[1]宋祥军,程悦,邱明宇,等.禽致病性大肠杆菌phoP/Q基因缺失对雏鸡脾脏microRNAs功能的影响[J].江苏农业学报,2019,(05):1167-1174.[doi:doi:10.3969/j.issn.1000-4440.2019.05.024]
　SONG Xiang-jun,CHENG Yue,QIU Ming-yu,et al.Effect of avian pathogenic Escherichia coli phoP/Q gene mutation on microRNAs function in chicken spleen[J].,2019,(05):1167-1174.[doi:doi:10.3969/j.issn.1000-4440.2019.05.024]
点击复制

禽致病性大肠杆菌phoP/Q基因缺失对雏鸡脾脏microRNAs功能的影响()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年05期
页码:
1167-1174
栏目:
畜牧兽医·水产养殖
出版日期:
2019-10-31

文章信息/Info

Title:
Effect of avian pathogenic Escherichia coli phoP/Q gene mutation on microRNAs function in chicken spleen
作者:
宋祥军; 程悦; 邱明宇; 薛媚; 涂健; 邵颖; 祁克宗
(安徽农业大学兽医病理生物学与疫病防控安徽省重点实验室，安徽合肥230036)
Author(s):
SONG Xiang-jun; CHENG Yue; QIU Ming-yu; XUE Mei; TU Jian; SHAO Ying; QI Ke-zong
(Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agriculture University, Hefei 230036, China)
关键词:
禽致病性大肠杆菌; phoP/Q; 脾脏; miRNA; 差异表达
Keywords:
avian pathogenic Escherichia coli; phoP/Q; spleen; microRNA; differential expression
分类号:
S858.31
DOI:
doi:10.3969/j.issn.1000-4440.2019.05.024
文献标志码:
A
摘要:
从microRNA(miRNA)水平探究禽致病性大肠杆菌(APEC)phoP/Q基因缺失对雏鸡脾脏功能表达的影响，为APEC的防制提供参考资料。用禽致病性大肠杆菌和其phoP/Q基因的缺失株分别攻毒14日龄雏鸡，采集其脾脏组织为材料进行高通量测序获得miRNA表达谱，选择差异倍数大于8的miRNA进行Real-time PCR，对差异表达的miRNA进行靶基因预测以及GO、KEGG富集分析。测序结果获得10个差异表达的miRNA，其中1个miRNA表达量下调，9个miRNA表达量上调，Real-time PCR结果表明，miRNA变化趋势与测序结果一致，GO分析结果表明，差异表达基因主要富集在能量代谢、免疫系统、细胞生长与凋亡、糖合成与代谢等生物学过程。KEGG分析结果显示预测靶基因参与MAPK信号通路、糖代谢通路、Wnt信号通路等重要通路。本试验分析禽致病性大肠杆菌phoP/Q基因缺失后感染雏鸡脾脏miRNA的表达差异，从miRNA水平探讨了phoP/Q基因缺失对APEC致病性的影响，为APEC的防治提供参考资料。
Abstract:
The effect of avian pathogenic Escherichia coli (APEC) phoP/Q gene deletion on the expression of spleen function in chicks was investigated from the level of microRNA (miRNA), which provided reference for the prevention and control of APEC. The 14-day-old chicks were challenged with avian pathogenic Escherichia coli and their phoP/Q gene deletion strains, and their spleen tissues were collected for high-throughput sequencing to obtain miRNA expression profiles. The miRNAs with a fold difference greater than eight fold were selected for real-time PCR, target gene prediction and GO and KEGG enrichment analysis were carried out for differentially expressed miRNAs. Ten differentially expressed miRNAs were obtained by sequencing. One miRNA expression was down-regulated and nine miRNA expression levels were up-regulated. Real-time PCR results showed that the change trend of miRNA was consistent with sequencing results. GO analysis results showed that differentially expressed genes were mainly enriched in biological processes such as energy metabolism, immune system, cell growth and apoptosis, sugar synthesis and metabolism. KEGG analysis results showed that the predicted target genes were involved in important pathways, such as MAPK signaling pathway, glucose metabolism pathway and Wnt signaling pathway. In this study, the difference of microRNA expression in spleen of chickens infected with avian pathogenic Escherichia coli phoP/Q gene deletion was analyzed, and the influence of phoP/Q gene deletion on APEC pathogenicity was discussed from the level of microRNA in order to provide reference for the prevention and control of APEC.

