[1]张斌,杨昕霞,袁志辉.水稻响应热胁迫核心基因的筛选与鉴定[J].江苏农业学报,2021,(04):817-822.[doi:doi:10.3969/j.issn.1000-4440.2021.04.001]
 ZHANG Bin,YANG Xin-xia,YUAN Zhi-hui.Screening and identification of core genes responding to heat stress in rice[J].,2021,(04):817-822.[doi:doi:10.3969/j.issn.1000-4440.2021.04.001]
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水稻响应热胁迫核心基因的筛选与鉴定()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年04期
页码:
817-822
栏目:
遗传育种·生理生化
出版日期:
2021-08-28

文章信息/Info

Title:
Screening and identification of core genes responding to heat stress in rice
作者:
张斌杨昕霞袁志辉
(湖南科技学院,湖南永州425199)
Author(s):
ZHANG BinYANG Xin-xiaYUAN Zhi-hui
(Hunan University of Science and Engineering, Yongzhou 425199, China)
关键词:
水稻高温胁迫核心基因转录组
Keywords:
ricehigh temperature stresscore genetranscriptome
分类号:
S511.01
DOI:
doi:10.3969/j.issn.1000-4440.2021.04.001
文献标志码:
A
摘要:
基于热胁迫条件下的水稻转录组数据,通过HTSeq和DESeq软件筛选差异表达基因;对其进行功能富集和构建蛋白质互作网络,鉴定最重要模块中的核心基因。结果显示:水稻热胁迫0 h、1 h、6 h和12 h条件下,共有278个差异表达基因,生物学过程主要富集在刺激反应和蛋白质折叠上;鉴定出含有12个基因的重要模块.基因表达模式显示重要模块中7个核心基因受热胁迫诱导上调,推测这7个核心基因在水稻响应热胁迫过程中发挥关键作用。
Abstract:
In this study, rice transcriptome data under heat stress were used to screen differentially expressed genes by HTSeq and DESeq software, then functional enrichment analysis was carried out and protein interaction network was constructed to identify the core genes in most important module. The results showed that there were 278 differentially expressed genes under heat stress for 0 h, 1 h, 6 h and 12 h, and the biological process was mainly concentrated in stimulation response and protein folding. The important module containing 12 genes was identified. The gene expression patterns showed that seven core genes in important modules were up-regulated under heat stress, suggesting that these seven core genes played a key role in the response of rice to heat stress.

参考文献/References:

