[1]牛鹏飞,申远,李帅,等.苹果中福美胂残留的RP-HPLC检测[J].江苏农业学报,2018,(03):706-710.[doi:doi:10.3969/j.issn.1000-4440.2018.03.033]
　NIU Peng-fei,SHEN Yuan,LI Shuai,et al.Determination of residual asomate in apple by reversed-phase high-performance liquid chromatography (RP-HPLC)[J].,2018,(03):706-710.[doi:doi:10.3969/j.issn.1000-4440.2018.03.033]
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苹果中福美胂残留的RP-HPLC检测()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年03期
页码:
706-710
栏目:
加工贮藏·质量安全
出版日期:
2018-06-25

文章信息/Info

Title:
Determination of residual asomate in apple by reversed-phase high-performance liquid chromatography (RP-HPLC)
作者:
牛鹏飞; 申远; 李帅; 郭玉蓉
（陕西师范大学食品工程与营养科学学院， 陕西西安710062）
Author(s):
NIU Peng-fei; SHEN Yuan; LI Shuai; GUO Yu-rong
（College of Food Engineering and Nutritional Science, Shanxi Normal University, Xi’an 710062,China）
关键词:
RP-HPLC检测; 福美胂; 苹果
Keywords:
reversed-phase high-performance liquid chromatography (RP-HPLC) determination; asomate; apple
分类号:
X836
DOI:
doi:10.3969/j.issn.1000-4440.2018.03.033
文献标志码:
A
摘要:
为定量检测苹果中福美胂残留，建立一种基于紫外检测器的反相高效液相色谱检测方法。以苯为溶剂，震荡萃取苹果中福美胂，采用C18 色谱柱（250.0 mm×4.6 mm ID, 5.0 μm），以0.10%三氟乙酸水溶液为溶剂配制乙腈含量为10%溶液作为A相，100.00%乙腈为B相（A相∶B相=60∶40，体积比），在流速1.00 ml/min，柱温30 ℃，波长247 nm条件下进行福美胂色谱分析。结果表明，福美胂的加标回收率为 76.56%～100.22%，变异系数(CV)为 4.86%～7.51%，检出限（LOD）为0.295 3 mg/kg，定量限（LOQ）为1.206 7 mg/kg。该方法可用于苹果中福美胂残留检测。
Abstract:
A reversed-phase high-performance liquid chromatography (RP-HPLC) separation method with ultraviolet detector was developed to determine the residual asomate in apple. Using benzene as solvent, the asomate in apple was extracted. The asomate was determined by RP-HPLC using C18 (250.0 mm×4.6 mm ID, 5.0 μm) column and mobile phase consisting of A and B in the ratio of 60∶40 (V/V) with a flow rate of 1.00 ml/min at 30 ℃ in 247 nm. A was 0.10% trifluoroacetic acid aqueous solution-acetonitrile, B was 100.00% acetonitrile. The results showed that spiked recovery rate of asomate ranged from 76.56% to 100.22%, and coefficient of variation (CV) ranged from 4.86% to 7.51%. The limit of detection (LOD) was 0.295 3 mg/kg, and the limit of quantification (LOQ) was 1.206 7 mg/kg in apple. This method can be successfully used to measure residual asomate in apple.

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备注/Memo

备注/Memo:
收稿日期：2017-11-28 基金项目：农业部苹果产业技术体系项目(NYCYTX-08) 作者简介：牛鹏飞（1982-），男，陕西户县人，博士研究生，实验师，研究方向为食品安全及检测技术。（Tel）029-85310598；（E-mail）niupf0406@163.com 通讯作者：郭玉蓉，（Tel）029-85310471;（E-mail）guoyurong730@163.com
更新日期/Last Update: 2018-07-04