参考文献/References:

[1]马兴树，朱美霞，石虎，等.禽致病性大肠杆菌的分离、O血清群鉴定及药物敏感试验[J].中国畜牧兽医，2016,43(12):3343-3348.
[2]王传春，袁东方，孙秋艳，等.禽致病性大肠杆菌的分离鉴定及致病性研究[J].山东畜牧兽医，2017,38(3):8-9.
[3]夏乐.禽致病性大肠杆菌E058株在鸡感染模型中的转录组学分析及sodA基因功能和致病性评价[D].扬州：扬州大学,2017.
[4]李春晓，张宇曦，祁克宗，等. phoP/Q双组分系统对禽致病性大肠杆菌的毒力调控作用[J].畜牧兽医学报，2016,47(1):157-164.
[5]黄博言.禽致病性大肠杆菌phoP/Q基因缺失对其致病性的影响[D].合肥：安徽农业大学,2015.
[6]薛媚，祁克宗，薛挺，等.利用基因芯片筛选禽致病性大肠杆菌中与phoP/Q二元调控系统相关的耐药基因[J].中国预防兽医学报，2017,39(8):672-674.
[7]LAVRIC M,MAUGHAN M N,BLISS T W,et al.Gene expression modulation in chicken macrophages exposed to Mycoplasma synoviae or Escherichia coli [J].Veterinary Microbiology,2007,126(1):111-121.
[8]JIA X Z,NIE Q H,ZHANG X Q,et al.Novel MicroRNA involved in host response to avian pathogenic Escherichia coli identified by deep sequencing and integration analysis[J].Infection and Immunity,2017,85(1):e00688.
[9]BARTEL D P.microRNAs:genomics，biogenesis，mechanism，and function[J].Cell，2004，116(2):281-297.
[10]BARTEL D P.microRNAs:genomics:target recognition and regulatory function[J].Cell,2009,136(2):215-233.
[11]KLOOSTERMAN W P,PLASTERK R H A.The diverse function of microRNAs in animal development and disease[J].Dev Cell,2006,11(4):441-450.
[12]TIRON A,VASILESCU C.Spleen and immunity immune implications of splenectomy[J].Chirurgia,2008,103(3):255-263.
[13]李基棕，李文良，毛立，等.山羊副流感病毒3型感染MDBK细胞的miRNA表达谱变化分析[J].畜牧兽医学报，2017,48(5):896-906.
[14]RODRIGUEZ A,VIGORITO E,CLARE S,et al.Requirement of bic/microRNA-155 for normal immune function[J].Science,2007,316(5824):608-611.
[15]黄超，吴海斌，杨娟，等.大肠杆菌病患鸡肝、脾结构的病理学研究[J].畜牧与饲料科学，2016,37(6/7):134-136.
[16]王福传，韩一超，赵洪恩，等.复方中草药免疫增强剂对鸡免疫器官组织形态学影响的研究[J].中国预防兽医学报，2001,23(6):419-421．
[17]ZHU S, PAN W, QIAN Y C. MicroRNA in immunity and autoimmunity[J]. Journal of Molecular Medicine, 2013,91(9):1039-1050.
[18]SANGFORD E E,ORR M,BALFANZ,et al.Spleen transcriptome response to infection with avian pathogenic Escherichia coli in broiler chickens[J].Bmc Genomics,2011,12(1):469.
[19]王惠珂，李少参，祁克宗，等.基于RNA-Seq筛选禽致病性大肠杆菌损伤雏鸡小肠差异表达基因[J].中国预防兽医学报，2016,38(2):111-115.
[20]王军，燕晓晓，丁赫，等.LPS诱导牛子宫内膜细胞miRNA差异表达分析[J].中国兽医杂志，2017,53(10):10-12,16.
[21]韩亚儒.马传染性贫血病毒感染靶细胞后差异表达microRNA的研究[D]. 呼和浩特：内蒙古农业大学,2018.
[22]陈严玉.新城疫病毒感染鸡巨噬细胞miRNA表达谱分析及gga-miRNA155的功能[D].长春：吉林大学,2018.
[23]ALMOG T,NAOR Z.The role of mitogen activated protein kinase (MAPK) in sperm functions[J].Mol Cell Endocrinol,2010,314(2):239-243.
[24]郭永娟，郑亚琳，张彩霞，等.MAPK信号通路在动物繁殖中的研究进展[J].中国畜牧兽医，2015,42(5):1268-1273.
[25]张秀秀，郭云涛，黄万龙，等.靶向MAPK信号通路调控脂肪细胞分化的microRNAs[J].畜牧兽医学报，2016,47(11):2159-2166.
[26]孙宏亮，许美花，王瑶，等.Wnt信号通路在牛胎盘发育过程中的功能研究进展[J].中国畜牧兽医，2017,44(1):161-166.
[27]LARA M K,LIN X.C-type lectin receptor-induced NF-κB activation in innate immune and inflammatory responses[J].Chinese Journal of Immunology,2012,9(2):105-112.
[28]杨婷，唐丽，贺福初.C型凝集素受体CLRs与固有免疫[J].细胞与分子免疫学杂志，2009,25(5):476-478.

