[1]殷剑美,王立,蒋璐,等.芋[Colocasiaesculenta (L.) Schott]的倍性水平及基因组特征解析[J].江苏农业学报,2019,(06):1284-1291.[doi:doi:10.3969/j.issn.1000-4440.2019.06.003]
　YIN Jian-mei,WANG Li,JIANG Lu,et al.Analysis of ploidy level and genome characterization on taro [Colocasia esculenta (L.)Schott][J].,2019,(06):1284-1291.[doi:doi:10.3969/j.issn.1000-4440.2019.06.003]
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芋[Colocasiaesculenta (L.) Schott]的倍性水平及基因组特征解析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年06期
页码:
1284-1291
栏目:
遗传育种·生理生化
出版日期:
2019-12-31

文章信息/Info

Title:
Analysis of ploidy level and genome characterization on taro [Colocasia esculenta (L.)Schott]
作者:
殷剑美; 王立; 蒋璐; 韩晓勇; 郭文琦; 李春宏; 张培通
（江苏省农业科学院经济作物研究所江苏省农业生物学重点实验室，江苏南京210014）
Author(s):
YIN Jian-mei; WANG Li; JIANG Lu; HAN Xiao-yong; GUO Wen-qi; LI Chun-hong; ZHANG Pei-tong
（Institute of Industrial Crops， Jiangsu Academy of Agricultural Sciences， Provincial Key Laboratory of Agrobiology， Nanjing 210014, China）
关键词:
芋; 流式细胞术; 基因组测序; 基因功能注释; 倍型水平; 基因组特征
Keywords:
Colocasia esculenta; flow cytometry; genome sequencing; gene function annotation; ploidy level; genomic characterization
分类号:
S632.3
DOI:
doi:10.3969/j.issn.1000-4440.2019.06.003
文献标志码:
A
摘要:
采用流式细胞术，对2个芋[Colocasiaesculenta (L.) Schott]品种苏芋1号和香蕉芋的倍性水平和DNA含量进行了测定，2倍基因组 DNA含量分别为7.71 pg和5.25 pg，倍性分别为三倍体和二倍体。同时通过HiSeq 2500测序，分别获得240.53 Gb和87.69 Gb数据，芋基因组大小为2.08～2.38 Gb，GC含量为42.57%～42.75%，杂合度为0.52%～2.29%。根据苏芋1号的基因组数据，组装得到基因组序列为2.27 Gb，Contig N50为359 bp；共注释获得24 302个基因，99.43%的基因可注释到6个公共数据库；筛选出928 987个SSR位点。
Abstract:
In this study, the ploidy levels and DNA contents of two Colocasia esculenta varieties, Suyu No.1 and Xiangjiaoyu from China, were determined by flow cytometry (FCM). The 2C DNA contents of them were 7.71 pg and 5.25 pg, respectively, which indicating that their ploidy levels were triploid and diploid. In total, 240.53 Gb and 87.69 Gb sequences were obtained through HiSeq 2500 sequencing methods, respectively. The genome size of C. esculenta was in the range of 2.08-2.38 Gb. The GC contentsr anged from 42.57% to 42.75%, and the heterozygosity was in the range of 0.52%-2.29%. According to the genomic data of Suyu No.1, the assembled genome sequence was about 2.27 Gb, and contig N50 was 359 bp. A total of 24 302 genes were generated, and 24163 (about 99.43%)gene sequences were successfully annotated from six public databases. A total of 928 987 SSR loci were screened out.

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备注/Memo

备注/Memo:
收稿日期：2019-04-01 基金项目：国家自然科学基金项目（31501776）；江苏省农业生物学重点实验室项目(4911707Z201707) 作者简介：殷剑美（1977-），女，江苏丹阳人，博士，研究员，主要从事药食同源作物育种与分子生物学研究。（Tel）025-84390860；（E-mail）yinjm2006@jaas.ac.cn 通讯作者：张培通，（E-mail）ptzhang1965@163.com
更新日期/Last Update: 2020-01-09