[1]陈丹艳,周威,段婧婧,等.水生植物对不同形态氮素污水的生理响应及相应脱氮效率[J].江苏农业学报,2020,(06):1468-1474.[doi:doi:10.3969/j.issn.1000-4440.2020.06.016]
 CHEN Dan-yan,ZHOU Wei,DUAN Jing-jing,et al.The physiological response of aquatic plants to sewage with different forms of nitrogen and the corresponding nitrogen removal efficiency[J].,2020,(06):1468-1474.[doi:doi:10.3969/j.issn.1000-4440.2020.06.016]
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水生植物对不同形态氮素污水的生理响应及相应脱氮效率()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年06期
页码:
1468-1474
栏目:
耕作栽培·资源环境
出版日期:
2020-12-31

文章信息/Info

Title:
The physiological response of aquatic plants to sewage with different forms of nitrogen and the corresponding nitrogen removal efficiency
作者:
陈丹艳1周威12段婧婧2薛利红2冯彦房2
(1.金陵科技学院园艺园林学院,江苏南京210038;2.江苏省农业科学院农业资源与环境研究所,农业农村部长江下游平原农业环境重点实验室,江苏南京210014)
Author(s):
CHEN Dan-yan1ZHOU Wei12DUAN Jing-jing2XUE Li-hong2FENG Yan-fang2
(1.College of Horticulture, Jinling Institute of Technology, Nanjing 210038, China;2.Key laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
黄花水龙铜钱草氮素形态生理响应抗氧化酶
Keywords:
Jussiaea stipulacea OhwiHydrocotyle verticillatanitrogen formphysiological responseantioxidant enzyme
分类号:
X592
DOI:
doi:10.3969/j.issn.1000-4440.2020.06.016
文献标志码:
A
摘要:
为了探究黄花水龙(Jussiaea stipulacea Ohwi)和铜钱草(Hydrocotyle verticillata)在不同形态氮素污水中的抗逆性和适应性,开展了水箱模拟试验,模拟农田径流污水[总氮(TN)质量浓度为8 mg/L,总磷(TP)质量浓度为1 mg/L],研究这2种水生植物对污水中TN、NO-3-N、NH+4-N的净化能力及其抗性生理响应,设置2种形态氮素:以NO-3-N为主(NO-3-N∶NH+4-N=4∶1)和以NH+4-N为主(NH+4-N∶NO-3-N=4∶1)。结果表明:(1)在2种形态氮素下2种水生植物处理对氮素去除率明显高于无水生植物的对照,其中黄花水龙对氮素去除率较高,在高NO-3-N和高NH+4-N形态下对TN的去除率分别为48%~69%和69%~91%;(2)对不同形态氮素污水,2种水生植物处理对NO-3-N的去除效果均高于NH+4-N,其中黄花水龙在高NO-3-N和高NH+4-N形态下对NO-3-N的去除率分别高达58%~66%和79%~91%,而对NH+4-N的去除率仅为-65%~25%和21%~62%;(3)夏季试验期间,高质量浓度氮素增加了黄花水龙和铜钱草的氧化胁迫作用,2种水生植物通过调节体内抗氧化酶机制来响应。铜钱草体内的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性在不同形态氮素下除第1周外均高于黄花水龙,表明在外界高质量浓度氮条件下,铜钱草受到了较大的胁迫;高NO-3-N形态下黄花水龙的蛋白质和叶绿素含量在试验过程中均高于铜钱草,表明在高硝氮污水胁迫下黄花水龙耐受性更好。
Abstract:
In order to explore the stress resistance and adaptability of Jussiaea stipulacea Ohwi and Hydrocotyle verticillata in sewage with different forms of nitrogen, a water tank experiment was carried out. Using simulated farmland runoff wastewater(the mass concentration of total nitrogen was 8 mg/L, the mass concentration of total phosphorus was 1 mg/L),two forms of nitrogen were set:mainly NO-3-N(NO-3-N∶NH+4-N=4∶1) and NH+4-N(NH+4-N∶NO-3-N=4∶1),to study the purification ability of these two aquatic plants to TN, NO-3-N and NH+4-N in sewage and its resistance physiological response. The results showed that under the two forms of nitrogen, the nitrogen removal rates in the two aquatic plants treatments were significantly higher than those in the control, and the nitrogen removal rate of Jussiaea stipulacea Ohwi was the highest, and the removal rates of TN were 48%-69% and 69%-91% under high NO-3-N and high NH+4-N conditions, respectively. In wastewater with different forms of nitrogen, the removal efficiency of NO-3-N in two aquatic plants treatments was higher than that of NH+4-N. The NO-3-N removal rates of Jussiaea stipulacea Ohwi were 58%-66% and 79%-91% under the conditions of high NO-3-N and high NH+4-N, respectively, while the removal rates of NH+4-N were -65%-25% and 21%-62%, respectively. The high concentration of nitrogen increased the oxidative stress of Jussiaea stipulacea Ohwi and Hydrocotyle verticillata. The two aquatic plants responded to the stress by regulating the antioxidant enzyme mechanism. The activities of superoxide dismutase(SOD)and peroxidase(POD)in Hydrocotyle verticillata were higher than those in Jussiaea stipulacea Ohwi in each cycle except the first week, indicating that under high nitrogen conditions, Hydrocotyle verticillata was under greater stress. Under the conditions of high NO-3-N, the contents of protein and chlorophyll in Jussiaea stipulacea Ohwi were higher than those in Hydrocotyle verticillata during the experiment, indicating that the Jussiaea stipulacea Ohwi was more tolerant under high nitrate wastewater stress.

