[1]涂健,阮苑,蔡伟真,等.RyhB-cysE基因对禽致病性大肠杆菌生物表型的影响[J].江苏农业学报,2020,(05):1247-1254.[doi:doi:10.3969/j.issn.1000-4440.2020.05.023]
　TU Jian,RUAN Yuan,CAI Wei-zhen,et al.Effects of RyhB-cysE gene on biological characteristics of avian pathogenic Escherichia coli[J].,2020,(05):1247-1254.[doi:doi:10.3969/j.issn.1000-4440.2020.05.023]
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RyhB-cysE基因对禽致病性大肠杆菌生物表型的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年05期
页码:
1247-1254
栏目:
畜牧兽医·水产养殖
出版日期:
2020-10-31

文章信息/Info

Title:
Effects of RyhB-cysE gene on biological characteristics of avian pathogenic Escherichia coli
作者:
涂健; 阮苑; 蔡伟真; 宋祥军; 邵 颖; 祁克宗
（安徽农业大学兽医病理生物学与疫病防控安徽省重点实验室，安徽合肥230036）
Author(s):
TU Jian; RUAN Yuan; CAI Wei-zhen; SONG Xiang-jun; SHAO Ying; QI Ke-zong
(Key Laboratory of Veterinary Pathology and Disease Control and Prevention of Anhui Province, Anhui Agricultural University, Hefei 230036, China)
关键词:
禽致病性大肠杆菌; RyhB基因; cysE基因; 生物被膜; 运动性
Keywords:
avian pathogenic Escherichia coli; RyhB gene; cysE gene; biofilm; motility
分类号:
S851.3
DOI:
doi:10.3969/j.issn.1000-4440.2020.05.023
文献标志码:
A
摘要:
在禽致病性大肠杆菌（APEC）AE17及AE17△RyhB的基础上利用Red同源重组技术构建基因缺失株AE17△cysE、AE17△RyhB△cysE, 对野生株和基因缺失株的生长、生物被膜形成和运动特性进行分析，并采用qRT-PCR技术比较野生株和缺失株中与运动性、生物被膜形成相关基因的转录水平。结果显示，各菌株生长曲线无显著差异；AE17△RyhB、AE17△cysE、AE17△RyhB△cysE生物被膜形成能力较AE17显著下降；AE17△cysE 、AE17△RyhB△cysE运动能力与AE17相比下降；AE17△cysE、AE17△RyhB△cysE与AE17相比flhB 、flgD、fliF和 cheY转录水平均降低，而AE17△RyhB△cysE与AE17△cysE相比基因转录水平均有所增强；AE17△RyhB、AE17△cysE、AE17△RyhB△cysE与AE17相比，flhD 、flgC、sidA转录水平均显著降低。表明cysE基因缺失降低了APEC生物被膜形成能力及运动能力，且RyhB基因缺失对cysE基因的运动能力调控作用有代偿作用，RyhB、cysE基因均通过调节与生物被膜形成相关的基因（flhD 、flgC、sidA）转录水平调控APEC生物被膜形成能力，本研究结果为研究RyhB-cysE基因对APEC的调控作用奠定了基础。
Abstract:
Based on avian pathogenic Escherichia coli (APEC) AE17 and AE17△RyhB, gene-deleted strains AE17△cysE and AE17△RyhB△cysE were constructed using Red homologous recombination technology. The growth, biofilm formation and motion characteristics of wild and gene-deleted strains were analyzeds and qRT-PCR technology was used to compare the transcription levels of genes related to motility and biofilm formation in wild and gene-deleted strains. The results showed that there was no significant difference in the growth curve of each strain. The biofilm formation ability of AE17△RyhB, AE17△cysE, AE17△RyhB△cysE was significantly lower than that of AE17. Compared with AE17, the transcription levels of flhB, flgD, fliF and cheY in AE17△cysE and AE17△RyhB△cysE were reduced, while AE17△RyhB△cysE had enhanced gene transcription levels compared with AE17△cysE. The AE17△RyhB, AE17△cysE, AE17△RyhB△cysE and AE17 flhD, flgC and sidA were significantly reduced. The results indicated that the deletion of cysE gene reduced the ability of APEC biofilm formation and exercise, and RyhB gene had a compensatory effect on the regulation of cysE gene’s exercise capacity. RyhB and cysE genes regulated the biofilm formation ability of APEC by regulating transcription level of genes(flhD, flgC and sidA) related to biofilm formation. These results of this study lay a foundation for studying the regulatory effect of RyhB-cysE gene on APEC.

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备注/Memo

备注/Memo:
收稿日期：2020-03-07基金项目：国家自然科学基金青年科学基金项目（31502038）； 国家自然科学基金面上项目（31972644）作者简介：涂健（1980-），男，汉族，安徽安庆人，博士，副教授，主要从事动物疫病病理及生物防治等研究。（E-mail）tujian1980@126.com通讯作者：祁克宗，（E-mail）qkz@ahau.edu.cn
更新日期/Last Update: 2020-11-16