2; 3
（1.江苏省农业科学院农业资源与环境研究所，江苏南京210014；2.农业农村部长江下游农业环境重点实验室，江苏南京210014；3.江苏大学环境与安全工程学院，江苏镇江212013；4.江苏农林职业技术学院畜牧兽医学院，江苏镇江212400）
Author(s):
DU Jie1; 2; 3; 4; SONG Xiu-chao1; 2; 3; MA Yan1; 2; 3
(1.Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China;3.School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China;4.Institute of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, China)
关键词:
中药渣堆肥; Miseq高通量测序; 群落结构; 纤维素降解酶基因
Keywords:
Chinese medicine residue compost; Miseq high-throughput sequencing; community structure; cellulose degrading enzyme genes
分类号:
S141.4
DOI:
doi:10.3969/j.issn.1000-4440.2022.02.008
文献标志码:
A
摘要:
本研究对不同堆制时期的中药渣堆肥样品进行Miseq高通量测序分析，并对8种纤维素降解酶基因进行荧光定量RT-PCR分析，以解析中药渣堆肥过程中微生物群落结构组成及纤维素降解酶基因表达丰度的变化特征。结果表明，在中药渣堆肥过程中，不同堆制时期的微生物群落结构组成不同，并且不同处理间的群落结构也存在差异。对样品中微生物群落进行主成分分析(PCoA)，发现在中药渣堆肥过程中，不同处理的组间微生物群落结构存在较大差异，组内群落结构不断变化并逐渐趋于稳定。荧光定量RT-PCR检测结果显示，在中药渣堆肥过程中，纤维素降解酶基因的表达丰度也处于明显的变化中。说明，中药渣堆肥中微生物群落与纤维素降解酶是相互联系、相互影响的。
Abstract:
In this study, Miseq high-throughput sequencing analysis was performed on samples of Chinese medicine residue compost during different composting periods, and eight kinds of cellulose degrading enzyme genes were analyzed by fluorescence quantitative RT-PCR to explore the composition of microbial community structure and the expression and abundance of cellulose degrading enzyme genes during the composting of Chinese medicine residues. Results showed that during the composting of Chinese medicine residues, the composition of microbial community structure in different periods was different, and the community structure was also different in different treatments. Principal component analysis (PCoA) results indicated that during the composting of Chinese medicine residues, there were significant differences in the microbial community structure among different treatments, and the community structure within the group changed continuously and gradually stabilized. Results of fluorescence quantitative RT-PCR showed that the expression abundance of cellulose degrading enzyme genes also changed significantly during the composting of Chinese medicine residues. In conclusion, the microbial community and cellulose degrading enzymes in Chinese medicine residue compost are interrelated and interacted.

参考文献/References:

