[1]李齐东,高璐,蒋建雄,等.嗜热内切葡聚糖酶（E1）植物亚细胞定位表达载体的构建及验证[J].江苏农业学报,2018,(06):1247-1253.[doi:doi:10.3969/j.issn.1000-4440.2018.06.007]
　LI Qi-dong,GAO Lu,JIANG Jian-xiong,et al.Construction and verification of the thermophilic endoglucanase (E1) subcellular plant expression vector[J].,2018,(06):1247-1253.[doi:doi:10.3969/j.issn.1000-4440.2018.06.007]
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嗜热内切葡聚糖酶（E1）植物亚细胞定位表达载体的构建及验证()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年06期
页码:
1247-1253
栏目:
遗传育种·生理生化
出版日期:
2018-12-25

文章信息/Info

Title:
Construction and verification of the thermophilic endoglucanase (E1) subcellular plant expression vector
作者:
李齐东; 高璐; 蒋建雄; 武艳芳; 李霞; 王永丽; 刘瑶瑶; 孙建中
（江苏大学环境与安全工程学院，生物质能源研究所，江苏镇江212013）
Author(s):
LI Qi-dong; GAO Lu; JIANG Jian-xiong; WU Yan-fang; LI Xia; WANG Yong-li; LIU Yao-yao; SUN Jian-zhong
（Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China）
关键词:
嗜热内切葡聚糖酶; 亚细胞定位; 瞬时表达
Keywords:
thermophilic endoglucanase; subcellular localization; transient expression
分类号:
Q78
DOI:
doi:10.3969/j.issn.1000-4440.2018.06.007
文献标志码:
A
摘要:
嗜热纤维素降解酶在植物中的表达对促进纤维素降解，提高生物质资源化利用具有重要作用。本研究采用来源于解纤维素热酸菌(Acidothermus cellulolytics）的嗜热内切葡聚糖酶基因（E1），基因序列优化后，构建了5种带有不同亚细胞定位信号肽的目的基因植物表达载体，分别将嗜热内切葡聚糖酶（E1）定位到细胞质、质外体、内质网、叶绿体和线粒体等亚细胞结构中。通过农杆菌介导的瞬时表达技术，利用洋葱表皮和烟草叶片进行定位表达研究，观察到在信号肽的引导下,基因表达产物在相应的亚细胞结构中正确定位。本研究为进一步探索E1不同定位表达在秸秆高效转化和资源化利用中的作用奠定了基础。
Abstract:
The expression of thermophilic cellulolytic enzymes in plants plays an important role in promoting the degradation of cellulose and improving the utilization of biomass. In this study, the thermophilic endoglucanase gene (E1) derived from Acidothermus cellulolytics was optimized. After optimization of the gene sequence, the thermophilic endoglucanase was localized to the cytoplasm, apoplast, endoplasmic reticulum, chloroplast and mitochondria by five binary plant expression vectors with different subcellular localization signal peptide, respectively. Agrobacterium-mediated transient expression technology was used to locate the target gene by using onion epidermis and tobacco. The subcellular localization of the gene expression products was observed under an inverted fluorescence microscope. This study laid the foundation for further exploration of the effects of different localization and expression of thermophilic endoglucanase on efficient transformation and resource utilization of straw.

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备注/Memo

备注/Memo:
收稿日期：2018-02-06 基金项目：国家自然科学基金项目（31601380、31671754）；江苏省高校优势学科建设工程项目；江苏大学高级人才基金项目（13JDB017） 作者简介：李齐东（1992-），男，山东聊城人，硕士研究生，主要从事秸秆和能源植物的资源化利用研究。(E-mail) rwwyrywy@126.com 通讯作者：孙建中，(E-mail)jzsun1002@ujs.edu.cn
更新日期/Last Update: 2018-12-28