[1]赵娜,苗艳梅,赵敏.未知植物病毒分子生物学检测方法的研究现状[J].江苏农业学报,2019,(01):224-228.[doi:doi:10.3969/j.issn.1000-4440.2019.01.032]
　ZHAO Na,MIAO Yan-mei,ZHAO Min.Research status of molecular biological detection methods for unknown plant viruses[J].,2019,(01):224-228.[doi:doi:10.3969/j.issn.1000-4440.2019.01.032]
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未知植物病毒分子生物学检测方法的研究现状()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年01期
页码:
224-228
栏目:
综述
出版日期:
2019-02-26

文章信息/Info

Title:
Research status of molecular biological detection methods for unknown plant viruses
作者:
赵娜1; 2; 苗艳梅1; 赵敏1
(1.东北林业大学，黑龙江哈尔滨150040；2.白城师范学院，吉林白城137000)
Author(s):
ZHAO Na1; 2; MIAO Yan-mei1; ZHAO Min1
(1. Northeast Forestry University, Harbin 150040, China;2.Baicheng Normal University, Baicheng 137000, China)
关键词:
未知植物病毒; 分子生物学; 检测方法
Keywords:
unknown plant virus; molecular biology; detection methods
分类号:
S432.4+1
DOI:
doi:10.3969/j.issn.1000-4440.2019.01.032
文献标志码:
A
摘要:
植物的真菌病原菌可以通过核糖体RNA或者其他通用引物进行鉴定，植物病毒缺乏通用引物，这给植物未知病毒病的鉴定带来较大挑战。随着分子生物学及测序技术的发展，出现了很多直接从植物病料中检测未知病毒的方法，这些方法推进了植物病毒的发掘和病原鉴定，取得了非常好的效果。本文主要阐述近年来应用于检测未知植物病毒的分子生物学方法。
Abstract:
The fungal pathogen in plants can be identified by ribosomal RNA or other universal primers. Due to the lack of universal primers for plant viruses, the diagnosis of unknown viral diseases in plants encountered a great challenge. With the development of molecular biology and sequencing technology, there were many methods for detecting unknown viruses directly from plant diseases. The discovery and identification of plant viruses were promoted by using these methods, and good results were achieved. This paper reviews the application of molecular biological detection methods for unknown plant viruses in recent years.

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备注/Memo

备注/Memo:
收稿日期：2018-06-04 基金项目：国家自然科学基金项目（51678120） 作者简介：赵娜(1985-)，女，河南新乡人，博士，讲师，研究方向为微生物学。（E-mail）Bszhaona2013@163.com 通讯作者：赵敏，（E-mail）82191513@163.com
更新日期/Last Update: 2019-02-27