[1]杨冰，丁斐，李伟东，等.中药渣综合利用研究进展及生态化综合利用模式[J]. 中草药，2017，48(2)：377-383.
[2]贺灵芝,彭栋梁,梁忠厚. 中药渣开发利用探讨[J]. 科技创新与生产力, 2021(333)：42-44.
[3]周曾艳,余高,陈芬，等. 添加畜禽粪便对中药渣好氧堆肥发酵特性及纤维素、木质素降解率的影响[J]. 河南农业科学, 2020，49(10)：63-69.
[4]袁琪,李伟东,郑艳萍,等．中药渣的深加工及其资源化利用[J]. 生物加工过程，2019，17(2)：171-176.
[5]王春芳,马诗淳,黄艳,等. 降解水稻秸秆的复合菌系及其微生物群落结构演替[J]. 微生物学报，2016, 56(12)： 1856-1868.
[6]李璐. 高效降解中药渣菌群的筛选[D]. 杨凌：西北农林科技大学, 2019.
[7]毛莲英，李海碧，桂意云，等. 基于高通量测序分析间作猫豆对甘蔗根际土壤微生物的影响[J].南方农业学报，2021，52（2）：332-340.
[8]孙瑞萍，王峰，晁哲，等. 1月龄五指山猪与长白猪骨骼肌miRNA转录组比较[J].江苏农业学报，2020,36(3):620-625.
[9]关健飞，曹阳．高通量测序分析黑土稀有微生物群落结构[J]．江苏农业科学，2020,48(20):288-292.
[10]劳承英，韦本辉，周灵芝，等. 袋料种植淮山药根际土壤细菌多样性的高通量测序分析[J].南方农业学报，2020，51（10）：2367-2373.
[11]陈娜,杨毅,张澜,等. Illumina 高通量测序技术分析早产儿出生后肠道菌群变化的初步研究[J]. 中国循证儿科杂志， 2014，9(5)：359-364.
[12]BELLA J M D, BAO Y, GLOOR G B, et al. High throughput sequencing methods and analysis for microbiome research[J]. Journal of Microbiological Methods，2013，95(3)：401-414.
[13]范海青,王凌文,王丹,等. 基于高通量测序的人工湿地微生物群落分析[J]. 科技通报，2019，35(2)：213-219.
[14]胡丹丹,郭婷婷,李娜,等. 以16S rDNA高通量测序法研究果寡糖对奶牛瘤胃菌群结构及多样性的影响[J]. 中国畜牧兽医，2018，45 (10)：2761-2771.
[15]艾士奇,赵一全,孙志远,等. 复合菌系降解纤维素过程中微生物群落结构的变化[J]. 生物工程学报，2018，34(11)：1794-1808.
[16]李淼淼,沈飞,张庆华．纤维素降解复合菌系的微生物多样性及关键功能菌解析[J].环境科学学报，2018，38(7)：2690-2695.
[17]CAPORASO J G, LAUBER C L, WALTERS W A, et al. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample [J].Proceedings of the National Academy of Sciences，2011，108：4516-4522.
[18]MENG T Z, YANG Y J, CAI Z C, et al. The control of Fusarium oxysporum in soil treated with organic material under anaerobic condition is affected by liming and sulfate content [J]. Biology and Fertility of Soils，2018，54：295-307.
[19]CAPORASO J G, KUCZYNSKI J, STOMBAUGH J, et al. QIIME allows analysis of high-throughput community sequencing data [J]. Nat Methods，2010，7：335-336.
[20]SHEN Z Z, RUAN Y Z, WANG B B, et al. Effect of biofertilizer for suppressing Fusarium wilt disease of banana as well as enhancing microbial and chemical properties of soil under greenhouse trial[J]. Applied Soil Ecology，2015，93：111-119.
[21]LIU J F, LIU H Y, QI F S, et al. Application of nano-TiO2/chitosan composite preservative on Penaeus vannamei preservation [J]. Food Science and Technology，2014，39(2)：245-249.
[22]邓辉,王成,吕豪豪,等. 堆肥过程放线菌演替及其木质纤维素降解研究进展[J]. 应用与环境生物学报，2013，19 (4)：581-586.
[23]王志方,陈竞,代金平,等. 棉秸秆自然腐解过程中细菌菌群多样性分析[J]. 新疆农业科学，2019，56(1)：117-125.
[24]侯丽媛,江经纬,蒋建东,等. 假黄单胞菌株J1的筛选及木质纤维素降解基因的生物信息学分析[J]. 南京农业大学学报，2016，39 (4)：573-581.
[25]马宁,魏姜勉,张亮. 一株牛瘤胃耐酸纤维素降解细菌的筛选、鉴定及酶学特性分析[J]. 湖北畜牧兽医，2015，36(6)：7-8.
[26]LYND L R, WEIMER P J, VANZYL W H, et al. Microbial cellulose utilization: fundamentals and biotechnology[J]. Microbiology and Molecular Biology Reviews，2002，66(3)：506-577.
[27]BRETHAUER S, STUDER M H. Biochemical conversion processes of lignocellulosic biomass to fuels and chemicals - a review [J]. Chimia International Journal for Chemistry，2015，69 (10)：572-581.
[28]ZHANG Y H, LYND L R. Toward an aggregated understanding of enzymatic hydrolysis of cellulose: Noncomplexed cellulase systems [J]. Biotechnology and Bioengineering，2004，88 (7)：797-824.
[29]SAWATDEENARUNAT C, SURENDRA K C, TAKARA D, et al. Anaerobic digestion of lignocellulosic biomass: challenges and opportunities [J]. Bioresource Technology，2014，178：178-186.
[30]MOHAMED A H, YOUSEIF S H, EL-MAGEED F H A, et al. Production of cellulase, exoglucanase and xylanase by different microorganisms cultivated on agricultural wastes[J]. Research Journal of Pharmaceutical Biological and Chemical Sciences，2017，8(4)：435-452.
[31]LIM J H, AHN C H, JEONG H Y, et al. Genetic monitoring of multi-functional plant growth promoting rhizobacteria Bacillus subtilis AH18 and Bacillus licheniformis K11 by multiplex and real-time polymerase chain reaction in a pepper farming field[J]. Journal of the Korean Society for Applied Biological Chemistry，2011，54 (2)：221 -228.
[32]BUSSLER L, JACOMINI D, CORRA J M, et al. Recombinant cellulase of Caulobacter crescentus: potential applications for biofuels and textile industries[J].Cellulose，2021，28(5):2813-2832.
[33]MAHMOOD N, AHSAN M, AHMAD I, et al. Molecular expression profile of different cellulolytic enzyme genes in Aspergillus niger in response to UV radiation and chemical mutagenesis [J]. Cellulose Chemistry and Technology, 2014，48(5)：5-6.
[34]UBIPARIP Z, MORENO D S, BEERENS K, et al. Engineering of cellobiose phosphorylase for the defined synthesis of cellotriose [J]. Applied Microbiology and Biotechnology，2020，104 (19)：8327-8337.
[35]HAYASHI T, WONG Y S， MACLACHLAN G. Pea xyloglucan and cellulose: II. hydrolysis by pea endo-1,4-β-glucanases [J]. Plant Physiology，1984，75(3)：605-610.

相似文献/References:

[1]刘蓓一,宦海琳,顾洪如,等.不同发酵时期大麦青贮品质和微生物多样性变化[J].江苏农业学报,2019,(03):653.[doi:doi:10.3969/j.issn.1000-4440.2019.03.021]
　LIU Bei-yi,HUAN Hai-lin,GU Hong-ru,et al.Changes of silage quality and microbial diversity in barley during different fermentation periods[J].,2019,(02):653.[doi:doi:10.3969/j.issn.1000-4440.2019.03.021]

备注/Memo

备注/Memo:
收稿日期：2021-08-27基金项目：国家重点研发计划项目(2017YFD0800200)；江苏省农业科技自主创新基金项目[CX(17)2025]；中国博士后科学基金项目(2018M632255)；江苏农林职业技术学院青年支持项目(2020kj011)作者简介：杜婕（1985-），女，山东泰安人，博士，讲师，主要从事降解功能微生物研究。（E-mail）dujiekycg@163.com通讯作者：马艳，（E-mail）myjaas@sina.com
更新日期/Last Update: 2022-05-07