[1]裴徐梨,荆赞革,徐境,等.青花菜BoDof5.3基因的克隆及渍水胁迫表达特征分析[J].江苏农业学报,2020,(06):1498-1502.[doi:doi:10.3969/j.issn.1000-4440.2020.06.020]
　PEI Xu-li,JING Zan-ge,XU Jing,et al.Cloning and expression analysis of BoDof5.3 gene under waterlogging stress in broccoli[J].,2020,(06):1498-1502.[doi:doi:10.3969/j.issn.1000-4440.2020.06.020]
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青花菜BoDof5.3基因的克隆及渍水胁迫表达特征分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年06期
页码:
1498-1502
栏目:
园艺
出版日期:
2020-12-31

文章信息/Info

Title:
Cloning and expression analysis of BoDof5.3 gene under waterlogging stress in broccoli
作者:
裴徐梨1; 荆赞革1; 2; 徐境1; 唐征2; 宋波3
(1.昆明学院农学与生命科学学院，云南昆明650214；2.温州科技职业学院农业与生物技术学院，浙江温州325006；3.江苏省农业科学院蔬菜研究所，江苏南京210014)
Author(s):
PEI Xu-li1; JING Zan-ge1; 2; XU Jing1; TANG Zheng2; SONG Bo3
(1.College of Agriculture and Life Science, Kunming University, Kunming 650214, China;2.College of Agriculture and Biotechnology, Wenzhou Vocational College of Science and Technology, Wenzhou 325006, China;3.Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
青花菜; Dof基因; 基因克隆; 渍水胁迫; 表达特征
Keywords:
Brassica oleracea var. italica; Dof gene; gene clone; waterlogging stress; expression feature
分类号:
S635.3
DOI:
doi:10.3969/j.issn.1000-4440.2020.06.020
文献标志码:
A
摘要:
从青花菜中克隆得到一个Dof基因，命名为BoDof5.3。序列分析结果表明：该基因ORF全长774 bp，编码257个氨基酸，编码的蛋白质为亲水性蛋白质。青花菜BoDof5.3蛋白具有典型的Zf-Dof结构域，其二级结构以无规则卷曲为主。系统进化分析结果表明，青花菜BoDof5.3蛋白与甘蓝、花椰菜Dof蛋白亲缘关系最近，在进化树上聚为一组, 同属植物的Dof蛋白具有较近的亲缘关系。qRT-PCR分析结果显示，在渍水胁迫0 d到2 d时，BoDof5.3基因表达量呈下降趋势，6 d时表达量升高为对照的1.3倍，推测该基因可能参与青花菜渍水胁迫的响应。
Abstract:
In this study, a Dof gene named BoDof5.3 was cloned from broccoli. Sequence analysis results showed that this gene was 774 bp in length, which encoded 257 amino acids. Furthermore, the protein encoded by this gene was a hydrophilic protein. The BoDof5.3 contained a typical Zf-Dof domain, and its main secondary structure was the random coil. Results of phylogenetic analysis indicated that the BoDof5.3 protein of broccoli had the highest homology with the Dof protein of cabbage and cauliflower, and they were clustered into a group on phylogenetic tree. Furthermore, the Dof protein in the same genus had close relationship. The results of qRT-PCR showed that the expression level of BoDof5.3 decreased from 0 d to 2 d under waterlogging stress, and the expression level at six days of waterlogging stress was 1.3 times as much as that of control. It is speculated that BoDof5.3 gene may be involved in the response of broccoli to waterlogging stress.

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备注/Memo

备注/Memo:
收稿日期：2020-03-21基金项目：浙江省自然科学基金项目(LY18C150006)；云南省地方本科高校(部分)基础研究联合专项资金项目(2018FH001-044)；云南省基础研究计划青年项目(2019-1-C-25318000002236)；浙江省科技厅项目(2016C051-5-3)；温州市科技项目(2019ZX007-2)作者简介：裴徐梨(1990-)，女，云南个旧人，博士，讲师，主要从事蔬菜分子生物学研究。(E-mail)xuliP1990@163.com通讯作者：荆赞革，(E-mail)jingzange@aliyun.com
更新日期/Last Update: 2021-01-15