[1]KIM J, SHON J, LEE C K, et al. Relation-ship between grain filling duration and leaf senescence of temperate rice under high temperature[J]. Field Crops Research, 2011, 122: 207-213.
[2]ZHONG L J, CHENG F M, WEN X, et al. The deterioration of eating and cooking quality caused by high temperature during grain filling in early-season indica rice cultivars[J]. Journal of Agronomy & Crop Science, 2005, 191: 218-225.
[3]LIM C J, YANG K A, HONG J K, et al. Gene expression profiles during heat acclimation in Arabidopsis thaliana suspension-culture cells[J]. J Plant Res, 2006, 119: 373-383.
[4]SUZUKI N, SEJIMA H, TAM R, et al. Identification of the MBF1 heat-response regulon of Arabidopsis thaliana[J]. Plant J, 2011, 66(5): 844-851.
[5]GUAN J C, YEH C H, LIN Y P, et al. A 9 bp cis-element in the promoters of class I small heat shock protein genes on chromosome 3 in rice mediates L-azetidine-2-carboxylic acid and heat shock responses[J]. Journal of Experimental Botany, 2010, 61(15): 4249-4261.
[6]JAGADISH S V, MUTHURAJAN R, OANE R, et al. Physiological and proteomic approaches to address heat tolerance during anthesis in rice(Oryza sativa L.)[J]. Journal of Expermental Botany, 2010, 61: 143-156.
[7]JUNG K H, GHO H J, NGUYEN M X, et al. Genome-wide expression analysis of HSP70 family genes in rice and identification of a cytosolic HSP70 gene highly induced under heat stress[J]. Functional & Integrative Genomics, 2013, 13(3): 391-402.
[8]TAKEHARA K, MURATA K, YAMAGUCHI T, et al. Thermo-responsive allele of sucrose synthase 3 (Sus3) provides high-temperature tolerance during the ripening stage in rice (Oryza sativa L.)[J]. Breeding Science, 2018, 68(3): 336-342.
[9]MOON J C, HAM D J, HWANG S G, et al. Molecular characterization of a heat inducible rice gene, OsHSP1, and implications for rice thermotolerance[J]. Genes Genom, 2014, 36: 151-161.
[10]MITTLER R, FINKA A, GOLOUBINOFF P. How do plants feel the heat?[J]. Trends Biochem Sci, 2012, 37(3): 118-125.
[11]陈思婷,郭房庆.植物耐热性及热激信号转导机制研究进展[J].中国科学:生命科学,2013,43(12): 1072-1081.
[12]MORO F, MUGA A. Thermal adaptation of the yeast mitochondrial Hsp70 system is regulated by the reversible unfolding of its nucleotide exchange factor[J]. J Mol Biol,2006, 358(5): 1367-1377.
[13]WANG W, VINOCUR B, SHOSEYOV O, et al. Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response[J]. Trends Plant Sci, 2004, 9(5): 244-252.
[14]HONG S W, VIERLING E. Mutants of Arabidopsis thaliana defective in the acquisition of tolerance to high temperature stress[J]. Proc Natl Acad Sci USA, 2000, 97(8): 4392-4397.
[15]JACKSON C D, KEEGSTRA K. Arabidopsis genes encoding components of the chloroplastic protein import apparatus[J]. Plant Physiol, 2001, 125:1567-1576.
[16]SU P H, LI H M. Stromal Hsp70 is important for protein translocation into pea and Arabidopsis chloroplasts[J].Plant Cell, 2010, 22:1516-1531.
[17]ZHANG X P, GLASER E. Interaction of plant mitochondrial and chloroplast signal peptides with the Hsp70 molecular chaperone[J]. Trends Plant Sci, 2002, 7:14-21.
[18]ZHOU S, SUN H, ZHENG B, et al. Cell cycle transcription factor E2F2 mediates non-stress temperature response of AtHSP70-4 in Arabidopsis[J]. Biochem Biophys Res Commun, 2014, 455(3-4): 139-146.
[19]LEE S, LEE D W, LEE Y, et al. Heat shock protein cognate 70-4 and an E3 ubiquitin ligase, CHIP, mediate plastid-destined precursor degradation through the ubiquitin-26S proteasome system in Arabidopsis[J]. Plant Cell, 2009, 21(12): 3984-4001.
[20]WEI S S, NIU W T, ZHAI X T, et al. Arabidopsis mtHSC70-1 plays important roles in the establishment of COX-dependent respiration and redox homeostasis[J]. J Exp Bot, 2019, 70(20): 5575-5590.
[21]BARRACLOUGH R, ELLIS R J. Protein synthesis in chloroplasts IX assembly of newly-synthesized large subunits into ribulose bisphosphate carboxylase in isolated intact pea chloroplasts[J]. Biochim Biophys Acta, 1980, 608(1):19-31.
[22]HEMMINGSEN S M, ELLIS R J. Purification and properties of ribulosebisphosphate carboxylase large subunit binding protein[J]. Plant Physiology, 1986, 80(1):269-276.
[23]HEMMINGSEN S M, WOOLFORD C, VAN D, et al. Homologous plant and bacterial proteins chaperone oligomeric protein assembly[J]. Nature, 1988, 333(6171):330-334.
[24]ATSUSHI I, HIDEAKI T, MASATO N, et al. Deletion of a chaperonin 60 beta gene leads to cell death in the Arabidopsis lesion initiation 1 mutant.[J]. Plant and Cell Physiology, 2003(3):255-261.
[25]KIM S R, YANG J I, AN G. OsCpn60α1, encoding the plastid chaperonin 60α subunit, is essential for folding of rbcL[J]. Moleculer Cells, 2013, 35(5) :402-409.
[26]WU Q, ZHANG C, CHEN Y, et al. OsCpn60β1 is essential for chloroplast development in rice (Oryza sativa L.)[J]. International Journal of Molecular Sciences, 2020, 21(11) :4023.
[27]KOUMOTO Y, TSUGEKI R, SHIMADA T, et al. Isolation and characterization of a cDNA encoding mitochondrial chaperonin 10 from Arabidopsis thaliana by functional complementation of an Escherichia coli groES mutant[J]. Plant J,1996, 10(6): 1119-1125.
[28]BEN-ZVI A P, GOLOUBINOFF P. Review: mechanisms of disaggregation and refolding of stable protein aggregates by molecular chaperones[J]. J Struct Biol, 2001, 135(2): 84-93.
[29]HU C, LIN S Y, CHARNG C, et al. Recent gene duplication and subfunctionalization produced a mitochondrial GrpE, the nucleotide exchange factor of the Hsp70 complex, specialized in thermotolerance to chronic heat stress in Arabidopsis[J]. Plant Physiology, 2012, 158(2):747-758.

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备注/Memo

备注/Memo:
收稿日期:2020-01-25基金项目:湖南省自然科学基金项目(2020JJ4030);湖南省创新平台与人才计划项目(2020NK4222)作者简介:张斌(1981-),男,湖南永州人,博士,讲师,主要从事植物发育生物学研究,(E-mail)zhangbin27104@163.com通讯作者:袁志辉,(E-mail)zhh_yuan@126.com
更新日期/Last Update: 2021-09-06