[1]高阳,侯非凡,熊雄,等.基于候选基因开发gSSR标记加密黄花菜和萱草种间遗传图谱[J].江苏农业学报,2021,(01):139-149.[doi:doi:10.3969/j.issn.1000-4440.2021.01.018]
　GAO Yang,HOU Fei-fan,XIONG Xiong,et al.Enhancement of the interspecific genetic map density for Hemerocallis citrina and H. fulva by developing gSSR markers based on candidate genes[J].,2021,(01):139-149.[doi:doi:10.3969/j.issn.1000-4440.2021.01.018]
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基于候选基因开发gSSR标记加密黄花菜和萱草种间遗传图谱()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年01期
页码:
139-149
栏目:
园艺
出版日期:
2021-02-28

文章信息/Info

Title:
Enhancement of the interspecific genetic map density for Hemerocallis citrina and H. fulva by developing gSSR markers based on candidate genes
作者:
高阳1; 2; 侯非凡1; 2; 3; 熊雄1; 2; 公菲菲1; 2; 王金耀1; 2; 3; 邢国明1; 2; 3; 李森1; 2; 3
（1.山西农业大学园艺学院，山西太谷030801；2.大同黄花产业发展研究院，山西大同037004；3.山西省设施蔬菜产业提质增效协同创新中心，山西太谷030801）
Author(s):
GAO Yang1; 2; HOU Fei-fan1; 2; 3; XIONG Xiong1; 2; GONG Fei-fei1; 2; WANG Jin-yao1; 2; 3; XING Guo-ming1; 2; 3; LI Sen1; 2; 3
（1.College of Horticulture，Shanxi Agricultural University，Taigu 030801，China；2.Datong Huanghua Industry Development Research Institute，Datong 037004，China；3.Collaborative Innovation Center of Quality and Profit Improvement for the Facility Vegetables of Shanxi Province，Taigu 030801，China）
关键词:
黄花菜; gSSR; 标记开发; 图谱加密
Keywords:
Hemerocallis citrina; gSSR; marker development; density enhancement of the genetic linkage map
分类号:
Q755
DOI:
doi:10.3969/j.issn.1000-4440.2021.01.018
文献标志码:
A
摘要:
为了更加精准地开展黄花菜基因组组装和性状定位，在课题组前期构建的大同黄花×摇篮曲种间遗传图谱的基础上，基于大同黄花全基因组和转录组数据库，针对秋水仙碱及昼夜节律2个关键性状所涉及的候选Unigene开发基因组SSR标记，进一步通过55个F1后代加密图谱。结果表明，新开发的95个gSSR标记中有46个标记在亲本及子代间具有多态性，多态性比例为48.42%，有22个gSSR标记到图谱上，同时有12个前期未上图的EST-SSR标记加密到新图谱。加密后的遗传图谱包含255个标记，由9个连锁群组成，总图距为3 142.64 cM，平均图距为11.88 cM，各连锁群标记数量为5～58个。研究结果为进一步研究黄花菜秋水仙碱含量及昼夜节律的功能基因定位奠定了重要基础。
Abstract:
To carry out genome assembly and character mapping of Hemerocallis citrina more accurately, genomic simple sequence repeats (SSR) markers were developed for the candidate Unigenes involved in two key characters (colchicine and circadian rhythm), on the basis of the interspecific genetic map of DatongHuanghua×Lullaby Baby constructed by our research group previously and the whole genome and transcriptome database of DatongHuanghua. The map density was further enhanced through 55 F1 progenies. The results showed that 46 of the 95 newly developed gSSR markers were polymorphic between parents and offsprings, with a polymorphic ratio of 48.42%. 22 gSSR markers were added to the map, while 12 EST-SSR markers not mapped earlier were added to the new map. The updated genetic map contained 255 markers which was composed of nine linkage groups. The total map distance was 3 142.64 cM, and the average map distance was 11.88 cM. The number of markers in each linkage group ranged from 5 to 58. The results can make important foundation for further study on colchicine content and functional gene mapping of circadian rhythm in H.citrina.

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备注/Memo

备注/Memo:
收稿日期：2020-06-26基金项目：山西农业大学青年拔尖创新人才支持计划项目（BJRC201601）；山西省农业重点研发计划重点项目（201703D211001-04-05、201903D211011-01)；山西省高等学校科技创新项目（2019L0375）作者简介：高阳（1995-），男，山西朔州人，硕士研究生，主要从事园艺植物生物技术与遗传改良方面的研究。（E-mail）sau_gaoyang@163.com通讯作者：李森，（E-mail）saulisen@163.com
更新日期/Last Update: 2021-03-15