相似文献/References:

[1]涂健,阮苑,蔡伟真,等.RyhB-cysE基因对禽致病性大肠杆菌生物表型的影响[J].江苏农业学报,2020,(05):1247.[doi:doi:10.3969/j.issn.1000-4440.2020.05.023]
　TU Jian,RUAN Yuan,CAI Wei-zhen,et al.Effects of RyhB-cysE gene on biological characteristics of avian pathogenic Escherichia coli[J].,2020,(05):1247.[doi:doi:10.3969/j.issn.1000-4440.2020.05.023]
[2]何琪,孙晨晨,侯曼曼,等.禽致病性大肠杆菌hcp2a基因对雏鸡脾脏细胞因子-细胞因子受体相互作用通路的影响[J].江苏农业学报,2022,38(01):151.[doi:doi:10.3969/j.issn.1000-4440.2022.01.018]
　HE Qi,SUN Chen-chen,HOU Man-man,et al.Effects of avian pathogenic Escherichia coli (APEC) hcp2a gene on the cytokine-cytokine receptor interaction pathway in chick spleen[J].,2022,38(05):151.[doi:doi:10.3969/j.issn.1000-4440.2022.01.018]
[3]吴剑梅,傅丹丹,李佳璇,等.ETT2转录因子EivF调控禽致病性大肠杆菌运动性和环境胁迫耐受性[J].江苏农业学报,2022,38(02):438.[doi:doi:10.3969/j.issn.1000-4440.2022.02.018]
　WU Jian-mei,FU Dan-dan,LI Jia-xuan,et al.Regulation of ETT2 transcription factor EivF on avian pathogenic Escherichia coli motility and tolerance to environmental stress[J].,2022,38(05):438.[doi:doi:10.3969/j.issn.1000-4440.2022.02.018]

备注/Memo

备注/Memo:
收稿日期：2018-12-28 基金项目：国家自然科学基金项目(31802161、31772707)； 安徽农业大学引进与稳定人才项目(yj2017-31)；安徽农业大学青年科学基金重点项目(2017zd01) 作者简介：宋祥军(1987-)，男，山东省临沭人，博士，讲师，主要从事动物疫病防控与生物防治研究。(E-mail)sxj@ahau.edu.cn 通讯作者：祁克宗，(E-mail)qkz@ahau.edu.cn
更新日期/Last Update: 2019-11-11