[1]徐臣善,徐爱红,萧蓓蕾,等.授粉品种对红富士苹果果实糖积累及其代谢相关酶活性的影响[J].江苏农业学报,2021,(01):121-128.[doi:doi:10.3969/j.issn.1000-4440.2021.01.016]
　XU Chen-shan,XU Ai-hong,XIAO Bei-lei,et al.Effects of pollination varieties on sugar accumulation and metabolism related enzyme activities in red Fuji apple fruit[J].,2021,(01):121-128.[doi:doi:10.3969/j.issn.1000-4440.2021.01.016]
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授粉品种对红富士苹果果实糖积累及其代谢相关酶活性的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年01期
页码:
121-128
栏目:
园艺
出版日期:
2021-02-28

文章信息/Info

Title:
Effects of pollination varieties on sugar accumulation and metabolism related enzyme activities in red Fuji apple fruit
作者:
徐臣善1; 徐爱红1; 萧蓓蕾1; 赵海洲2; 潘恩敬1; 王明友1
（1.德州学院，山东德州253023；2.德州市林业事业发展中心，山东德州253023）
Author(s):
XU Chen-shan1; XU Ai-hong1; XIAO Bei-lei1; ZHAO Hai-zhou2; PAN En-jing1; WANG Ming-you1
(1.Dezhou University, Dezhou 253023, China;2.Dezhou Forestry Development Center, Dezhou 253023, China)
关键词:
苹果; 授粉品种; 糖积累; 糖代谢; 酶活性
Keywords:
apple; pollination variety; sugar accumulation; sugar metabolism; enzymatic activity
分类号:
S661.1
DOI:
doi:10.3969/j.issn.1000-4440.2021.01.016
文献标志码:
A
摘要:
以长富2号红富士苹果（Malus pumila Mill. cv. Red Fuji, Nagafu NO.2）为母本，红星、荷红、美红3个授粉品种为父本，进行人工授粉，研究授粉品种对红富士苹果果实糖积累及代谢关键酶活性的影响。结果表明：果实生长发育过程中，果实总糖、蔗糖、葡萄糖、果糖含量均呈上升的趋势，果糖占总糖的比例最高；3个授粉处理的果实蔗糖、葡萄糖和果糖含量在花后14～42 d均无显著差异，总糖含量在花后14 d无显著差异；花后70～182 d，授粉品种为美红的果实蔗糖含量显著低于授粉品种为红星的果实（P<0.05），葡萄糖和果糖含量则显著高于授粉品种为红星的果实（P<0.05），总糖含量在花后42～182 d显著高于授粉品种为红星的果实（P<0.05）。授粉品种为美红的果实蔗糖磷酸合酶（SPS）活性在花后154～182 d显著低于授粉品种为红星的果实（P<0.05），其他时间各授粉处理间无显著差异；授粉品种为美红的果实山梨醇脱氢酶（SDH）活性在花后70～182 d显著高于授粉品种为红星的果实（P<0.05），蔗糖合酶合成方向（SS-s）活性在花后70～182 d则显著低于授粉品种为红星的果实（P<0.05），酸性转化酶（AI）活性在整个发育期（花后14～182 d）显著高于授粉品种为红星的果实（P<0.05）；各授粉处理果实中性转化酶活性（NI）在整个发育期均无显著差异。果实发育过程中，所有各授粉处理果实糖的积累均与SDH、SPS、SS-s活性呈正相关，与NI活性呈负相关，与AI活性呈显著（P<0.05）或极显著负相关（P<0.01）。果实发育过程中，授粉品种没有影响红富士苹果果实糖积累及代谢酶活性的变化趋势，但影响糖含量及代谢酶活性，酶活性的改变可能导致了果实糖积累的差异。
Abstract:
The effects of pollination varieties on sugar accumulation in Red Fuji apple fruits and activities of key enzymes in metabolism were studied by using Red Fuji apple Nagafu NO.2 as the female parent and three pollination varieties (Starking, Hehong and Meihong) as the male parent to conduct artificial pollination. The results showed that the contents of total sugar, sucrose, glucose and fructose in fruits all presented rising trends during the process of growth and development, and fructose content accounted for the highest proportion of total sugar. There were no significant differences in sucrose, glucose and fructose content in fruits by three pollination treatments 14-42 days after bloom, and there was no significant difference in total sugar content 14 days after bloom. The sucrose content in fruits pollinated with Meihong was significantly lower than fruits pollinated with Starking (P<0.05), but glucose and fructose contents in fruits were both significantly higher than fruits pollinated with Starking (P<0.05), 70-182 days after bloom. The total sugar content in fruits pollinated with Meihong was significantly higher than fruits pollinated with Starking 42-182 days after bloom (P<0.05). The activity of sucrose phosphate synthase (SPS) in fruits pollinated with Meihong was significantly lower than fruits pollinated with Starking 154-182 days after bloom (P<0.05), but there was no significant difference between pollination treatments in other periods. The activity of sorbitol dehydrogenase (SDH) in fruits pollinated with Meihong was significantly higher than fruits pollinated with Starking (P<0.05), but the activity of synthesis of sucrose synthase (SS-s) was significantly lower than fruits pollinated with Starking (P<0.05), 70-182 days after bloom. The activity of acid invertase (AI) in fruits pollinated with Meihong was significantly higher than fruits pollinated with Starking (P<0.05) during the whole development period (14-182 days after bloom). There was no significant difference in activity of neutral invertase (NI) in fruits treated with different varieties during the whole developmental period. During the development process of fruits, the sugar accumulations showed positive correlations with SDH, SPS and SS-s activities in fruits pollinated with each variety, but showed negative correlation with NI activity and significant (P<0.05) or highly significant (P<0.01) negative correlations with AI activity. During the development process of fruits, pollination variety showed no variation trend on influencing sugar accumulation and related enzyme activities in sugar metabolism in fruits of Red Fuji apple. However, the pollination varieties affected sugars contents and metabolic enzyme activities in sugar metabolism of fruits and the changes of enzyme activities probably resulted in the differences in sugar accumulation of fruits.

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备注/Memo

备注/Memo:
收稿日期：2020-05-16基金项目：山东省自然科学基金项目（ZR2014CL025）；国家星火计划项目（2015GA740088）作者简介：徐臣善（1983-），男，山东日照人，博士，讲师，主要从事果树栽培生理、生态研究。（E-mail）michael_10@163.com通讯作者：王明友，（E-mail）nwmy_sddz@163.com
更新日期/Last Update: 2021-03-15