[1]殷杰,赵永祥,薛江东,等.核糖体蛋白L12(RPL12)在猪繁殖与呼吸综合征病毒复制中的作用[J].江苏农业学报,2021,(03):686-693.[doi:doi:10.3969/j.issn.1000-4440.2021.03.017]
　YIN Jie,ZHAO Yong-xiang,XUE Jiang-dong,et al.Role of ribosomal protein L12（RPL12） in porcine reproductive and respiratory syndrome virus replication[J].,2021,(03):686-693.[doi:doi:10.3969/j.issn.1000-4440.2021.03.017]
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核糖体蛋白L12(RPL12)在猪繁殖与呼吸综合征病毒复制中的作用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年03期
页码:
686-693
栏目:
畜牧兽医·水产养殖
出版日期:
2021-06-30

文章信息/Info

Title:
Role of ribosomal protein L12（RPL12） in porcine reproductive and respiratory syndrome virus replication
作者:
殷杰1; 2; 赵永祥2; 薛江东1; 刘锴1; 李彬2; 马德慧1
（1.内蒙古民族大学动物科技学院，内蒙古通辽028000；2.江苏省农业科学院兽医研究所，江苏南京210014）
Author(s):
YIN Jie1; 2; ZHAO Yong-xiang2; XUE Jiang-dong1; LIU Kai1; LI Bin2; MA De-hui1
(1.College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China;2.Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
猪繁殖与呼吸综合征病毒（PRRSV）; 核糖体蛋白L12(RPL12); PRRSV GP2b蛋白
Keywords:
porcine reproductive and respiratory syndrome virus(PRRSV); ribosomal protein L12 (RPL12); PRRSV GP2b protein
分类号:
S852.65
DOI:
doi:10.3969/j.issn.1000-4440.2021.03.017
文献标志码:
A
摘要:
为研究宿主细胞核糖体蛋白L12（RPL12）在猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus, PRRSV）复制中的作用，本研究通过荧光定量PCR和Western-blotting等方法，检测了PRRSV感染对Marc-145细胞中RPL12表达的影响，以及过表达或敲减RPL12对PRRSV复制的影响。结果显示，PRRSV感染可上调Marc-145细胞中RPL12基因的表达。过表达RPL12可促进PRRSV的复制，而敲减RPL12可抑制PRRSV的复制。通过免疫共沉淀和共聚焦显微镜检测，进一步研究发现RPL12蛋白与PRRSV GP2b蛋白存在相互作用，并共定位于细胞质中。本研究首次发现了宿主蛋白RPL12能够促进PRRSV复制，并与PRRSV GP2b蛋白存在相互作用，为进一步研究PRRSV的感染与复制机制以及与宿主的相互作用提供了新的切入点，为抗病毒药物的研发提供了有益的探索。
Abstract:
To study the role of host cell ribosomal protein L12 (RPL12) in the replication of porcine reproductive and respiratory syndrome virus (PRRSV), real-time quantitative PCR and Western-blotting were performed to detect the effect of PRRSV infection on the expression of RPL12 in Marc-145 cells and the effect of over-expression and knockdown of RPL12 on the replication of PRRSV. The results showed that PRRSV infection could up-regulate the expression of RPL12 in Marc-145 cells. Over-expression of RPL12 promoted the replication of PRRSV, while knockdown of the RPL12 inhibited the replication of PRRSV. Through co-immunoprecipitation and confocal microscopy analysis, further study found that RPL12 protein interacted with PRRSV GP2b protein and co-localized in the cytoplasm. This study reported for the first time that the host protein RPL12 could promote PRRSV replication and interact with PRRSV GP2b protein, which provides a new cut-in point for further research on the mechanism of PRRSV infection and replication, and also provides a beneficial exploration for the development of antiviral drugs.

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备注/Memo

备注/Memo:
收稿日期：2020-01-04基金项目：国家自然科学基金项目（31460662） ；内蒙古民族大学硕士研究生科研创新项目（NMDSS1942）作者简介：殷杰（1994-），女，内蒙古通辽人，硕士研究生，主要从事动物病毒学研究。赵永祥为共同第一作者。通讯作者：马德慧，(E-mail)mdh1962@163.com;李彬，(E-mail)libinana@126.com
更新日期/Last Update: 2021-07-05