参考文献/References:

[1]杨林章,冯彦房,施卫明,等. 我国农业面源污染治理技术研究进展[J].中国生态农业学报,2013,21(1):96-101.
[2]欧阳威,蔡冠清,黄浩波,等. 小流域农业面源氮污染时空特征及与土壤呼吸硝化关系分析[J].环境科学,2014,35(6):2411-2418.
[3]宋立芳,王毅,吴金水,等. 水稻种植对中亚热带红壤丘陵区小流域氮磷养分输出的影响[J].环境学,2014,35(1):150-156.
[4]张芳,易能,张振华,等. 不同类型水生植物对富营养化水体氮转化及环境因素的影响[J].江苏农业学报,2015,31(5):1045-1052.
[5]陈友媛,崔香,董滨,等. 3种水培观赏植物净化模拟污水的试验研究[J].水土保持学报,2011,25(2):253-257.
[6]常会庆,王世华,徐晓峰,等. 两种水生植物对重富营养化水体修复效果研究[J].水土保持研究,2012,19(5):262-265.
[7]邵凯迪,段婧婧,薛利红,等. 5种水生植物对模拟菜地径流中总氮和硝氮净化效果[J].环境工程技术学报,2020,10(3):406-413.
[8]李敏,张健,李玉娟,等. 植物耐盐生理及耐盐基因的研究进展[J]. 江苏农业科学,2012,40(10):45-49.
[9]熊汉锋,谭启玲,刘艳玲. 不同营养状态下苦草的生理响应[J].华中农业大学学报,2009,28(4):442-445.
[10]孙瑞莲,刘健. 3种挺水植物对污水的净化效果及生理响应[J].生态环境学报,2018,27(5):926-932.
[11]王超,张文明,王沛芳,等. 黄花水龙对富营养化水体中氮磷去除效果的研究[J].环境科学,2007(5):975-981.
[12]宋玉芝,黄瑾,秦伯强. 附着生物对太湖常见的两种沉水植物快速光曲线的影响[J].湖泊科学,2010,22(6):935-940.
[13]张艳娜,宋玉芝,王敏,等. 附植藻类对沉水植物狐尾藻生长及生理的影[J].环境科学学报,2015,35(7): 2282-2288.
[14]刘丹丹,李正魁,叶忠香,等. 伊乐藻和氮循环菌技术对太湖氮素吸收和反硝化的影响[J].环境科学,2014,35(10):3764-3768.
[15]刘志迎,许海,詹旭,等. 蓝藻水华对太湖水柱反硝化作用的影响[J].环境科学,2019,40(3):1261-1268.
[16]袁东海,高士祥,任全进,等. 几种挺水植物净化生活污水总氮和总磷效果的研究[J].水土保持学报,2004,18(4):77-80,92.
[17]陆庆楠,贺宇欣,庄文化,等. 粉绿狐尾藻净水效果对氮磷浓度的响应机制[J].中国农村水利水电,2019(2):11-15.
[18]刘凤梅. 温度对凤眼莲浮岛净化效果影响的研究[J].环境保护科学,2013,39(3):9-11,54.
[19]FRIDOVIEH I. The biology of oxygen radical[J].Science,1975,201:875-880.
[20]李合生. 植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[21]BOWLER C. Superoxide dismutase and stress tolerance[J]. Plant Molecular Biology,1992,43:83-91.
[22]熊汉锋,谭启玲,李伟,等. 不同营养状态下3种沉水植物的生理响应[J].海洋湖沼通报,2009(4):62-66.
[23]张柳. 南美天胡荽对光温胁迫的适应性及园林应用研究[D].长沙:中南林业科技大学,2014.
[24]HARE P D,CRESS W A,VAN S J. Dissecting the roles of osmolyte accumulation during stress[J].Plant Cell Environment,1998,21:535-553.
[25]付晓云,梁茵,黄彦青. 污染水体中水生植物的生理效应研究[J].西北林学院学报,2013,28(1):63-66,72.
[26]沙翠芸,孟庆瑞,王静,等. 两种彩叶植物对铅胁迫的生理响应[J]. 西北林学院学报, 2011, 26(4):36-40.
[27]李雅洁,张其安,陆晓民. 不同外源物质对低温弱光次生盐渍化复合逆境下黄瓜幼苗生长、抗氧化系统及光合作用的影响[J]. 江苏农业学报,2018,34(2):404-410.
[28]袁孟玲,岳堃,王红,等.增强UV-B辐射对芒果成年树光合作用及其产量与常规品质的影响[J].南方农业学报,2018, 49(5):930-937.
[29]高祺,李明,朴琳,等. 拔节期弱光和渍水胁迫对春玉米光合作用、根系生长及产量的影响[J].江苏农业学报,2018,34(6):1276-1286.
[30]朱新广,张其德. NaCl对光合作用影响的研究进展[J].植物学通报,1999,16(4):332-338.
[31]刘凤歧,刘杰淋,朱瑞芬,等. 4种燕麦对NaCl胁迫的生理响应及耐盐性评价[J].草业科学,2015,24(1):183-189.

备注/Memo

备注/Memo:
收稿日期:2020-07-08基金项目:国家水体污染控制与治理科技重大专项(2017ZX07203-003、2017ZX07202004-003);江苏省农业科技自主创新基金项目[CX(18)1005];江苏省科技计划重点研发(现代农业)项目(BE2019378);金陵科技学院高层次人才科研启动项目(jit-b-201914)作者简介:陈丹艳(1986-),女,江西南昌人,博士,工程师,主要从事农业水土环境保护、农业水肥资源高效利用等方面的研究。(Tel)025-85393314;(E-mail)chendanyan@163.com通讯作者:段婧婧,(E-mail)duanjingjing_23@163.com
更新日期/Last Update: 2021-01-15