[1]腾海艳.水稻Ossp1基因的亚细胞定位及其干旱条件下的表达[J].江苏农业学报,2020,(03):529-534.[doi:doi:10.3969/j.issn.1000-4440.2020.03.001]
　TENG Hai-yan.Subcellular localization and expression under drought conditions of rice Ossp1 gene[J].,2020,(03):529-534.[doi:doi:10.3969/j.issn.1000-4440.2020.03.001]
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水稻Ossp1基因的亚细胞定位及其干旱条件下的表达()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年03期
页码:
529-534
栏目:
遗传育种·生理生化
出版日期:
2020-06-30

文章信息/Info

Title:
Subcellular localization and expression under drought conditions of rice Ossp1 gene
作者:
腾海艳
（宜春学院化学与生物工程学院，江西宜春336000）
Author(s):
TENG Hai-yan
（School of Chemistry and Bioengineering， Yichun University， Yichun 336000， China）
关键词:
水稻; Ossp1基因; 亚细胞定位; 干旱胁迫; 基因表达
Keywords:
rice; Ossp1; subcellular localization;  drought stress; gene expression
分类号:
Q786
DOI:
doi:10.3969/j.issn.1000-4440.2020.03.001
文献标志码:
A
摘要:
sp1基因是叶绿体蛋白质输入调控的关键基因，本研究通过生物信息学分析、Real time PCR分析、瞬时表达分析等方法，对sp1在水稻中的同源基因Ossp1进行了结构和功能预测、组织表达和干旱响应性分析以及亚细胞定位分析。生物信息学分析结果显示，水稻Ossp1基因位于7号染色体，基因登录号为Os07g0647800，序列全长1 032 bp，SP1蛋白由343个氨基酸残基组成，信号肽序列位于氮端，蛋白质分子中有2个跨膜区域。Real time PCR分析结果显示，Ossp1在水稻叶片中表达量最高，其次为叶鞘，根中最低，此外，Ossp1在叶片中的表达具有明显的干旱响应性，受到干旱胁迫诱导后，表达量显著提高。采用瞬时表达进行亚细胞定位结果显示，SP1蛋白定位于水稻叶绿体。上述结果显示了Ossp1基因与水稻叶绿体及干旱胁迫响应的关系，为Ossp1基因功能的深入研究提供了基础。
Abstract:
The sp1 is a key gene for the regulation of chloroplast protein import, and Ossp1 is the homologous gene of sp1 in rice. In this study, the structure and function prediction, tissue expression, drought-responsiveness analysis and subcellular localization analysis of Ossp1 were carried out by bioinformatics analysis, realtime PCR analysis and transient expression analysis. The results of bioinformatics analysis showed that Ossp1 was located on chromosome 7(gene accession number: Os07g0647800), and the sequence was 1 032 bp in length. The SP1 protein was composed of 343 amino acid residues, the signal peptide was located at the N-terminus, and there were two transmembrane regions. The results of real time PCR analysis showed that the expression level of Ossp1 was highest in rice leaves, followed by leaf sheaths, and lowest in roots. In addition, the expression of Ossp1 could be significantly induced by drought stress, showed obvious drought responsiveness. Subcellular localization analysis of SP1 protein was performed by transient expression assay, and the results indicated that the SP1 protein was localized in rice chloroplasts. The above results show the relationship between gene Ossp 1 and rice chloroplast and drought responsiveness, and provide the basis for further research on the function of gene Ossp 1.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2019-11-21基金项目：江西省教育厅科学技术研究项目（GJJ180838）作者简介：腾海艳(1980-)，女，吉林白城人，博士，讲师，从事水稻逆境分子生理生化研究。（E-mail）tenghy477@163.com
更新日期/Last Update: 2020-07-14